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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P01023




SWISS-2DPAGE:  P01023


P01023


General information about the entry
View entry in simple text format
Entry nameA2MG_HUMAN
Primary accession numberP01023
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onDecember 1, 2000 (version 1)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Alpha-2-macroglobulin; Short=Alpha-2-M; AltName: Full=C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; Flags: Precursor;.
Gene nameName=A2M
Synonyms=CPAMD5
ORFNames=FWP007
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=78094420; PubMed=271964; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Anderson N.G.
''''''High resolution two-dimensional electrophoresis of human plasma proteins'';'';''
Proc. Natl. Acad. Sci. U.S.A. 74(1):5421-5425(1977)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=5.48; Mw=184836
pI=5.45; Mw=183797
pI=5.51; Mw=183797
pI=5.98; Mw=183797
pI=5.41; Mw=182764
pI=5.53; Mw=182764
pI=5.57; Mw=183797
pI=5.63; Mw=182764
pI=5.66; Mw=182764
pI=5.70; Mw=182764
pI=5.73; Mw=182764
pI=5.77; Mw=182764
pI=5.80; Mw=182764
pI=5.85; Mw=182764
pI=5.88; Mw=182764
pI=5.91; Mw=182764
pI=5.94; Mw=183797
pI=5.39; Mw=181737
pI=5.60; Mw=181737
pI=5.33; Mw=181737
pI=5.35; Mw=181737
pI=5.37; Mw=181737

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=5.33; Mw=190595
pI=5.36; Mw=190595
pI=5.40; Mw=189274
pI=5.43; Mw=187962
pI=5.47; Mw=186659
pI=5.52; Mw=186659
pI=5.55; Mw=186659
pI=5.57; Mw=186659
pI=5.59; Mw=186659
pI=5.61; Mw=186659
pI=5.62; Mw=186659

NORMAL LEVEL:
1500-4000 MG/L.

PATHOLOGICAL LEVEL:
DECREASED DURING FIBRINOLYSIS AND ACUTE PANCREATITIS; INCREASED DURING NEPHROTIC SYNDROME, LIVER DISEASE, DIABETES MELLITUS AND INFLAMMATORY DISEASE.

POSITIONAL VARIANTS:
CLASSIFIED IN THE XM SYSTEM.

