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SWISS-2DPAGE 
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Protein list for SWISS-2DPAGE YEAST 
Saccharomyces cerevisiae  {Saccharomyces cerevisiae (Baker's yeast)}


(map ID: 9afea53f-7466-4871-8e84-60e0cc02446d)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for yeast   101 identified spots / 63 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods References
- RecName: Full=Unknown protein from spot 2D-000JYC of 2D-PAGE; Flags: Fragment; P99012
(UXB1_YEASX)
2D-000JYC
5.72
12'401
{Mi}
1
ACT1 RecName: Full=Actin; P60010
(ACT_YEAST)
2D-000J9F
5.40
42'600
{Gm}
1, 2
ADE1 RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase; EC=6.3.2.6; AltName: Full=SAICAR synthetase; P27616
(PUR7_YEAST)
2D-000JI2
5.64
32'116
{Gm}
1, 3
ADH1 RecName: Full=Alcohol dehydrogenase 1; EC=1.1.1.1; AltName: Full=Alcohol dehydrogenase I; AltName: Full=YADH-1; P00330
(ADH1_YEAST)
2D-000JAP
2D-000JAV
2D-000JAF
6.45
6.69
6.60
41'184
40'924
41'097
{Gm,Aa}
{Gm,Aa}
{Gm,Aa}
1, 2
1, 2
1, 2
ADK1 RecName: Full=Adenylate kinase 1; Short=AK 1; EC=2.7.4.3; AltName: Full=ATP-AMP transphosphorylase 1; AltName: Full=Adenylate kinase cytosolic and mitochondrial; Flags: Precursor; P07170
(KAD1_YEAST)
2D-000JRN
5.92
23'293
{Gm}
1, 3
ATP2 RecName: Full=ATP synthase subunit beta, mitochondrial; EC=3.6.3.14; Flags: Precursor; P00830
(ATPB_YEAST)
2D-000J5L
5.06
48'143
{Gm}
1, 3
BMH1 RecName: Full=Protein BMH1; P29311
(BMH1_YEAST)
2D-000JJ8
4.68
30'842
{Gm}
1, 4
BMH2 RecName: Full=Protein BMH2; P34730
(BMH2_YEAST)
2D-000JHY
4.69
32'361
{Gm}
1, 4
CPR5 RecName: Full=Peptidyl-prolyl cis-trans isomerase D; Short=PPIase D; EC=5.2.1.8; AltName: Full=Cyclophilin D; AltName: Full=Rotamase D; Flags: Precursor; P35176
(CYPD_YEAST)
2D-000JTB
5.33
21'873
{Gm}
1, 3
EFB1 RecName: Full=Elongation factor 1-beta; Short=EF-1-beta; AltName: Full=Eukaryotic elongation factor 1Balpha; Short=eEF1Balpha; AltName: Full=Translation elongation factor 1B alpha; P32471
(EF1B_YEAST)
2D-000JJZ
4.34
29'769
{Aa}
1, 3
EFT1 RecName: Full=Elongation factor 2; Short=EF-2; AltName: Full=Eukaryotic elongation factor 2; Short=eEF2; AltName: Full=Ribosomal translocase; AltName: Full=Translation elongation factor 2; P32324
(EF2_YEAST)
2D-000IPZ
2D-000IQ8
2D-000IQ7
6.20
6.26
5.92
98'328
98'052
98'052
{Gm}
{Gm}
{Gm}
1, 3
1, 3
1, 3
ENO1 RecName: Full=Enolase 1; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase 1; AltName: Full=2-phosphoglycerate dehydratase 1; P00924
(ENO1_YEAST)
2D-000J69
2D-000J6J
6.50
6.61
46'545
46'284
{Mi,Gm,Aa}
{Mi,Gm,Aa}
1, 2, 5
1, 2, 5
ENO2 RecName: Full=Enolase 2; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase 2; AltName: Full=2-phosphoglycerate dehydratase 2; P00925
(ENO2_YEAST)
2D-000J73
2D-000JZS
2D-000J7C
6.00
6.15
6.17
45'000
11'465
45'127
{Mi,Gm,Aa}
{Mi,Gm,Aa}
{Mi,Gm,Aa}
1, 2, 5
1, 2, 5
1, 2, 5
ENO2 RecName: Full=Enolase 2; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase 2; AltName: Full=2-phosphoglycerate dehydratase 2; P99013
(UXB2_YEAST)
2D-000K2F
6.19
9'191
{Mi}
1
ENO2 RecName: Full=Enolase 2; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase 2; AltName: Full=2-phosphoglycerate dehydratase 2; P00925
