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Protein list for SWISS-2DPAGE ECOLI-DIGE4.5-6.5 
Escherichia coli DIGE (4.5-6.5) 


(map ID: f873b297-1839-4af7-8b27-d6470715ce4b)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for ecoli-dige4.5-6.5   174 identified spots / 153 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods Comment Topics References
aceE RecName: Full=Pyruvate dehydrogenase E1 component; EC=1.2.4.1; P0AFG8
(ODP1_ECOLI)
2D-001WBM
5.39
94'195
{PMF}
Expression
1
aceF RecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; EC=2.3.1.12; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=E2; P06959
(ODP2_ECOLI)
2D-001WDC
5.09
78'105
{PMF}
Expression
1
ackA RecName: Full=Acetate kinase; EC=2.7.2.1; AltName: Full=Acetokinase; P0A6A3
(ACKA_ECOLI)
2D-001WMA
5.79
41'417
{PMF,MS/MS}
-
1
acnB RecName: Full=Aconitate hydratase 2; Short=Aconitase; EC=4.2.1.3; AltName: Full=2-methylisocitrate dehydratase; EC=4.2.1.99; AltName: Full=Citrate hydro-lyase; P36683
(ACON2_ECOLI)
2D-001WBD
5.30
95'000
{PMF}
-
1
ade RecName: Full=Adenine deaminase; Short=Adenase; Short=Adenine aminase; EC=3.5.4.2; P31441
(ADEC_ECOLI)
2D-001WFZ
5.22
61'539
{MS/MS}
Expression
1
ahpC RecName: Full=Alkyl hydroperoxide reductase subunit C; EC=1.11.1.15; AltName: Full=Alkyl hydroperoxide reductase protein C22; AltName: Full=Peroxiredoxin; AltName: Full=SCRP-23; AltName: Full=Sulfate starvation-induced protein 8; Short=SSI8; AltName: Full=Thioredoxin peroxidase; P0AE08
(AHPC_ECOLI)
2D-001WUX
5.09
23'123
{PMF}
-
1
ahpF RecName: Full=Alkyl hydroperoxide reductase subunit F; EC=1.8.1.-; AltName: Full=Alkyl hydroperoxide reductase F52A protein; P35340
(AHPF_ECOLI)
2D-001WHP
5.44
53'485
{MS/MS}
Expression
1
aldA RecName: Full=Lactaldehyde dehydrogenase; EC=1.2.1.22; AltName: Full=Aldehyde dehydrogenase A; AltName: Full=Glycolaldehyde dehydrogenase; EC=1.2.1.21; P25553
(ALDA_ECOLI)
2D-001WIC
5.11
52'363
{MS/MS}
-
1
apt RecName: Full=Adenine phosphoribosyltransferase; Short=APRT; EC=2.4.2.7; P69503
(APT_ECOLI)
2D-001WUK
5.30
23'792
{PMF,MS/MS}
-
1
arcA RecName: Full=Aerobic respiration control protein ArcA; AltName: Full=Dye resistance protein; P0A9Q1
(ARCA_ECOLI)
2D-001WRI
4.52
30'891
{MS/MS}
-
1
argD RecName: Full=Acetylornithine/succinyldiaminopimelate aminotransferase; Short=ACOAT; Short=DapATase; Short=Succinyldiaminopimelate transferase; EC=2.6.1.11; EC=2.6.1.17; P18335
(ARGD_ECOLI)
2D-001WMD
5.75
41'241
{PMF,MS/MS}
-
1
argG RecName: Full=Argininosuccinate synthase; EC=6.3.4.5; AltName: Full=Citrulline--aspartate ligase; P0A6E4
(ASSY_ECOLI)
2D-001WK2
5.27
46'667
{PMF,MS/MS}
-
1
argT RecName: Full=Lysine-arginine-ornithine-binding periplasmic protein; Short=LAO-binding protein; Flags: Precursor; P09551
(ARGT_ECOLI)
2D-001WT8
5.15
26'055
{MS/MS}
Expression
1
aroF RecName: Full=Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive; EC=2.5.1.54; AltName: Full=3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; AltName: Full=DAHP synthase; AltName: Full=Phospho-2-keto-3-deoxyheptonate aldolase; P00888
(AROF_ECOLI)
2D-001WNR
5.38
37'715
{MS/MS}
-
1
aroK RecName: Full=Shikimate kinase 1; Short=SK 1; EC=2.7.1.71; AltName: Full=Shikimate kinase I; Short=SKI; P0A6D7
(AROK_ECOLI)
2D-001WWI
5.30
19'078
{PMF}
-
1
artI RecName: Full=Putative ABC transporter arginine-binding protein 2; Flags: Precursor; P30859
(ARTI_ECOLI)
2D-001WTV
5.27
24'964
{PMF,MS/MS}
Expression
1
artJ RecName: Full=ABC transporter arginine-binding protein 1; Flags: Precursor; P30860
(ARTJ_ECOLI)
2D-001WTW
2D-001WU5
5.94
5.59
25'009
24'831
{PMF}
{MS/MS}
Expression
Expression
1
1
asnA RecName: Full=Aspartate--ammonia ligase; EC=6.3.1.1; AltName: Full=Asparagine synthetase A; P00963
(ASNA_ECOLI)
2D-001WNR
2D-001WNK
5.38
5.45
37'715
37'876
{MS/MS}
{MS/MS}
Expression
Expression
1
1
