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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P01011




SWISS-2DPAGE:  P01011


P01011


General information about the entry
View entry in simple text format
Entry nameAACT_HUMAN
Primary accession numberP01011
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Alpha-1-antichymotrypsin; Short=ACT; AltName: Full=Cell growth-inhibiting gene 24/25 protein; AltName: Full=Serpin A3; Contains: RecName: Full=Alpha-1-antichymotrypsin His-Pro-less; Flags: Precursor;.
Gene nameName=SERPINA3
Synonyms=AACT
ORFNames=GIG24, GIG25
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=78094420; PubMed=271964; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Anderson N.G.
''''''High resolution two-dimensional electrophoresis of human plasma proteins'';'';''
Proc. Natl. Acad. Sci. U.S.A. 74(1):5421-5425(1977)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
Comments
  • SUBUNIT: SINGLE CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=4.40; Mw=70976
pI=4.44; Mw=70204
pI=4.48; Mw=67939
pI=4.54; Mw=66132
pI=4.58; Mw=65462
pI=6.99; Mw=64964
pI=4.62; Mw=64469
pI=4.65; Mw=63492

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=4.45; Mw=66345
pI=4.37; Mw=67000
pI=4.41; Mw=66781
pI=4.49; Mw=65912
pI=4.55; Mw=65482
pI=4.61; Mw=65268
pI=4.65; Mw=64630

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=4.58; Mw=65028
pI=4.61; Mw=64501
pI=4.57; Mw=65205
pI=4.64; Mw=63978
pI=4.67; Mw=63459
pI=4.70; Mw=62603
pI=4.73; Mw=61591
pI=4.59; Mw=60926
pI=4.67; Mw=61591
pI=4.76; Mw=60596
pI=4.63; Mw=60596
pI=4.70; Mw=60596
pI=4.67; Mw=59941
pI=4.79; Mw=59941
pI=4.72; Mw=59294
pI=4.67; Mw=58973
pI=4.70; Mw=58973
pI=4.76; Mw=58178
pI=4.72; Mw=57862
pI=4.76; Mw=56773

NORMAL LEVEL:
300-600 MG/L.

