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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: P00963



General information about the entry
View entry in simple text format
Entry nameASNA_ECOLI
Primary accession numberP00963
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=Aspartate--ammonia ligase; EC=; AltName: Full=Asparagine synthetase A;.
Gene nameName=asnA
OrderedLocusNames=b3744, JW3722
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
  • SUBUNIT: Homodimer
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
  map experimental info
  protein estimated location

pI=5.45; Mw=37876  [identification data]
pI=5.38; Mw=37715  [identification data]

decrease after benzoic acid treatment [1].

MAPPING (identification):
Tandem mass spectrometry [1].

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
UniProtKB/Swiss-ProtP00963; ASNA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameASNA_ECOLI
Primary accession numberP00963
Secondary accession number(s) Q2M862
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 108)
Name and origin of the protein
DescriptionRecName: Full=Aspartate--ammonia ligase; EC=; AltName: Full=Asparagine synthetase A;
Gene nameName=asnA
OrderedLocusNames=b3744, JW3722
Encoded onName=asnA; OrderedLocusNames=b3744, JW3722
Keywords3D-structure; Amino-acid biosynthesis; Asparagine biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Reference proteome.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLV00263; CAA23512.1; ALT_SEQ; Genomic_DNA
EMBLK00826; AAA24253.1; -; Genomic_DNA
EMBLJ01657; AAA24248.1; -; Genomic_DNA
EMBLL10328; AAA62096.1; -; Genomic_DNA
EMBLU00096; AAC76767.1; -; Genomic_DNA
EMBLAP009048; BAE77544.1; -; Genomic_DNA
EMBLM10679; AAA24249.1; -; Genomic_DNA
PIRA01191; AJECNA; .
RefSeqNP_418200.1; NC_000913.2; .
PDB11AS; X-ray; 2.50 A; A/B=1-330
PDB12AS; X-ray; 2.20 A; A/B=1-330
PDBsum11AS; -; .
PDBsum12AS; -; .
ProteinModelPortalP00963; -; .
SMRP00963; 4-330; .
DIPDIP-9176N; -; .
IntActP00963; 4; .
SWISS-2DPAGEP00963; -; .
EnsemblBacteriaEBESCT00000001244; EBESCP00000001244; EBESCG00000001030; .
EnsemblBacteriaEBESCT00000017488; EBESCP00000016779; EBESCG00000016544; .
GeneID948258; -; .
GenomeReviewsAP009048_GR; JW3722; .
GenomeReviewsU00096_GR; b3744; .
KEGGecj:JW3722; -; .
KEGGeco:b3744; -; .
EchoBASEEB0089; -; .
EcoGeneEG10091; asnA; .
eggNOGCOG2502; -; .
GeneTreeEBGT00050000010820; -; .
HOGENOMHBG288146; -; .
ProtClustDBPRK05425; -; .
DrugBankDB00131; Adenosine monophosphate; .
GenevestigatorP00963; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0004812; F:aminoacyl-tRNA ligase activity; IEA:InterPro; .
GOGO:0004071; F:aspartate-ammonia ligase activity; IEA:EC; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0006529; P:asparagine biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro; .
HAMAPMF_00555; AsnA; 1; -
InterProIPR006195; aa-tRNA-synth_II; .
InterProIPR004618; AsnA; .
PfamPF03590; AsnA; 1; .
PIRSFPIRSF001555; Asp_ammon_ligase; 1; .
TIGRFAMsTIGR00669; AsnA; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server