asnS RecName: Full=Asparaginyl-tRNA synthetase; EC=6.1.1.22; AltName: Full=Asparagine--tRNA ligase; Short=AsnRS; P0A8M0
(SYN_ECOLI)
2D-001WIT
4.69
50'800
{MS/MS}
-
1
atpA RecName: Full=ATP synthase subunit alpha; EC=3.6.3.14; AltName: Full=ATP synthase F1 sector subunit alpha; AltName: Full=F-ATPase subunit alpha; P0ABB0
(ATPA_ECOLI)
2D-001WIG
2D-001WMM
5.82
5.15
52'204
39'861
{PMF}
{MS/MS, PMF}
-
-
1
1
atpD RecName: Full=ATP synthase subunit beta; EC=3.6.3.14; AltName: Full=ATP synthase F1 sector subunit beta; AltName: Full=F-ATPase subunit beta; P0ABB4
(ATPB_ECOLI)
2D-001WJO
2D-001WJK
4.96
4.99
48'103
48'103
{PMF}
{MS/MS, PMF}
Expression
Expression
1
1
atpF RecName: Full=ATP synthase subunit b; AltName: Full=ATP synthase F(0) sector subunit b; AltName: Full=ATPase subunit I; AltName: Full=F-type ATPase subunit b; Short=F-ATPase subunit b; P0ABA0
(ATPF_ECOLI)
2D-001WWG
2D-001WWO
5.53
5.53
19'289
18'722
{PMF}
{MS/MS, PMF}
Expression
Expression
1
1
bcp RecName: Full=Putative peroxiredoxin bcp; EC=1.11.1.15; AltName: Full=Bacterioferritin comigratory protein; AltName: Full=Thioredoxin reductase; P0AE52
(BCP_ECOLI)
2D-001WWV
5.10
17'526
{MS/MS}
-
1
carA RecName: Full=Carbamoyl-phosphate synthase small chain; EC=6.3.5.5; AltName: Full=Carbamoyl-phosphate synthetase glutamine chain; P0A6F1
(CARA_ECOLI)
2D-001WM3
2D-001WMA
2D-001WM0
5.82
5.79
5.88
42'128
41'417
42'128
{MS/MS}
{MS/MS}
{MS/MS, PMF}
-
-
-
1
1
1
cdd RecName: Full=Cytidine deaminase; EC=3.5.4.5; AltName: Full=Cytidine aminohydrolase; Short=CDA; P0ABF6
(CDD_ECOLI)
2D-001WQP
5.36
32'716
{MS/MS}
-
1
cirA RecName: Full=Colicin I receptor; Flags: Precursor; P17315
(CIRA_ECOLI)
2D-001WE6
5.06
73'587
{MS/MS}
Expression
1
clpB RecName: Full=Chaperone protein ClpB; AltName: Full=Heat shock protein F84.1; P63284
(CLPB_ECOLI)
2D-001WC2
5.33
88'745
{PMF,MS/MS}
Expression
1
cysK RecName: Full=Cysteine synthase A; Short=CSase A; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase A; Short=OAS-TL A; AltName: Full=O-acetylserine sulfhydrylase A; AltName: Full=Sulfate starvation-induced protein 5; Short=SSI5; P0ABK5
(CYSK_ECOLI)
2D-001WPI
5.83
34'342
{PMF,MS/MS}
-
1
dapB RecName: Full=Dihydrodipicolinate reductase; Short=DHPR; EC=1.3.1.26; P04036
(DAPB_ECOLI)
2D-001WS5
5.37
27'969
{PMF}
Expression
1
dapD RecName: Full=2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase; EC=2.3.1.117; AltName: Full=Tetrahydrodipicolinate N-succinyltransferase; Short=THDP succinyltransferase; Short=THP succinyltransferase; Short=Tetrahydropicolinate succinylase; P0A9D8
(DAPD_ECOLI)
2D-001WQW
5.49
32'476
{PMF,MS/MS}
-
1
dcrB RecName: Full=Protein dcrB; Flags: Precursor; P0AEE1
(DCRB_ECOLI)
2D-001WWQ
4.50
18'143
{MS/MS}
Expression
1
deaD RecName: Full=Cold-shock DEAD box protein A; EC=3.6.4.13; AltName: Full=ATP-dependent RNA helicase deaD; AltName: Full=Translation factor W2; P0A9P6
(DEAD_ECOLI)
2D-001WO0
5.41
37'079
{MS/MS}
Expression
1
deoD RecName: Full=Purine nucleoside phosphorylase deoD-type; Short=PNP; EC=2.4.2.1; AltName: Full=Inosine phosphorylase; P0ABP8
(DEOD_ECOLI)
2D-001WTS
5.40
25'098
{MS/MS}
Expression
1
dhaM RecName: Full=PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM; Includes: RecName: Full=Phosphocarrier protein HPr; Short=Protein H; Includes: RecName: Full=Phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=PTS system EIIA component; P37349
(DHAM_ECOLI)
2D-001WIT
4.69
50'800
{MS/MS}
-
1
dksA RecName: Full=DnaK suppressor protein; P0ABS1
(DKSA_ECOLI)
2D-001WWC
5.08
19'656
{MS/MS}
-
1
dnaK RecName: Full=Chaperone protein DnaK; AltName: Full=HSP70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=Heat shock protein 70; P0A6Y8
(DNAK_ECOLI)
2D-001WE2
2D-001WDY
2D-001WDV
4.94
4.91
4.92
73'587
74'850
74'532
{PMF}
{MS/MS}
{PMF}
Expression
Expression
Expression
1
1
1
dps RecName: Full=DNA protection during starvation protein; EC=1.16.-.-; P0ABT2