MAPPING (identification):
IMMUNOBLOTTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
DOSAC-COBS 2D-PAGEP01011; P01011.
DOSAC-COBS-2DPAGEP01011; AACT_HUMAN.
Siena-2DPAGEP01011; AACT_HUMAN.
UniProtKB/Swiss-ProtP01011; AACT_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameAACT_HUMAN
Primary accession numberP01011
Secondary accession number(s) B3KVQ7 Q13703 Q2TU87 Q2TU88 Q59GP9 Q6LBY8 Q6LDT7 Q6NSC9 Q8N177 Q96DW8 Q9UC47 Q9UNU9
Sequence was last modified on August 1, 1991 (version 2)
Annotations were last modified on September 21, 2011 (version 152)
Name and origin of the protein
DescriptionRecName: Full=Alpha-1-antichymotrypsin; Short=ACT; AltName: Full=Cell growth-inhibiting gene 24/25 protein; AltName: Full=Serpin A3; Contains: RecName: Full=Alpha-1-antichymotrypsin His-Pro-less; Flags: Precursor;
Gene nameName=SERPINA3
Synonyms=AACT
ORFNames=GIG24, GIG25
Encoded onName=SERPINA3; Synonyms=AACT; ORFNames=GIG24, GIG25
Keywords3D-structure; Acute phase; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Glycoprotein; Polymorphism; Protease inhibitor; Reference proteome; Secreted; Serine protease inhibitor; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK01500; AAA51543.1; ALT_FRAME; mRNA
EMBLX68733; CAA48671.1; ALT_INIT; Genomic_DNA
EMBLX68734; CAA48671.1; JOINED; Genomic_DNA
EMBLX68735; CAA48671.1; JOINED; Genomic_DNA
EMBLX68736; CAA48671.1; JOINED; Genomic_DNA
EMBLX68737; CAA48671.1; JOINED; Genomic_DNA
EMBLAY513275; AAT08028.1; -; mRNA
EMBLAY513276; AAT08029.1; ALT_SEQ; mRNA
EMBLAK123091; BAG53869.1; -; mRNA
EMBLAB209060; BAD92297.1; ALT_INIT; mRNA
EMBLBC003559; AAH03559.3; -; mRNA
EMBLBC010530; AAH10530.1; -; mRNA
EMBLBC013189; AAH13189.1; -; mRNA
EMBLBC034554; AAH34554.1; -; mRNA
EMBLBC070265; AAH70265.1; -; mRNA
EMBLM18906; AAA51559.1; -; mRNA
EMBLAF089747; AAD08810.1; -; mRNA
EMBLJ05176; AAA51560.1; -; mRNA
EMBLX00947; CAA25459.1; -; Genomic_DNA
IPIIPI00550991; -; .
IPIIPI00607870; -; .
IPIIPI00847635; -; .
PIRA90475; ITHUC; .
PIRS62374; S62374; .
RefSeqNP_001076.2; NM_001085.4; .
UniGeneHs.534293; -; .
UniGeneHs.710488; -; .
PDB1AS4; X-ray; 2.10 A; A=43-383
B=387-423PDB; 1QMN; X-ray; 2.27 A
A=26-423PDB; 2ACH; X-ray; 2.70 A
A=24-383B=384-423; PDB; 3CAA; X-ray
2.40 AA=43-383; B=387-423; PDB; 3DLW
X-ray2.70 A; A=25-423; PDB; 4CAA
X-ray2.90 A; A=43-383; B=387-423; PDBsum
1AS4-; .
PDBsum1QMN; -; .
PDBsum2ACH; -; .
PDBsum3CAA; -; .
PDBsum3DLW; -; .
PDBsum4CAA; -; .
ProteinModelPortalP01011; -; .
SMRP01011; 48-422; .
IntActP01011; 1; .
STRINGP01011; -; .
MEROPSI04.002; -; .
GlycoSuiteDBP01011; -; .
PhosphoSiteP01011; -; .
SWISS-2DPAGEP01011; -; .
DOSAC-COBS-2DPAGEP01011; -; .
Siena-2DPAGEP01011; -; .
PRIDEP01011; -; .
EnsemblENST00000393078; ENSP00000376793; ENSG00000196136; .
EnsemblENST00000393080; ENSP00000376795; ENSG00000196136; .
GeneID12; -; .
KEGGhsa:12; -; .
UCSCuc001ydo.2; human; .
CTD12; -; .
GeneCardsGC14P075259; -; .
H-InvDBHIX0011931; -; .
HGNCHGNC:16; SERPINA3; .
HPACAB016647; -; .
HPAHPA000893; -; .
HPAHPA002560; -; .
MIM107280; gene; .
neXtProtNX_P01011; -; .
PharmGKBPA35020; -; .
eggNOGprNOG14001; -; .
GeneTreeENSGT00560000076583; -; .
HOVERGENHBG005957; -; .
InParanoidP01011; -; .
OrthoDBEOG4FFD1T; -; .
NextBio23; -; .
PMAP-CutDBP01011; -; .
ArrayExpressP01011; -; .
BgeeP01011; -; .
GenevestigatorP01011; -; .
GermOnlineENSG00000196136; Homo sapiens; .
GOGO:0005576; C:extracellular region; NAS:UniProtKB; .
GOGO:0005634; C:nucleus; TAS:UniProtKB; .
GOGO:0003677; F:DNA binding; TAS:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:UniProtKB; .
GOGO:0004867; F:serine-type endopeptidase inhibitor activity; TAS:UniProtKB; .
GOGO:0006953; P:acute-phase response; IEA:UniProtKB-KW; .
GOGO:0030277; P:maintenance of gastrointestinal epithelium; NAS:UniProtKB; .
GOGO:0019216; P:regulation of lipid metabolic process; NAS:UniProtKB; .
GOGO:0030162; P:regulation of proteolysis; IBA:RefGenome; .
InterProIPR000215; Protease_inhib_I4_serpin; .
InterProIPR023795; Protease_inhib_I4_serpin_CS; .
InterProIPR023796; Sepin_dom; .
PANTHERPTHR11461; Prot_inh_serpin; 1; .
PfamPF00079; Serpin; 1; .
SMARTSM00093; SERPIN; 1; .
SUPFAMSSF56574; Prot_inh_serpin; 1; .
PROSITEPS00284; SERPIN; 1; .



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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server