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Apparatus
- Equipment for isoelectric focusing (Reswelling cassette, Multiphor II electrophoresis chamber, Immobiline strip tray, Multidrive XL programmable power supply, Macrodrive 5 power supply and Multitemp II) was from Pharmacia-Hoeffer (Uppsala, Sweden).
- Equipments for vertical second dimension gels: Protean II casting chamber and Protean II chamber were from Bio-Rad (Richmond, CA), the Investigator system was from Millipore (Bedford, USA).
- The Angelique gradient pourer was from LargeScaleBiology (Rockville, MD).
- Electrotransfer equipment (Trans-Blott Cell) was from Bio-Rad (Richmond, CA).
- Equipment for microsequencing (ABI model 473A and 477A microsequencers) was from Applied Biosystems (Foster City, CA).
- Equipment for amino acid analysis (Aminomate Automated Analyser) was from GBC (Sydney, Australia).
- Equipment for mass spectrometry (LC-ESI-MS and MALDI-TOF-MS) was from PerSeptive Biosystems (Framingham, MA).
- The Laser Densitometer is from Molecular Dynamics and the GS-700 from Bio-Rad (Hercules, CA)
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