(DPS_ECOLI)
2D-001WWO
5.53
18'722
{MS/MS}
-
1
dsbA RecName: Full=Thiol:disulfide interchange protein DsbA; Flags: Precursor; P0AEG4
(DSBA_ECOLI)
2D-001WVA
5.34
22'606
{PMF,MS/MS}
-
1
dut RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase; Short=dUTPase; EC=3.6.1.23; AltName: Full=dUTP pyrophosphatase; P06968
(DUT_ECOLI)
2D-001WWV
5.10
17'526
{MS/MS}
-
1
eda RecName: Full=KHG/KDPG aldolase; Includes: RecName: Full=4-hydroxy-2-oxoglutarate aldolase; EC=4.1.3.16; AltName: Full=2-keto-4-hydroxyglutarate aldolase; Short=KHG-aldolase; Includes: RecName: Full=2-dehydro-3-deoxy-phosphogluconate aldolase; EC=4.1.2.14; AltName: Full=2-keto-3-deoxy-6-phosphogluconate aldolase; Short=KDPG-aldolase; AltName: Full=Phospho-2-dehydro-3-deoxygluconate aldolase; AltName: Full=Phospho-2-keto-3-deoxygluconate aldolase; P0A955
(ALKH_ECOLI)
2D-001WUW
4.93
23'247
{PMF}
Expression
1
engD RecName: Full=GTP-dependent nucleic acid-binding protein engD; P0ABU2
(ENGD_ECOLI)
2D-001WLW
5.03
42'217
{PMF}
-
1
eno RecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase; P0A6P9
(ENO_ECOLI)
2D-001WL3
5.35
43'401
{PMF,MS/MS}
-
1
entB RecName: Full=Isochorismatase; EC=3.3.2.1; AltName: Full=2,3 dihydro-2,3 dihydroxybenzoate synthase; AltName: Full=Enterobactin synthase component B; AltName: Full=Enterochelin synthase B; AltName: Full=Isochorismate lyase; P0ADI4
(ENTB_ECOLI)
2D-001WPF
5.08
34'633
{MS/MS}
-
1
fabD RecName: Full=Malonyl CoA-acyl carrier protein transacylase; Short=MCT; EC=2.3.1.39; P0AAI9
(FABD_ECOLI)
2D-001WQ5
5.03
33'449
{PMF,MS/MS}
-
1
fabI RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] FabI; Short=ENR; EC=1.3.1.9; AltName: Full=NADH-dependent enoyl-ACP reductase; P0AEK4
(FABI_ECOLI)
2D-001WQG
2D-001WQS
5.40
5.60
32'959
32'613
{MS/MS}
{MS/MS, PMF}
Expression
Expression
1
1
fbaA RecName: Full=Fructose-bisphosphate aldolase class 2; Short=FBP aldolase; Short=FBPA; EC=4.1.2.13; AltName: Full=Fructose-1,6-bisphosphate aldolase; AltName: Full=Fructose-bisphosphate aldolase class II; P0AB71
(ALF_ECOLI)
2D-001WMY
5.49
39'104
{PMF}
-
1
fepA RecName: Full=Ferrienterobactin receptor; AltName: Full=Enterobactin outer-membrane receptor; Flags: Precursor; P05825
(FEPA_ECOLI)
2D-001WD7
5.24
79'447
{MS/MS}
Expression
1
fklB RecName: Full=FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase; Short=FKBP22; Short=PPIase; EC=5.2.1.8; AltName: Full=Rotamase; P0A9L3
(FKBB_ECOLI)
2D-001WUA
4.84
24'436
{MS/MS}
-
1
fliY RecName: Full=Cystine-binding periplasmic protein; Short=CBP; AltName: Full=Protein fliY; AltName: Full=Sulfate starvation-induced protein 7; Short=SSI7; Flags: Precursor; P0AEM9
(FLIY_ECOLI)
2D-001WTE
5.17
25'686
{PMF}
-
1
folA RecName: Full=Dihydrofolate reductase; EC=1.5.1.3; P0ABQ4
(DYR_ECOLI)
2D-001WVR
5.02
21'096
{MS/MS}
Expression
1
fruB RecName: Full=Multiphosphoryl transfer protein; Short=MTP; AltName: Full=Phosphotransferase FPr protein; AltName: Full=Pseudo-HPr; Includes: RecName: Full=Phosphocarrier protein HPr; Short=Protein H; Includes: RecName: Full=Fructose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=PTS system fructose-specific EIIA component; P69811
(PTFAH_ECOLI)
2D-001WN5
4.85
38'938
{MS/MS}
Expression
1
fusA RecName: Full=Elongation factor G; Short=EF-G; P0A6M8
(EFG_ECOLI)
2D-001WCV
2D-001WCU
2D-001WN8
2D-001WR8
2D-001WK8
5.20
5.29
5.20
5.78
5.51
84'325
85'046
38'691
32'101
46'525
{PMF}
{PMF}
{MS/MS}
{MS/MS}
{MS/MS}
-
-
-
-
-
1
1
1
1
1
galE RecName: Full=UDP-glucose 4-epimerase; EC=5.1.3.2; AltName: Full=Galactowaldenase; AltName: Full=UDP-galactose 4-epimerase; P09147
(GALE_ECOLI)
2D-001WOQ
5.91
35'787
{MS/MS}
Expression
1
gapA RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A; Short=GAPDH-A; EC=1.2.1.12; P0A9B2
(G3P1_ECOLI)
2D-001WOM
2D-001WVB
5.94
5.94
36'106
22'606
{MS/MS}
{PMF}
-
-
1
1
