Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.
SWISS-2DPAGE Release 19.0
23 May 2011
Release 19.0 of the SWISS-2DPAGE database of proteins identified on 2-D and 1-D PAGE is now available.
The current release contains 1265 entries in 36 reference maps from human (Liver, Plasma, HepG2, Red blood cells, Lymphoma, HepG2 Secreted Proteins, Cerebrospinal Fluid, Macrophage Like Cell Line, Erythroleukemia Cell, Platelet, Kidney, Promyelocytic Leukemia Cells, Colorectal epithelia cells, Colorectal adenocarcinoma cell line (DL-1), Soluble nuclear proteins and matrix from liver tissue,
SDS-PAGE and 2-D PAGE of nucleolar proteins from Human HeLa cells, Lymphocyte,
from mouse (Liver, Gastrocnemius muscle, Pancreatic islet cells, Brow adipose tissue, White adipose tissue, Soluble nuclear proteins and matrix from liver tissue),
Arabidopsis thaliana, Dictyostelium discoideum,
Escherichia coli (for 7 pI ranges: 3.5 - 10, 4 - 5, 4.5 - 5.5, 5 - 6, 5.5 - 6.7, 6 - 9, 6 - 11; and DIGE 4.5 - 6.5),
Saccharomyces cerevisiae and
Staphylococcus aureus.
1. Availability of SWISS-2DPAGE
Through the ExPASy server: the most efficient and user-friendly way to browse interactively in SWISS-2DPAGE, with active links to other databases.
SWISS-2DPAGE has been updated using the new version of the Make2D-DB II package.
Make2D-DB II is a freely available tool to create, convert, interconnect and keep up-to-date proteomics databases; which has been developed by the Swiss Institute of Bioinformatics.
We are continuing on our efforts in promoting the large proteomics data network, with
high-level annotations, and where SWISS-2DPAGE and remote proteomics
databases are searchable from a single point of access. The
World-2DPAGE Portal is available as a portal to the
accessible proteomics databases, also built with the Make2D-DB package.
World-2DPAGE Portal is accessible at: http://world-2dpage.expasy.org/portal where one can query simultaneously a large set of proteomics databases, in addition to SWISS-2DPAGE.
The World-2DPAGE Repository (http://world-2dpage.expasy.org/repository.) continues to serve the proteomics community in hosting more and more data for publication and review.
should you have data you may want to share with fellow scientists.
5. Current content of the SWISS-2DPAGE database
Master
Creation
Last modified
Detected spots
Identified spots
No. of Entries
ARABIDOPSIS
Dec-00
May-03
1111
79
57
DICTYSLUG
Sep-97
Sep-97
3164
25
16
ECOLI
3.5-10
Aug-95
Jan-98
2364
206
180
4-5
Jun-99
Dec-00
449
70
33
4.5-5.5
Oct-98
Dec-00
1025
97
65
5-6
Oct-99
Mar-04
1536
106
81
5.5-6.7
Dec-00
Dec-00
605
16
16
6-9
Dec-00
Dec-00
1100
2
2
6-11
Dec-00
Dec-00
1237
34
31
DIGE 4.5-6.5
May-03
Mar-04
858
174
153
HUMAN
CEC
Jan-98
Mar-04
3077
56
40
CSF
Aug-93
Mar-04
1664
309
30
DLD1
Jan-99
Mar-04
3440
108
67
ELC
Aug-93
Mar-04
2144
35
19
HEPG2
Aug-93
Mar-04
2862
98
45
HEPG2SP
Aug-93
Mar-04
1734
155
25
HL60
Sep-97
Mar-04
3164
26
17
KIDNEY
Sep-97
Mar-04
2896
44
28
LIVER
Aug-93
Mar-04
2413
138
68
NUCLEI LIVER
Apr-00
Mar-04
1497
27
14
LYMPHOCYTE
Mar-04
Mar-04
926
77
62
LYMPHOMA
Aug-93
Mar-04
1890
60
33
NUCLEOLI HELA 2-D PAGE
Nov-01
Mar-04
1271
51
44
NUCLEOLI HELA SDS-PAGE
Nov-01
Mar-04
115
98
189
PLASMA
Aug-93
Mar-04
1966
626
70
PLATELET
Aug-93
Mar-04
2193
41
18
RBC
Aug-93
Mar-04
1800
190
33
U937
Aug-93
Mar-04
895
42
32
MOUSE
ISLETS
Sep-98
May-03
2528
63
44
BAT
Dec-00
Dec-00
2242
85
37
WAT
Sep-98
Oct-01
3540
136
76
LIVER
Sep-98
Apr-00
2836
200
107
NUCLEI LIVER
Apr-00
May-03
4000
15
8
MUSCLE
Sep-98
May-03
2650
136
71
STAPHYLOCOCCUS
Mar-04
Mar-04
596
250
198
YEAST
Feb-95
Mar-04
1940
101
62
6. Some relevant statistics
Species distribution of the SWISS-2DPAGE entries (Total: 1265 entries)
Species code
Entry number
Frequency (%)
Arabidopsis thaliana
57
4.5
Dictyostelium discoideum
16
1.3
Escherichia coli
336
26.6
Homo sapiens
420
33.2
Mus musculus
176
13.9
Staphylococcus aureus
198
15.7
Saccharomyces cerevisiae
62
4.9
Identification methods distribution of the identified spots (Total: 3971 spots)
Identification method
Spot number
Frequency (%)
Amino acids composition
180
4.5
Co-migration
88
2.2
Gel matching
1579
39.8
Immunoblotting
657
16.5
Microsequencing
524
13.2
Peptide mass fingerprinting
1094
27.5
Tandem mass spectrometry
741
18.7
Total exceeds 100% as some of the spots have been identified by several methods.