(ENO2_YEAST)
2D-000J7A
2D-000J87
5.87
5.76
45'127
44'222
{Mi,Gm,Aa}
{Mi,Gm,Aa}
1, 2, 5
1, 2, 5
ERG6 RecName: Full=Sterol 24-C-methyltransferase; EC=2.1.1.41; AltName: Full=Delta(24)-sterol C-methyltransferase; P25087
(ERG6_YEAST)
2D-000J9O
5.53
42'510
{Gm}
1, 3
FBA1 RecName: Full=Fructose-bisphosphate aldolase; Short=FBP aldolase; Short=FBPA; EC=4.1.2.13; AltName: Full=Fructose-1,6-bisphosphate aldolase; P14540
(ALF_YEAST)
2D-000JEW
2D-000JER
2D-000JEQ
5.75
5.99
5.87
36'899
37'212
36'899
{Mi,Aa}
{Mi,Aa}
{Mi,Aa}
1, 3
1, 3
1, 3
GLN1 RecName: Full=Glutamine synthetase; Short=GS; EC=6.3.1.2; AltName: Full=Glutamate--ammonia ligase; P32288
(GLNA_YEAST)
2D-000JAE
6.08
41'709
{Gm}
1, 6
GPM1 RecName: Full=Phosphoglycerate mutase 1; Short=PGAM 1; EC=5.4.2.1; AltName: Full=BPG-dependent PGAM 1; AltName: Full=MPGM 1; AltName: Full=Phosphoglyceromutase 1; P00950
(PMG1_YEAST)
2D-000JPA
2D-000JPN
2D-000JP4
2D-000JPJ
2D-000JQ8
2D-000JP8
9.14
8.73
8.68
8.08
9.54
7.86
25'384
25'384
25'969
25'320
24'876
25'707
{Mi}
{Mi}
{Mi}
{Mi}
{Mi}
{Mi}
1
1
1
1
1
1
GSP1 RecName: Full=GTP-binding nuclear protein GSP1/CNR1; AltName: Full=Chromosome stability protein 17; AltName: Full=GTPase Ran homolog; AltName: Full=Genetic suppressor of PRP20-1; P32835
(GSP1_YEAST)
2D-000JQH
6.08
24'439
{Gm}
1, 3
GYP6 RecName: Full=GTPase-activating protein GYP6; AltName: Full=GAP for YPT6; P32806
(GYP6_YEAST)
2D-000IYU
6.05
59'283
{Gm}
1, 3
HOM2 RecName: Full=Aspartate-semialdehyde dehydrogenase; Short=ASA dehydrogenase; Short=ASADH; EC=1.2.1.11; AltName: Full=Aspartate-beta-semialdehyde dehydrogenase; P13663
(DHAS_YEAST)
2D-000JCE
6.66
39'396
{Gm}
1, 3
HSC82 RecName: Full=ATP-dependent molecular chaperone HSC82; AltName: Full=82 kDa heat shock cognate protein; AltName: Full=Heat shock protein Hsp90 constitutive isoform; P15108
(HSC82_YEAST)
2D-000IRA
4.78
88'619
{Gm}
1, 7
HSP104 RecName: Full=Heat shock protein 104; AltName: Full=Protein aggregation-remodeling factor HSP104; P31539
(HS104_YEAST)
2D-000IPL
2D-000IPK
2D-000IPM
5.33
5.28
5.36
100'000
101'097
100'000
{Gm}
{Gm}
{Gm}
1, 8
1, 8
1, 8
HSP60 RecName: Full=Heat shock protein 60, mitochondrial; AltName: Full=CPN60; AltName: Full=P66; AltName: Full=Stimulator factor I 66 kDa component; Flags: Precursor; P19882
(HSP60_YEAST)
2D-000IX3
2D-000IWY
4.92
4.89
65'049
65'232
{Mi}
{Mi}
1, 3
1, 3
HSP82 RecName: Full=ATP-dependent molecular chaperone HSP82; AltName: Full=82 kDa heat shock protein; AltName: Full=Heat shock protein Hsp90 heat-inducible isoform; P02829
(HSP82_YEAST)
2D-000IRA
4.78
88'619
{Gm}
1, 3
HXK1 RecName: Full=Hexokinase-1; EC=2.7.1.1; AltName: Full=Hexokinase PI; AltName: Full=Hexokinase-A; P04806
(HXKA_YEAST)
2D-000J0U
5.33
55'102
{Gm}
1, 3
HXK2 RecName: Full=Hexokinase-2; EC=2.7.1.1; AltName: Full=Hexokinase PII; AltName: Full=Hexokinase-B; P04807
(HXKB_YEAST)
2D-000J1A
2D-000J12
5.27
5.17
54'793
55'102
{Gm}
{Gm}
1, 3
1, 3
HYP2 RecName: Full=Eukaryotic translation initiation factor 5A-1; Short=eIF-5A-1; AltName: Full=Hypusine-containing protein HP2; AltName: Full=eIF-4D; P23301
(IF5A1_YEAST)
2D-000JWT
2D-000JWS
4.66