gdhA RecName: Full=NADP-specific glutamate dehydrogenase; Short=NADP-GDH; EC=1.4.1.4; P00370
(DHE4_ECOLI)
2D-001WKM
2D-001WK7
5.94
5.81
44'524
46'385
{MS/MS, PMF}
{PMF}
-
-
1
1
glnA RecName: Full=Glutamine synthetase; EC=6.3.1.2; AltName: Full=Glutamate--ammonia ligase; P0A9C5
(GLNA_ECOLI)
2D-001WHR
5.30
53'162
{MS/MS}
Expression
1
glnS RecName: Full=Glutaminyl-tRNA synthetase; EC=6.1.1.18; AltName: Full=Glutamine--tRNA ligase; Short=GlnRS; P00962
(SYQ_ECOLI)
2D-001WE0
5.91
74'850
{MS/MS}
-
1
glyA RecName: Full=Serine hydroxymethyltransferase; Short=SHMT; Short=Serine methylase; EC=2.1.2.1; P0A825
(GLYA_ECOLI)
2D-001WKJ
2D-001WLG
2D-001WKH
6.00
6.00
6.02
45'000
43'217
45'274
{MS/MS, PMF}
{MS/MS}
{MS/MS, PMF}
Expression
Expression
Expression
1
1
1
gph RecName: Full=Phosphoglycolate phosphatase; Short=PGP; Short=PGPase; EC=3.1.3.18; P32662
(GPH_ECOLI)
2D-001WRI
4.52
30'891
{MS/MS}
-
1
gpmA RecName: Full=2,3-bisphosphoglycerate-dependent phosphoglycerate mutase; Short=BPG-dependent PGAM; Short=PGAM; Short=Phosphoglyceromutase; Short=dPGM; EC=5.4.2.1; P62707
(GPMA_ECOLI)
2D-001WSL
5.86
27'000
{PMF}
-
1
gpmI RecName: Full=2,3-bisphosphoglycerate-independent phosphoglycerate mutase; Short=BPG-independent PGAM; Short=Phosphoglyceromutase; Short=iPGM; EC=5.4.2.1; P37689
(GPMI_ECOLI)
2D-001WIC
2D-001WI2
5.11
5.15
52'363
52'681
{MS/MS}
{MS/MS, PMF}
-
-
1
1
groL RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60; P0A6F5
(CH60_ECOLI)
2D-001WG2
2D-001WFW
2D-001WFT
4.98
4.96
4.93
60'758
59'987
61'278
{PMF}
{MS/MS, PMF}
{PMF}
-
-
-
1
1
1
groS RecName: Full=10 kDa chaperonin; AltName: Full=GroES protein; AltName: Full=Protein Cpn10; P0A6F9
(CH10_ECOLI)
2D-001WXP
5.15
10'500
{MS/MS}
-
1
guaB RecName: Full=Inosine-5'-monophosphate dehydrogenase; Short=IMP dehydrogenase; Short=IMPD; Short=IMPDH; EC=1.1.1.205; P0ADG7
(IMDH_ECOLI)
2D-001WH8
5.99
55'298
{PMF,MS/MS}
-
1
hchA RecName: Full=Chaperone protein hchA; AltName: Full=EcHsp31; AltName: Full=Hsp31; P31658
(HCHA_ECOLI)
2D-001WQA
5.44
33'098
{MS/MS}
-
1
hemB RecName: Full=Delta-aminolevulinic acid dehydratase; Short=ALAD; Short=ALADH; EC=4.2.1.24; AltName: Full=Porphobilinogen synthase; P0ACB2
(HEM2_ECOLI)
2D-001WN8
5.20
38'691
{MS/MS}
-
1
hisA RecName: Full=1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; EC=5.3.1.16; AltName: Full=Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; P10371
(HIS4_ECOLI)
2D-001WUQ
2D-001WU7
5.46
4.99
23'581
24'831
{PMF}
{PMF}
-
-
1
1
hisD RecName: Full=Histidinol dehydrogenase; Short=HDH; EC=1.1.1.23; P06988
(HISX_ECOLI)
2D-001WJG
5.07
48'837
{PMF}
-
1
hisG RecName: Full=ATP phosphoribosyltransferase; Short=ATP-PRT; Short=ATP-PRTase; EC=2.4.2.17; P60757
(HIS1_ECOLI)
2D-001WQG
5.40
32'959
{PMF,MS/MS}
-
1
hisJ RecName: Full=Histidine-binding periplasmic protein; Short=HBP; Flags: Precursor; P0AEU0
(HISJ_ECOLI)
2D-001WS8
2D-001WSD
5.28
5.14
27'969
27'701
{PMF}
{PMF}
-
-
1
1
hldD RecName: Full=ADP-L-glycero-D-manno-heptose-6-epimerase; EC=5.1.3.20; AltName: Full=ADP-L-glycero-beta-D-manno-heptose-6-epimerase; Short=ADP-glyceromanno-heptose 6-epimerase; Short=ADP-hep 6-epimerase; Short=AGME; P67910
(HLDD_ECOLI)
2D-001WOJ
4.94
36'106
{PMF}
-
1
icd RecName: Full=Isocitrate dehydrogenase [NADP]; Short=IDH; EC=1.1.1.42; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase; P08200
(IDH_ECOLI)
2D-001WKP
2D-001WKS
5.11
5.15
44'335
44'053
{PMF}
{PMF}
Expression
Expression
1
1
ilvC RecName: Full=Ketol-acid reductoisomerase; EC=1.1.1.86; AltName: Full=Acetohydroxy-acid isomeroreductase; AltName: Full=Alpha-keto-beta-hydroxylacil reductoisomerase; P05793
(ILVC_ECOLI)
2D-001WIH
2D-001WIK
5.22
5.29
52'046
51'264
{MS/MS}
{MS/MS}
Expression
Expression
1
1
ilvD RecName: Full=Dihydroxy-acid dehydratase; Short=DAD; EC=4.2.1.9; P05791
(ILVD_ECOLI)
2D-001WFQ
5.54
62'065
{PMF,MS/MS}
Expression
1
katE RecName: Full=Catalase HPII; EC=1.11.1.6; AltName: Full=Hydroxyperoxidase II; P21179
