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SWISS-2DPAGE 
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Protein list for SWISS-2DPAGE ECOLI4-5 
Escherichia coli(4-5) 


(map ID: 22e0c978-9ced-4ecc-b207-32105b4fcfc6)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for ecoli4-5   70 identified spots / 33 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods References
accB RecName: Full=Biotin carboxyl carrier protein of acetyl-CoA carboxylase; Short=BCCP; P0ABD8
(BCCP_ECOLI)
2D-001HMN
2D-001HMO
2D-001HN0
4.74
5.01
4.78
18'383
18'505
15'857
{PMF}
{PMF}
{PMF}
1
1
1
adk RecName: Full=Adenylate kinase; Short=AK; EC=2.7.4.3; AltName: Full=ATP-AMP transphosphorylase; P69441
(KAD_ECOLI)
2D-001HQ8
5.00
9'979
{PMF}
1
atpD RecName: Full=ATP synthase subunit beta; EC=3.6.3.14; AltName: Full=ATP synthase F1 sector subunit beta; AltName: Full=F-ATPase subunit beta; P0ABB4
(ATPB_ECOLI)
2D-001HJ0
2D-001HJ1
2D-001HHU
4.95
4.55
4.95
43'643
43'745
47'782
{Gm, PMF}
{PMF}
{PMF}
1
1
1
cheY RecName: Full=Chemotaxis protein CheY; P0AE67
(CHEY_ECOLI)
2D-001HQO
4.95
9'749
{PMF}
1
crr RecName: Full=Glucose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIIA-Glc; AltName: Full=EIII-Glc; AltName: Full=PTS system glucose-specific EIIA component; P69783
(PTGA_ECOLI)
2D-001HNL
2D-001HN8
2D-001HN9
4.95
4.95
4.86
13'907
14'800
14'849
{PMF}
{Mi, PMF}
{PMF}
1
1
1
cysK RecName: Full=Cysteine synthase A; Short=CSase A; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase A; Short=OAS-TL A; AltName: Full=O-acetylserine sulfhydrylase A; AltName: Full=Sulfate starvation-induced protein 5; Short=SSI5; P0ABK5
(CYSK_ECOLI)
2D-001HM3
2D-001HLC
2D-001HLH
2D-001HLU
4.93
5.01
4.95
4.95
22'242
26'299
25'617
24'067
{PMF}
{PMF}
{PMF}
{Mi, PMF}
1
1
1
1
dnaK RecName: Full=Chaperone protein DnaK; AltName: Full=HSP70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=Heat shock protein 70; P0A6Y8
(DNAK_ECOLI)
2D-001HFW
2D-001HF6
2D-001HFC
2D-001HIJ
2D-001HFX
2D-001HFT
2D-001HH6
2D-001HFY
2D-001HFS
2D-001HFZ
2D-001HFV
4.95
4.98
4.98
5.00
4.96
4.97
4.93
4.95
4.96
4.48
4.95
60'559
70'671
67'488
44'877
60'841
61'125
52'435
60'700
61'267
59'582
60'700
{Gm, Mi, PMF}
{PMF}
{PMF}
{PMF}
{Gm}
{PMF}
{Gm, PMF}
{PMF}
{PMF}
{PMF}
{PMF}
1
1
1
1
1
1
1
1
1
1
1
elbB RecName: Full=Enhancing lycopene biosynthesis protein 2; AltName: Full=Sigma cross-reacting protein 27A; Short=SCRP-27A; P0ABU5
(ELBB_ECOLI)
2D-001HLF
4.92
25'786
{PMF}
1
eno RecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase; P0A6P9
(ENO_ECOLI)
2D-001HLY
4.74
22'910
{PMF}
1
flgL RecName: Full=Flagellar hook-associated protein 3; Short=HAP3; AltName: Full=Hook-filament junction protein; P29744
(FLGL_ECOLI)
2D-001HKJ
2D-001HKK
4.80
4.83
32'560
32'453
{PMF}
{PMF}
1
1
fliC RecName: Full=Flagellin; P04949
(FLIC_ECOLI)
2D-001HIA
2D-001HID
2D-001HIJ
2D-001HIB
2D-001HI9
2D-001HIC
2D-001HI8
4.98
4.55
5.00
4.61
4.63
4.54
4.58
45'932
45'507
44'877
45'507
45'613
45'507
45'401
{PMF}
{Mi}
{PMF}
{PMF}
{Mi, PMF}
{PMF}
{Mi, PMF}
1
1
1
1
1
1
1
ftsZ RecName: Full=Cell division protein ftsZ; P0A9A6
(FTSZ_ECOLI)
2D-001HJR
2D-001HJQ
4.77
4.83
39'129
39'220
{PMF}
{PMF}
1
1
galM RecName: Full=Aldose 1-epimerase; EC=5.1.3.3; AltName: Full=Galactose mutarotase; AltName: Full=Type-1 mutarotase; P0A9C3
(GALM_ECOLI)
2D-001HJQ
2D-001HJP
4.83
4.97
39'220
39'311
{PMF}
{PMF}
1
1
glnA RecName: Full=Glutamine synthetase; EC=6.3.1.2; AltName: Full=Glutamate--ammonia ligase; P0A9C5
(GLNA_ECOLI)
2D-001HLA
5.00
26'823
{PMF}
1
grcA RecName: Full=Autonomous glycyl radical cofactor; P68066
(GRCA_ECOLI)
2D-001HOT
2D-001HON
4.96
5.01
10'675
11'128
{PMF}
{PMF}
1
1