4.76
15'434
15'388
{Aa}
{Aa}
1, 9
1, 9
ILV5 RecName: Full=Ketol-acid reductoisomerase, mitochondrial; EC=1.1.1.86; AltName: Full=Acetohydroxy-acid reductoisomerase; AltName: Full=Alpha-keto-beta-hydroxylacil reductoisomerase; Flags: Precursor; P06168
(ILV5_YEAST)
2D-000JC8
6.70
39'563
{Gm}
1, 3
IPP1 RecName: Full=Inorganic pyrophosphatase; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase; Short=PPase; P00817
(IPYR_YEAST)
2D-000JI1
5.41
32'035
{Aa}
1, 2
KAR2 RecName: Full=78 kDa glucose-regulated protein homolog; Short=GRP-78; AltName: Full=Immunoglobulin heavy chain-binding protein homolog; Short=BiP; Flags: Precursor; P16474
(GRP78_YEAST)
2D-000ITJ
4.76
78'975
{Gm}
1, 7
LEU2 RecName: Full=3-isopropylmalate dehydrogenase; Short=3-IPM-DH; Short=IMDH; EC=1.1.1.85; AltName: Full=Beta-IPM dehydrogenase; P04173
(LEU3_YEAST)
2D-000JB2
5.48
40'837
{Gm}
1, 3
MET6 RecName: Full=5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; EC=2.1.1.14; AltName: Full=Cobalamin-independent methionine synthase; AltName: Full=Delta-P8 protein; AltName: Full=Methionine synthase, vitamin-B12 independent isozyme; P05694
(METE_YEAST)
2D-000IRM
2D-000IRS
2D-000IRQ
6.27
6.47
6.36
86'893
86'163
86'406
{Gm}
{Gm}
{Gm}
1, 3
1, 3
1, 3
MMF1 RecName: Full=Protein MMF1, mitochondrial; AltName: Full=Isoleucine biosynthesis and maintenance of intact mitochondria 1; AltName: Full=Maintenance of mitochondrial function 1; Flags: Precursor; P40185
(MMF1_YEAST)
2D-000K01
6.89
11'183
{Mi}
1
PDB1 RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; EC=1.2.4.1; AltName: Full=Pyruvate dehydrogenase complex component E1 beta; Short=PDHE1-B; Flags: Precursor; P32473
(ODPB_YEAST)
2D-000JI6
4.84
32'116
{Gm}
1, 3
PDC1 RecName: Full=Pyruvate decarboxylase isozyme 1; EC=4.1.1.-; EC=4.1.1.1; P06169
(PDC1_YEAST)
2D-000IYT
2D-000IZA
2D-000IZ4
5.90
5.65
5.77
58'619
58'784
58'619
{Gm}
{Gm}
{Gm}
1, 5
1, 5
1, 5
REI1 RecName: Full=Pre-60S factor REI1; AltName: Full=Required for isotropic bud growth protein 1; P38344
(REI1_YEAST)
2D-000JF1
4.85
37'055
{Gm}
1, 3
RPP0 RecName: Full=60S acidic ribosomal protein P0; Short=A0; AltName: Full=L10E; P05317
(RLA0_YEAST)
2D-000JHR
4.78
32'525
{Gm}
1, 3
RPS0A RecName: Full=40S ribosomal protein S0-A; AltName: Full=Nucleic acid-binding protein NAB1A; P32905
(RSSA1_YEAST)
2D-000JJS
2D-000JJR
4.72
4.68
30'148
30'301
{Mi}
{Mi}
1, 3
1, 3
RPT1 RecName: Full=26S protease regulatory subunit 7 homolog; AltName: Full=Protein CIM5; AltName: Full=Tat-binding homolog 3; P33299
(PRS7_YEAST)
2D-000IY6
5.21
60'632
{Gm}
1, 3
SBA1 RecName: Full=Co-chaperone protein SBA1; P28707
(SBA1_YEAST)
2D-000JMV
4.60
27'247
{Gm}
1, 3
SEC53 RecName: Full=Phosphomannomutase; Short=PMM; EC=5.4.2.8; P07283
(PMM_YEAST)
2D-000JQD
4.92
24'563
{Gm}
1, 3
SER1 RecName: Full=Phosphoserine aminotransferase; Short=PSAT; EC=2.6.1.52; AltName: Full=Phosphohydroxythreonine aminotransferase; P33330
(SERC_YEAST)
2D-000J8X
6.37
43'242
{Gm}
1, 3
SOD1 RecName: Full=Superoxide dismutase [Cu-Zn]; EC=1.15.1.1; P00445
(SODC_YEAST)
2D-000JXB
5.82
14'208
{Mi}
1, 3
SSA1 RecName: Full=Heat shock protein SSA1; AltName: Full=Heat shock protein YG100; P10591