(CATE_ECOLI)
2D-001WCG
5.47
86'507
{PMF}
Expression
1
katG RecName: Full=Catalase-peroxidase; Short=CP; EC=1.11.1.21; AltName: Full=Hydroperoxidase I; Short=HPI; AltName: Full=Peroxidase/catalase; P13029
(KATG_ECOLI)
2D-001WDM
5.18
77'774
{MS/MS}
Expression
1
leuC RecName: Full=3-isopropylmalate dehydratase large subunit; EC=4.2.1.33; AltName: Full=Alpha-IPM isomerase; Short=IPMI; AltName: Full=Isopropylmalate isomerase; P0A6A6
(LEUC_ECOLI)
2D-001WIX
5.93
50'340
{PMF}
Expression
1
leuD RecName: Full=3-isopropylmalate dehydratase small subunit; EC=4.2.1.33; AltName: Full=Alpha-IPM isomerase; Short=IPMI; AltName: Full=Isopropylmalate isomerase; P30126
(LEUD_ECOLI)
2D-001WV1
5.20
23'000
{PMF,MS/MS}
Expression
1
livJ RecName: Full=Leu/Ile/Val-binding protein; Short=LIV-BP; Flags: Precursor; P0AD96
(LIVJ_ECOLI)
2D-001WN2
2D-001WMS
5.30
5.18
38'773
39'439
{PMF,MS/MS}
{PMF,MS/MS}
Expression
Expression
1
1
livK RecName: Full=Leucine-specific-binding protein; Short=L-BP; Short=LS-BP; Flags: Precursor; P04816
(LIVK_ECOLI)
2D-001WMV
5.09
39'439
{PMF,MS/MS}
Expression
1
lpdA RecName: Full=Dihydrolipoyl dehydrogenase; EC=1.8.1.4; AltName: Full=Dihydrolipoamide dehydrogenase; AltName: Full=E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; AltName: Full=Glycine cleavage system L protein; P0A9P0
(DLDH_ECOLI)
2D-001WIU
2D-001WS6
5.82
5.90
50'800
28'059
{PMF}
{MS/MS, PMF}
-
-
1
1
lptD RecName: Full=LPS-assembly protein lptD; AltName: Full=Organic solvent tolerance protein; Flags: Precursor; P31554
(LPTD_ECOLI)
2D-001WC1
4.91
88'368
{PMF}
-
1
malE RecName: Full=Maltose-binding periplasmic protein; AltName: Full=MBP; AltName: Full=MMBP; AltName: Full=Maltodextrin-binding protein; Flags: Precursor; P0AEX9
(MALE_ECOLI)
2D-001WN8
5.20
38'691
{MS/MS}
-
1
mdh RecName: Full=Malate dehydrogenase; EC=1.1.1.37; P61889
(MDH_ECOLI)
2D-001WOZ
5.56
35'156
{PMF}
-
1
metQ RecName: Full=D-methionine-binding lipoprotein metQ; Flags: Precursor; P28635
(METQ_ECOLI)
2D-001WSM
4.90
27'000
{PMF,MS/MS}
Expression
1
nemA RecName: Full=N-ethylmaleimide reductase; EC=1.-.-.-; AltName: Full=N-ethylmaleimide reducing enzyme; P77258
(NEMA_ECOLI)
2D-001WN7
5.78
39'021
{MS/MS}
-
1
nfnB RecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; EC=1.-.-.-; AltName: Full=Dihydropteridine reductase; EC=1.5.1.34; AltName: Full=FMN-dependent nitroreductase; P38489
(NFNB_ECOLI)
2D-001WU3
5.83
24'964
{PMF,MS/MS}
-
1
nlpA RecName: Full=Lipoprotein 28; Flags: Precursor; P04846
(NLPA_ECOLI)
2D-001WWT
5.15
17'637
{PMF,MS/MS}
-
1
nlpB RecName: Full=Lipoprotein 34; Flags: Precursor; P0A903
(NLPB_ECOLI)
2D-001WP0
4.88
35'000
{MS/MS}
-
1
nuoB RecName: Full=NADH-quinone oxidoreductase subunit B; EC=1.6.99.5; AltName: Full=NADH dehydrogenase I subunit B; AltName: Full=NDH-1 subunit B; AltName: Full=NUO2; P0AFC7
(NUOB_ECOLI)
2D-001WTM
5.46
25'413
{MS/MS}
-
1
ompA RecName: Full=Outer membrane protein A; AltName: Full=Outer membrane protein II*; Flags: Precursor; P0A910
(OMPA_ECOLI)
2D-001WPP
2D-001WPU
5.50
5.38
33'875
33'875
{PMF}
{MS/MS}
Expression
Expression
1
1
ompF RecName: Full=Outer membrane protein F; AltName: Full=Outer membrane protein 1A; AltName: Full=Outer membrane protein B; AltName: Full=Outer membrane protein IA; AltName: Full=Porin OmpF; Flags: Precursor; P02931
(OMPF_ECOLI)
2D-001WO6
2D-001WNX
2D-001WNW
4.71
4.64
4.56
37'000
37'000
37'316
{MS/MS}
{MS/MS}
{MS/MS}
Expression
Expression
Expression
1
1
1
ompT RecName: Full=Protease 7; EC=3.4.23.49; AltName: Full=Omptin; AltName: Full=Outer membrane protein 3B; AltName: Full=Protease A; AltName: Full=Protease VII; Flags: Precursor; P09169
(OMPT_ECOLI)
2D-001WO5
5.36
37'000
{PMF,MS/MS}
-
1
ompX RecName: Full=Outer membrane protein X; Flags: Precursor; P0A917
(OMPX_ECOLI)
2D-001WXD
2D-001WX9
5.15
5.25
16'000
16'126
{MS/MS}
{MS/MS}