greA RecName: Full=Transcription elongation factor greA; AltName: Full=Transcript cleavage factor greA; P0A6W5
(GREA_ECOLI)
2D-001HNL
4.95
13'907
{PMF}
1
groL RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60; P0A6F5
(CH60_ECOLI)
2D-001HGR
2D-001HH2
2D-001HGT
2D-001HFT
2D-001HGK
2D-001HGU
4.93
4.84
4.95
4.97
4.99
4.95
55'185
52'802
55'185
61'125
56'877
55'185
{PMF}
{PMF}
{PMF}
{PMF}
{Gm, PMF}
{Gm, PMF}
1
1
1
1
1
1
hdeA RecName: Full=Chaperone-like protein hdeA; AltName: Full=10K-S protein; Flags: Precursor; P0AES9
(HDEA_ECOLI)
2D-001HR2
4.84
9'475
{Mi,PMF}
1
hisJ RecName: Full=Histidine-binding periplasmic protein; Short=HBP; Flags: Precursor; P0AEU0
(HISJ_ECOLI)
2D-001HPA
4.99
10'511
{PMF}
1
hldD RecName: Full=ADP-L-glycero-D-manno-heptose-6-epimerase; EC=5.1.3.20; AltName: Full=ADP-L-glycero-beta-D-manno-heptose-6-epimerase; Short=ADP-glyceromanno-heptose 6-epimerase; Short=ADP-hep 6-epimerase; Short=AGME; P67910
(HLDD_ECOLI)
2D-001HK8
4.98
35'245
{PMF}
1
icd RecName: Full=Isocitrate dehydrogenase [NADP]; Short=IDH; EC=1.1.1.42; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase; P08200
(IDH_ECOLI)
2D-001HM1
4.70
22'389
{PMF}
1
metQ RecName: Full=D-methionine-binding lipoprotein metQ; Flags: Precursor; P28635
(METQ_ECOLI)
2D-001HOI
2D-001HOB
4.95
4.95
11'539
11'934
{PMF}
{PMF}
1
1
mog RecName: Full=Molybdopterin adenylyltransferase; Short=MPT adenylyltransferase; EC=2.7.7.75; P0AF03
(MOG_ECOLI)
2D-001HQI
4.84
9'800
{PMF}
1
nfuA RecName: Full=Fe/S biogenesis protein nfuA; P63020
(NFUA_ECOLI)
2D-001HMI
2D-001HMJ
4.72
4.96
19'761
19'503
{PMF}
{PMF}
1
1
potD RecName: Full=Spermidine/putrescine-binding periplasmic protein; Short=SPBP; Flags: Precursor; P0AFK9
(POTD_ECOLI)
2D-001HKA
2D-001HK9
4.76
4.95
34'885
35'000
{PMF}
{Gm, PMF}
1
1
rplL RecName: Full=50S ribosomal protein L7/L12; AltName: Full=L8; P0A7K2
(RL7_ECOLI)
2D-001HQK
2D-001HQJ
2D-001HQL
4.71
4.87
4.74
9'775
9'851
9'851
{PMF}
{PMF}
{PMF}
1
1
1
rpsA RecName: Full=30S ribosomal protein S1; P0AG67
(RS1_ECOLI)
2D-001HFK
4.99
64'780
{Gm}
1
sspB RecName: Full=Stringent starvation protein B; P0AFZ3
(SSPB_ECOLI)
2D-001HMG
5.01
20'288
{PMF}
1
tig RecName: Full=Trigger factor; Short=TF; P0A850
(TIG_ECOLI)
2D-001HHV
2D-001HIE
2D-001HHI
2D-001HHJ
4.97
4.87
4.96
4.58
47'893
45'401
49'707
49'476
{PMF}
{PMF}
{Gm, PMF}
{PMF}
1
1
1
1
tpiA RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase; P0A858
(TPIS_ECOLI)
2D-001HON
5.01
11'128
{PMF}
1
tpx RecName: Full=Thiol peroxidase; EC=1.11.1.-; AltName: Full=Scavengase P20; P0A862
(TPX_ECOLI)
2D-001HN3
5.02
15'095
{Gm}
1
trxA RecName: Full=Thioredoxin-1; Short=Trx-1; P0AA25
(THIO_ECOLI)
2D-001HQU
4.80
9'699
{PMF}
1
yjdC RecName: Full=HTH-type transcriptional regulator yjdC; P0ACU7
(YJDC_ECOLI)
2D-001HMA
5.00
20'965
{PMF}
1
Mapping legends: { Gm } => 'Gel matching', { Mi } => 'Microsequencing', { PMF } => 'Peptide mass fingerprinting'



Cited references

# PubMed/Medline Authors Location
[1] 11680886 Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.; Proteomics 1(1):409-423(2001).
''''''New perspectives in the Escherichia coli proteome investigation'';'';'';


Gel experimental information

Information Type Data
Map name ECOLI4-5 {Escherichia coli(4-5)}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
Strain Comment [species] W3110 [Escherichia coli / TaxID:562]
pI start: 4.00 - end: 5.00
Mw start: 10000 - end: 200000
Number of detected spots 449
Number of identified spots 70
Number of identified proteins 33
Reference Bibliographic reference
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SWISS-2DPAGE image

SWISS-2DPAGE (search Map)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server