MAPPING (identification):
IMMUNOBLOTTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
DOSAC-COBS 2D-PAGEP01023; P01023.
DOSAC-COBS-2DPAGEP01023; A2MG_HUMAN.
Siena-2DPAGEP01023; A2MG_HUMAN.
UniProtKB/Swiss-ProtP01023; A2MG_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameA2MG_HUMAN
Primary accession numberP01023
Secondary accession number(s) Q13677 Q59F47 Q5QTS0 Q68DN2 Q6PIY3 Q6PN97
Sequence was last modified on October 5, 2010 (version 3)
Annotations were last modified on October 19, 2011 (version 145)
Name and origin of the protein
DescriptionRecName: Full=Alpha-2-macroglobulin; Short=Alpha-2-M; AltName: Full=C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; Flags: Precursor;
Gene nameName=A2M
Synonyms=CPAMD5
ORFNames=FWP007
Encoded onName=A2M; Synonyms=CPAMD5; ORFNames=FWP007
Keywords3D-structure; Bait region; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Isopeptide bond; Polymorphism; Protease inhibitor; Reference proteome; Secreted; Serine protease inhibitor; Signal; Thioester bond.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM11313; AAA51551.1; -; mRNA
EMBLAY591530; AAT02228.1; ALT_INIT; mRNA
EMBLAB209614; BAD92851.1; ALT_INIT; mRNA
EMBLCR749334; CAH18188.1; -; mRNA
EMBLAC007436; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLBC026246; AAH26246.1; -; mRNA
EMBLBC040071; AAH40071.1; -; mRNA
EMBLZ11711; CAA77774.1; -; Genomic_DNA
EMBLX68728; CAA48670.1; -; Genomic_DNA
EMBLX68729; CAA48670.1; JOINED; Genomic_DNA
EMBLM36501; AAA51552.1; -; mRNA
EMBLAF109189; AAQ13498.1; -; mRNA
IPIIPI00478003; -; .
PIRA94033; MAHU; .
RefSeqNP_000005.2; NM_000014.4; .
UniGeneHs.212838; -; .
PDB1BV8; NMR; -; A=1337-1474
PDB2P9R; X-ray; 2.30 A; A/B=126-227
PDBsum1BV8; -; .
PDBsum2P9R; -; .
ProteinModelPortalP01023; -; .
SMRP01023; 126-227; 727-954; 960-1270
1273-1474.
DIPDIP-1118N; -; .
IntActP01023; 39; .
MINTMINT-122288; -; .
STRINGP01023; -; .
MEROPSI39.001; -; .
PhosphoSiteP01023; -; .
SWISS-2DPAGEP01023; -; .
DOSAC-COBS-2DPAGEP01023; -; .
PeptideAtlasP01023; -; .
PRIDEP01023; -; .
EnsemblENST00000318602; ENSP00000323929; ENSG00000175899; .
GeneID2; -; .
KEGGhsa:2; -; .
UCSCuc001qvk.1; human; .
CTD2; -; .
GeneCardsGC12M009001; -; .
H-InvDBHIX0026392; -; .
HGNCHGNC:7; A2M; .
HPACAB017621; -; .
HPAHPA002265; -; .
MIM103950; gene+phenotype; .
neXtProtNX_P01023; -; .
PharmGKBPA24357; -; .
eggNOGprNOG17495; -; .
HOVERGENHBG000039; -; .
OMAICGNGRQ; -; .
OrthoDBEOG4H9XJN; -; .
Pathway_Interaction_DBil6_7pathway; IL6-mediated signaling events; .
ReactomeREACT_11044; Signaling by Rho GTPases; .
ReactomeREACT_604; Hemostasis; .
DrugBankDB00626; Bacitracin; .
DrugBankDB00102; Becaplermin; .
NextBio5; -; .
ArrayExpressP01023; -; .
BgeeP01023; -; .
CleanExHS_A2M; -; .
GenevestigatorP01023; -; .
GermOnlineENSG00000175899; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0005615; C:extracellular space; IEA:InterPro; .
GOGO:0031093; C:platelet alpha granule lumen; TAS:Reactome; .
GOGO:0019899; F:enzyme binding; IPI:UniProtKB; .
GOGO:0019966; F:interleukin-1 binding; IDA:UniProtKB; .
GOGO:0019959; F:interleukin-8 binding; IPI:UniProtKB; .
GOGO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB; .
GOGO:0043120; F:tumor necrosis factor binding; IDA:UniProtKB; .
GOGO:0007597; P:blood coagulation; intrinsic pathway; TAS:Reactome
GOGO:0001869; P:negative regulation of complement activation; lectin pathway; IDA:UniProtKB
GOGO:0030168; P:platelet activation; TAS:Reactome; .
GOGO:0002576; P:platelet degranulation; TAS:Reactome; .
GOGO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome; .
GOGO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome; .
InterProIPR009048; A-macroglobulin_rcpt-bd; .
InterProIPR011626; A2M_comp; .
InterProIPR002890; A2M_N; .
InterProIPR011625; A2M_N_2; .
InterProIPR001599; Macroglobln_a2; .
InterProIPR019742; MacrogloblnA2_CS; .
InterProIPR019565; MacrogloblnA2_thiol-ester-bond; .
InterProIPR008930; Terpenoid_cyclase/PrenylTrfase; .
InterProIPR010916; TonB_box_CS; .
Gene3DG3DSA:2.60.40.690; A-macroglobulin_rcpt-bd; 1; .
PfamPF00207; A2M; 1; .
PfamPF07678; A2M_comp; 1; .
PfamPF01835; A2M_N; 1; .
PfamPF07703; A2M_N_2; 1; .
PfamPF07677; A2M_recep; 1; .
PfamPF10569; Thiol-ester_cl; 1; .
SUPFAMSSF49410; AM_receptor_bind; 1; .
SUPFAMSSF48239; Terp_cyc_toroid; 1; .
PROSITEPS00477; ALPHA_2_MACROGLOBULIN; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server