-
-
1
1
oppA RecName: Full=Periplasmic oligopeptide-binding protein; Flags: Precursor; P23843
(OPPA_ECOLI)
2D-001WI9
2D-001WH6
2D-001WHC
5.71
5.93
5.82
52'681
55'635
55'131
{PMF}
{PMF}
{PMF}
Expression
Expression
Expression
1
1
1
otsB RecName: Full=Trehalose-phosphate phosphatase; Short=TPP; EC=3.1.3.12; AltName: Full=Osmoregulatory trehalose synthesis protein B; AltName: Full=Trehalose 6-phosphate phosphatase; AltName: Full=Trehalose-phosphatase; P31678
(OTSB_ECOLI)
2D-001WRR
5.32
29'821
{MS/MS}
-
1
panC RecName: Full=Pantothenate synthetase; Short=PS; EC=6.3.2.1; AltName: Full=Pantoate--beta-alanine ligase; AltName: Full=Pantoate-activating enzyme; P31663
(PANC_ECOLI)
2D-001WR0
5.63
32'442
{MS/MS}
-
1
pepD RecName: Full=Cytosol non-specific dipeptidase; EC=3.4.13.18; AltName: Full=Aminoacyl-histidine dipeptidase; AltName: Full=Beta-alanyl-histidine dipeptidase; AltName: Full=Carnosinase; AltName: Full=Cysteinylglycinase; AltName: Full=Peptidase D; AltName: Full=Xaa-His dipeptidase; Short=X-His dipeptidase; P15288
(PEPD_ECOLI)
2D-001WIH
5.22
52'046
{MS/MS}
-
1
pepQ RecName: Full=Xaa-Pro dipeptidase; Short=X-Pro dipeptidase; EC=3.4.13.9; AltName: Full=Imidodipeptidase; AltName: Full=Proline dipeptidase; Short=Prolidase; P21165
(PEPQ_ECOLI)
2D-001WJ2
5.62
50'340
{PMF,MS/MS}
Expression
1
pfkA RecName: Full=6-phosphofructokinase isozyme 1; EC=2.7.1.11; AltName: Full=6-phosphofructokinase isozyme I; AltName: Full=Phosphofructokinase 1; AltName: Full=Phosphohexokinase 1; P0A796
(K6PF1_ECOLI)
2D-001WOI
5.43
36'348
{PMF}
-
1
pgk RecName: Full=Phosphoglycerate kinase; EC=2.7.2.3; P0A799
(PGK_ECOLI)
2D-001WI6
5.30
52'681
{PMF,MS/MS}
-
1
pnp RecName: Full=Polyribonucleotide nucleotidyltransferase; EC=2.7.7.8; AltName: Full=Polynucleotide phosphorylase; Short=PNPase; P05055
(PNP_ECOLI)
2D-001WDK
2D-001WD2
5.15
5.16
78'105
80'468
{MS/MS}
{PMF}
Expression
Expression
1
1
potD RecName: Full=Spermidine/putrescine-binding periplasmic protein; Short=SPBP; Flags: Precursor; P0AFK9
(POTD_ECOLI)
2D-001WP0
4.88
35'000
{MS/MS}
-
1
potF RecName: Full=Putrescine-binding periplasmic protein; Flags: Precursor; P31133
(POTF_ECOLI)
2D-001WOL
5.48
36'267
{PMF}
-
1
ppa RecName: Full=Inorganic pyrophosphatase; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase; Short=PPase; P0A7A9
(IPYR_ECOLI)
2D-001WV8
5.00
22'748
{PMF}
-
1
proX RecName: Full=Glycine betaine-binding periplasmic protein; Flags: Precursor; P0AFM2
(PROX_ECOLI)
2D-001WQA
2D-001WQ7
5.44
5.65
33'098
33'203
{MS/MS}
{MS/MS}
-
-
1
1
ptsI RecName: Full=Phosphoenolpyruvate-protein phosphotransferase; EC=2.7.3.9; AltName: Full=Phosphotransferase system, enzyme I; P08839
(PT1_ECOLI)
2D-001WFL
4.91
63'671
{PMF,MS/MS}
Expression
1
purA RecName: Full=Adenylosuccinate synthetase; Short=AMPSase; Short=AdSS; EC=6.3.4.4; AltName: Full=IMP--aspartate ligase; P0A7D4
(PURA_ECOLI)
2D-001WKQ
2D-001WLA
5.30
5.30
44'524
43'494
{PMF}
{MS/MS}
Expression
Expression
1
1
purC RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase; EC=6.3.2.6; AltName: Full=SAICAR synthetase; P0A7D7
(PUR7_ECOLI)
2D-001WT3
5.09
26'148
{PMF}
Expression
1
purD RecName: Full=Phosphoribosylamine--glycine ligase; EC=6.3.4.13; AltName: Full=GARS; AltName: Full=Glycinamide ribonucleotide synthetase; AltName: Full=Phosphoribosylglycinamide synthetase; P15640
(PUR2_ECOLI)
2D-001WJS
5.06
47'667
{PMF}
Expression
1
purE RecName: Full=N5-carboxyaminoimidazole ribonucleotide mutase; Short=N5-CAIR mutase; EC=5.4.99.18; AltName: Full=5-(carboxyamino)imidazole ribonucleotide mutase; P0AG18
(PURE_ECOLI)
2D-001WWL
6.05
18'899
{MS/MS}
-
1
purH RecName: Full=Bifunctional purine biosynthesis protein PurH; Includes: RecName: Full=Phosphoribosylaminoimidazolecarboxamide formyltransferase; EC=2.1.2.3; AltName: Full=AICAR transformylase; Includes: RecName: Full=IMP cyclohydrolase; EC=3.5.4.10; AltName: Full=ATIC; AltName: Full=IMP synthase; AltName: Full=Inosinicase; P15639