(HSP71_YEAST)
2D-000IUM
4.89
73'205
{Gm}
1, 7
SSA2 RecName: Full=Heat shock protein SSA2; P10592
(HSP72_YEAST)
2D-000IUH
4.82
73'825
{Gm}
1, 7
SSB1 RecName: Full=Heat shock protein SSB1; AltName: Full=Cold-inducible protein YG101; P11484
(HSP75_YEAST)
2D-000IVS
2D-000IVT
2D-000IVO
5.23
5.29
5.16
69'396
69'396
69'396
{Gm}
{Gm}
{Gm}
1, 7
1, 7
1, 7
SSC1 RecName: Full=Heat shock protein SSC1, mitochondrial; AltName: Full=Endonuclease SceI 75 kDa subunit; Short=Endo.SceI 75 kDa subunit; AltName: Full=mtHSP70; Flags: Precursor; P12398
(HSP77_YEAST)
2D-000IUI
2D-000IUE
5.17
5.12
74'033
74'241
{Gm}
{Gm}
1, 7
1, 7
SSE1 RecName: Full=Heat shock protein homolog SSE1; AltName: Full=Chaperone protein MSI3; P32589
(HSP7F_YEAST)
2D-000IT1
2D-000IT0
5.14
5.11
82'840
82'840
{Gm}
{Gm}
1, 3
1, 3
STI1 RecName: Full=Heat shock protein STI1; P15705
(STI1_YEAST)
2D-000ITV
5.48
75'929
{Gm}
1, 7
TAL1 RecName: Full=Transaldolase; EC=2.2.1.2; P15019
(TAL1_YEAST)
2D-000JGF
6.00
35'000
{Gm}
1, 3
TDH1 RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 1; Short=GAPDH 1; EC=1.2.1.12; P00360
(G3P1_YEAST)
2D-000K0I
6.98
10'985
{Mi}
1
TDH2 RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 2; Short=GAPDH 2; EC=1.2.1.12; P00358
(G3P2_YEAST)
2D-000JIM
2D-000JIK
2D-000JIL
2D-000JU5
2D-000JYO
2D-000JIE
6.77
6.72
6.81
6.59
6.73
6.65
31'793
31'954
31'954
20'618
12'009
32'035
{Mi,Gm,Aa}
{Mi,Gm,Aa}
{Mi,Gm,Aa}
{Mi,Gm,Aa}
{Mi,Gm,Aa}
{Mi,Gm,Aa}
1, 10
1, 10
1, 10
1, 10
1, 10
1, 10
TDH3 RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 3; Short=GAPDH 3; EC=1.2.1.12; P00359
(G3P3_YEAST)
2D-000JH9
2D-000JHL
2D-000JHS
6.81
6.73
6.88
32'772
32'772
32'607
{Mi,Gm,Aa}
{Mi,Gm,Aa}
{Mi,Gm,Aa}
1, 10
1, 10
1, 10
TIF1 RecName: Full=ATP-dependent RNA helicase eIF4A; EC=3.6.4.13; AltName: Full=Eukaryotic initiation factor 4A; Short=eIF-4A; AltName: Full=Stimulator factor I 37 kDa component; AltName: Full=Translation initiation factor 1/2; AltName: Full=p37; P10081
(IF4A_YEAST)
2D-000J7E
4.95
45'000
{Aa}
1, 3
TKL1 RecName: Full=Transketolase 1; Short=TK 1; EC=2.2.1.1; P23254
(TKT1_YEAST)
2D-000IUR
2D-000IUQ
2D-000IUT
6.70
6.64
6.76
73'000
73'000
72'795
{Gm}
{Gm}
{Gm}
1, 3
1, 3
1, 3
TMA19 RecName: Full=Translationally-controlled tumor protein homolog; Short=TCTP; AltName: Full=Microtubule and mitochondria-interacting protein 1; AltName: Full=Translation machinery-associated protein 19; P35691
(TCTP_YEAST)
2D-000JS9
4.50
23'000
{Mi}
1
TPI1 RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase; P00942
(TPIS_YEAST)
2D-000JRP
2D-000JS0
6.01
5.64
23'175
23'058
{Mi,Gm,Aa}
{Mi,Gm,Aa}
1, 2
1, 2
TSA1 RecName: Full=Peroxiredoxin TSA1; EC=1.11.1.15; AltName: Full=Cytoplasmic thiol peroxidase 1; Short=cTPx 1; AltName: Full=PRP; AltName: Full=Thiol-specific antioxidant protein 1; AltName: Full=Thioredoxin peroxidase; P34760
(TSA1_YEAST)
2D-000JUJ
4.97
20'136
{Gm}
1, 3
UBI4 RecName: Full=Polyubiquitin; Contains: RecName: Full=Ubiquitin; Flags: Precursor; P0CG63
(UBI4P_YEAST)
2D-000K2B
6.79
9'323
{Mi}
1
VMA4 RecName: Full=V-type proton ATPase subunit E; Short=V-ATPase subunit E; AltName: Full=V-ATPase 27 kDa subunit; AltName: Full=Vacuolar proton pump subunit E; P22203
