Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

Technical information on 2-D PAGE

Protocols

The Electrophoresis Laboratory at Geneva University Hospital has developed or improved various parts of the 2-D PAGE making process as well as post-separation analysis techniques. You may read the following protocols:

• Analytical 2-D PAGE protocols
  • Sample preparation
  • Immobilized pH gradient (IPG) as first dimension
  • SDS-PAGE as second dimension
  • Protein detection
• Preparative 2-D PAGE protocols
  • Sample preparation
  • Immobilized pH gradient (IPG) as first dimension
  • SDS-PAGE as second dimension
  • Protein electroblotting
  • Protein detection onto PVDF membranes
• Post-separation analysis
  • Sequencing
  • Amino acid composition
  • Sequence Tag
  • Peptide mass fingerprinting and amino acid composition identification
  • Immunoblotting
• Chemicals
• Apparatus
• References

Software

Learn about the MELANIE 2D PAGE computer analysis packages.

Courses

If you want to learn about the 2D PAGE technique, you may visit our laboratory and be taught by our experts. Please contact Jean-Charles Sanchez.