(PUR9_ECOLI)
2D-001WGU
5.50
57'000
{PMF}
Expression
1
pykF RecName: Full=Pyruvate kinase I; EC=2.7.1.40; AltName: Full=PK-1; P0AD61
(KPYK1_ECOLI)
2D-001WH2
5.65
56'143
{PMF}
-
1
pyrF RecName: Full=Orotidine 5'-phosphate decarboxylase; EC=4.1.1.23; AltName: Full=OMP decarboxylase; Short=OMPDCase; Short=OMPdecase; P08244
(PYRF_ECOLI)
2D-001WU3
5.83
24'964
{MS/MS}
-
1
pyrG RecName: Full=CTP synthase; EC=6.3.4.2; AltName: Full=CTP synthetase; AltName: Full=UTP--ammonia ligase; P0A7E5
(PYRG_ECOLI)
2D-001WGG
5.61
58'475
{PMF}
-
1
rbsB RecName: Full=D-ribose-binding periplasmic protein; Flags: Precursor; P02925
(RBSB_ECOLI)
2D-001WRY
5.92
29'066
{PMF,MS/MS}
-
1
ribH RecName: Full=6,7-dimethyl-8-ribityllumazine synthase; Short=DMRL synthase; Short=Lumazine synthase; EC=2.5.1.9; AltName: Full=Riboflavin synthase beta chain; P61714
(RISB_ECOLI)
2D-001WXK
5.15
16'000
{MS/MS}
Expression
1
rimM RecName: Full=Ribosome maturation factor rimM; AltName: Full=21K; P0A7X6
(RIMM_ECOLI)
2D-001WUC
4.68
24'436
{MS/MS}
Expression
1
rmlA1 RecName: Full=Glucose-1-phosphate thymidylyltransferase 1; Short=G1P-TT 1; EC=2.7.7.24; AltName: Full=dTDP-glucose pyrophosphorylase 1; AltName: Full=dTDP-glucose synthase 1; P37744
(RMLA1_ECOLI)
2D-001WQU
5.41
32'544
{MS/MS}
Expression
1
rpoA RecName: Full=DNA-directed RNA polymerase subunit alpha; Short=RNAP subunit alpha; EC=2.7.7.6; AltName: Full=RNA polymerase subunit alpha; AltName: Full=Transcriptase subunit alpha; P0A7Z4
(RPOA_ECOLI)
2D-001WMR
2D-001WMU
5.03
5.07
39'691
39'523
{PMF}
{MS/MS, PMF}
-
-
1
1
rpsA RecName: Full=30S ribosomal protein S1; P0AG67
(RS1_ECOLI)
2D-001WEH
4.97
68'742
{PMF}
-
1
rpsB RecName: Full=30S ribosomal protein S2; P0A7V0
(RS2_ECOLI)
2D-001WWJ
4.83
19'048
{MS/MS}
-
1
rpsF RecName: Full=30S ribosomal protein S6; Contains: RecName: Full=30S ribosomal protein S6, fully modified isoform; Contains: RecName: Full=30S ribosomal protein S6, non-modified isoform; P02358
(RS6_ECOLI)
2D-001WXA
2D-001WXJ
5.30
5.20
16'076
16'000
{PMF,MS/MS}
{PMF,MS/MS}
-
-
1
1
rsuA RecName: Full=Ribosomal small subunit pseudouridine synthase A; EC=5.4.99.19; AltName: Full=16S pseudouridylate 516 synthase; AltName: Full=16S rRNA pseudouridine(516) synthase; AltName: Full=rRNA pseudouridylate synthase A; AltName: Full=rRNA-uridine isomerase A; P0AA43
(RSUA_ECOLI)
2D-001WR0
2D-001WR8
5.63
5.78
32'442
32'101
{MS/MS}
{MS/MS, PMF}
-
-
1
1
serC RecName: Full=Phosphoserine aminotransferase; EC=2.6.1.52; AltName: Full=Phosphohydroxythreonine aminotransferase; Short=PSAT; P23721
(SERC_ECOLI)
2D-001WN9
5.34
38'691
{PMF}
-
1
speB RecName: Full=Agmatinase; EC=3.5.3.11; AltName: Full=Agmatine ureohydrolase; Short=AUH; P60651
(SPEB_ECOLI)
2D-001WR2
5.14
32'339
{MS/MS}
Expression
1
speE RecName: Full=Spermidine synthase; EC=2.5.1.16; AltName: Full=Putrescine aminopropyltransferase; Short=PAPT; AltName: Full=SPDSY; P09158
(SPEE_ECOLI)
2D-001WRB
5.30
32'000
{PMF,MS/MS}
Expression
1
surA RecName: Full=Chaperone surA; AltName: Full=Peptidyl-prolyl cis-trans isomerase surA; Short=PPIase surA; EC=5.2.1.8; AltName: Full=Rotamase surA; AltName: Full=Survival protein A; Flags: Precursor; P0ABZ6
(SURA_ECOLI)
2D-001WKD
5.83
45'411
{PMF}
-
1
talB RecName: Full=Transaldolase B; EC=2.2.1.2; P0A870
(TALB_ECOLI)
2D-001WP1
5.10
35'000
{PMF}
-
1
tesA RecName: Full=Acyl-CoA thioesterase I; EC=3.1.2.-; AltName: Full=Lecithinase B; AltName: Full=Lysophospholipase L1; EC=3.1.1.5; AltName: Full=Protease I; Flags: Precursor; P0ADA1
(TESA_ECOLI)
2D-001WW8
5.97
19'936
{MS/MS}
-
1
thiE RecName: Full=Thiamine-phosphate pyrophosphorylase; Short=TMP pyrophosphorylase; Short=TMP-PPase; EC=2.5.1.3; AltName: Full=Thiamine-phosphate synthase; P30137
(THIE_ECOLI)
2D-001WU0
5.51
24'964
{MS/MS}
-
1