(VATE_YEAST)
2D-000JQN
5.17
24'316
{Gm}
1, 3
ZWF1 RecName: Full=Glucose-6-phosphate 1-dehydrogenase; Short=G6PD; EC=1.1.1.49; P11412
(G6PD_YEAST)
2D-000IZJ
6.08
57'638
{Gm}
1, 3
Mapping legends: { Aa } => 'Amino acid composition', { Gm } => 'Gel matching', { Mi } => 'Microsequencing'



Cited references

# PubMed/Medline Authors Location
[1] 8740180 Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.; Electrophoresis 17(1):556-565(1996).
''''''The yeast SWISS-2DPAGE database'';'';'';
[2] 7050667 Ludwig J.R., Foy J.J., Elliott S.G., McLaughlin C.S.; Mol. Cell. Biol. 2(1):117-126(1982).
''''''Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae'';'';'';
[3] 7895733 Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.; Electrophoresis 15(1):1466-1486(1994).
''''''Protein identifications for a Saccharomyces cerevisiae protein database'';'';'';
[4] 1286666 Celis J.E., Rasmussen H.H., Madsen P., Leffers H., Honore B., Dejgaard K., Gesser B., Olsen E., Gromov P., Hoffmann H.J., Nielsen M., Celis A., Basse B., Lauridsen J.B., Ratz G.P., Nielsen H., Walbum E., Kjaegaard I., Puype M., Van Damme J., Vandekerckhove J.; Electrophoresis 13(1):893-959(1992).
''''''The human keratinocyte two-dimensional gel protein database (update 1992): towards an integrated approach to the study of cell proliferation, differentiation and skin diseases'';'';'';
[5] 3332961 Bataille N., Peypouquet M.-F., Boucherie H.; Yeast 3(1):11-21(1987).
''''''Identification of polypeptides of the carbon metabolism machinery on the two-dimensional protein map of Saccharomyces cerevisiae. Location of 23 additional polypeptides'';'';'';
[6] 6129248 Mitchell A., Magasanik B.; J. Biol. Chem. 258(1):610-615(1983).
''''''Purification and properties of glutamine synthetase from Saccharomyces cerevisiae'';'';'';
[7] 2005818 Nicolet C.M., Craig E.A.; Meth. Enzymol. 194(1):710-717(1991).
''''''Inducing and assaying heat-shock response in Saccharomyces cerevisiae'';'';'';
[8] 8407824 Sanchez Y., Parsell D.A., Taulien J., Vogel J.L., Craig E.A., Lindquist S.; J. Bacteriol. 175(1):6484-6491(1993).
''''''Genetic evidence for a functional relationship between Hsp104 and Hsp70'';'';'';
[9] 1281091 Klier H., Lottspeich F.; Electrophoresis 13(1):732-735(1992).
''''''Detection of the hypusine-containing protein (HP = eIF-5A) in crude yeast extracts by two-dimensional western blots'';'';'';
[10] 2999100 McAlister L., Holland M.J.; J. Biol. Chem. 260(1):15019-15027(1985).
''''''Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes'';'';'';


Gel experimental information

Information Type Data
Map name YEAST {Saccharomyces cerevisiae}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
Strain Comment [species] X2180-1A [Saccharomyces cerevisiae (Baker's yeast) / TaxID:4932]
pI start: 3.50 - end: 10.00
Mw start: 10000 - end: 200000
Number of detected spots 1940
Number of identified spots 101
Number of identified proteins 62
Reference Bibliographic reference
Graphical interface
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SWISS-2DPAGE image

SWISS-2DPAGE (search Map)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server