Protein identification (from 1-DE, 2-DE gels and liquid)
The heart of this new approach is the simultaneous separation of thousand of polypeptides using either electrophoresis or liquid chromatography techniques their further identification combining high-throughput mass spectrometry analysis to powerful bioinformatic tools. The Geneva Proteomics Core Facility provides 3 identification services to which you may send your samples for analysis. These services include tryptic digestion from 1-DE, 2-DE and liquid samples. The peptide mixtures can be then analysed by two alternative workflows: Maldi Tof-Tof or NanoLC - ESI-MS/MS.
Sample preparation
- Gel: The sample preparation, gel separation and gel staining have to be performed according to the SWISS-2DPAGE protocols. Put your gel piece into an Eppendorf tube in 50µl of ethanol 10% and identify your sample with a water-resistant pencil.
- Liquid: For liquid samples Contact us to know how to prepare the samples. Put your liquid sample into an Eppendorf tube and clearly identify your sample with a water-resistant pencil.
Prices
Method | Swiss Fr. / sample 1-10 samples | Swiss Fr. / sample > 10 samples |
Maldi Tof-Tof | 125.00 | 100.00 |
NanoLC - ESI-MS/MS | 300.00 | 250.00 |
Indicated prices takes into account that you are coming from Faculty of Medecine, Uni Geneve or HUG
Maldi Tof-Tof | 150.00 | Inquire |
NanoLC - ESI-MS/MS | 400.00 | Inquire |
Prices do not include VAT (7.6%).
Time delay
Allow three weeks for the delivery of results.