thrB RecName: Full=Homoserine kinase; Short=HK; Short=HSK; EC=2.7.1.39; P00547
(KHSE_ECOLI)
2D-001WR7
5.38
32'000
{PMF,MS/MS}
Expression
1
thrC RecName: Full=Threonine synthase; Short=TS; EC=4.2.3.1; P00934
(THRC_ECOLI)
2D-001WK2
5.27
46'667
{MS/MS}
-
1
tig RecName: Full=Trigger factor; Short=TF; P0A850
(TIG_ECOLI)
2D-001WI0
4.94
52'681
{PMF,MS/MS}
-
1
tnaA RecName: Full=Tryptophanase; EC=4.1.99.1; AltName: Full=L-tryptophan indole-lyase; Short=TNase; P0A853
(TNAA_ECOLI)
2D-001WJL
5.81
48'395
{MS/MS}
Expression
1
tolC RecName: Full=Outer membrane protein tolC; Flags: Precursor; P02930
(TOLC_ECOLI)
2D-001WJ6
5.16
50'036
{PMF}
-
1
tpiA RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase; P0A858
(TPIS_ECOLI)
2D-001WTA
5.51
25'962
{MS/MS}
Expression
1
tpx RecName: Full=Thiol peroxidase; EC=1.11.1.-; AltName: Full=Scavengase P20; P0A862
(TPX_ECOLI)
2D-001WWA
4.99
19'656
{PMF,MS/MS}
Expression
1
trpB RecName: Full=Tryptophan synthase beta chain; EC=4.2.1.20; P0A879
(TRPB_ECOLI)
2D-001WMD
5.75
41'241
{MS/MS}
-
1
tsf RecName: Full=Elongation factor Ts; Short=EF-Ts; P0A6P1
(EFTS_ECOLI)
2D-001WPG
2D-001WPF
2D-001WPB
5.20
5.08
5.14
34'415
34'633
34'596
{MS/MS, PMF}
{MS/MS}
{MS/MS, PMF}
Expression
Expression
Expression
1
1
1
tufB RecName: Full=Elongation factor Tu 2; Short=EF-Tu 2; AltName: Full=P-43; P0CE48
(EFTU2_ECOLI)
2D-001WQY
2D-001WLP
2D-001WLU
2D-001WTU
5.07
5.38
5.40
5.69
32'442
41'860
42'307
25'098
{MS/MS}
{PMF}
{MS/MS, PMF}
{MS/MS}
Expression
Expression
Expression
Expression
1
1
1
1
wrbA RecName: Full=Flavoprotein wrbA; AltName: Full=Trp repressor-binding protein; P0A8G6
(WRBA_ECOLI)
2D-001WUJ
5.48
24'005
{PMF}
-
1
yahK RecName: Full=Uncharacterized zinc-type alcohol dehydrogenase-like protein YahK; EC=1.-.-.-; P75691
(YAHK_ECOLI)
2D-001WM3
5.82
42'128
{PMF,MS/MS}
-
1
ybbN RecName: Full=Uncharacterized protein ybbN; P77395
(YBBN_ECOLI)
2D-001WPC
4.45
34'596
{PMF}
-
1
yceI RecName: Full=Protein yceI; Flags: Precursor; P0A8X2
(YCEI_ECOLI)
2D-001WVT
5.17
21'096
{PMF,MS/MS}
-
1
ydbC RecName: Full=Putative oxidoreductase YdbC; EC=1.-.-.-; P25906
(YDBC_ECOLI)
2D-001WRR
5.32
29'821
{MS/MS}
-
1
yebR RecName: Full=Protein yebR; P76270
(YEBR_ECOLI)
2D-001WVX
4.88
20'670
{MS/MS}
Expression
1
ygaU RecName: Full=Uncharacterized protein ygaU; P0ADE6
(YGAU_ECOLI)
2D-001WXC
5.70
16'000
{MS/MS}
Expression
1
yhdH RecName: Full=Putative quinone oxidoreductase YhdH; EC=1.6.5.-; P26646
(YHDH_ECOLI)
2D-001WPY
5.58
33'803
{PMF}
-
1
yieL RecName: Full=Uncharacterized protein yieL; P31471
(YIEL_ECOLI)
2D-001WN7
5.78
39'021
{MS/MS}
-
1
yniC RecName: Full=Phosphatase YniC; EC=3.1.3.-; P77247
(YNIC_ECOLI)
2D-001WUA
4.84
24'436
{MS/MS}
-
1
zinT RecName: Full=Metal-binding protein ZinT; AltName: Full=Cadmium-induced protein ZinT; Flags: Precursor; P76344
(ZINT_ECOLI)
2D-001WU0
2D-001WU6
5.51
5.37
24'964
24'787
{MS/MS}
{MS/MS}
Expression
Expression
1
1
znuA RecName: Full=High-affinity zinc uptake system protein znuA; Flags: Precursor; P39172
(ZNUA_ECOLI)
2D-001WQW
2D-001WQO
5.49
5.47
32'476
32'751
{MS/MS}
{MS/MS}
Expression
Expression
1
1
Mapping legends: { MS/MS } => 'Tandem mass spectrometry', { PMF } => 'Peptide mass fingerprinting'



Cited references

# PubMed/Medline Authors Location
[1] 12469338 Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.; Proteomics 2(1):1682-1698(2002).
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'';


Gel experimental information

Information Type Data
Map name ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
Strain Comment [species] ER1647 [Escherichia coli / TaxID:562]
pI start: 4.50 - end: 6.50
Mw start: 10000 - end: 200000
Number of detected spots 858
Number of identified spots 174
Number of identified proteins 153
Reference Bibliographic reference
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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server