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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P05055




SWISS-2DPAGE:  P05055


P05055


General information about the entry
View entry in simple text format
Entry namePNP_ECOLI
Primary accession numberP05055
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=Polyribonucleotide nucleotidyltransferase; EC=2.7.7.8; AltName: Full=Polynucleotide phosphorylase; Short=PNPase;.
Gene nameName=pnp
OrderedLocusNames=b3164, JW5851
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
MEDLINE=83270974; PubMed=6308410; [NCBI, ExPASy, EBI, Israel, Japan]
Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.
''''''Gene-protein index of Escherichia coli K-12'';'';''
Microbiol. Rev. 47(1):231-284(1983)
[3]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[4]   PROTEIN EXPRESSION
MEDLINE=87194592; PubMed=3553157; [NCBI, ExPASy, EBI, Israel, Japan]
Jones P.G., Vanbogelen R.A., Neidhardt F.C.
''''''Induction of proteins in response to low temperature in Escherichia coli'';'';''
J. Bacteriol. 169(1):2092-2095(1987)
[5]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[6]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMOTRIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.10; Mw=83954

EXPRESSION:
INCREASED DURING THE ADJUSTMENT OF E. COLI TO GROWTH AT 10C [4].

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.13; Mw=87327  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [5].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.16; Mw=80468  [identification data]
pI=5.15; Mw=78105  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [6].

MAPPING (identification):
SPOT 2D-001WD2: Peptide mass fingerprinting [6];
SPOT 2D-001WDK: Tandem mass spectrometry [6].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEC078.0; 6TH EDITION.
UniProtKB/Swiss-ProtP05055; PNP_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePNP_ECOLI
Primary accession numberP05055
Secondary accession number(s) P78109 Q2M946
Sequence was last modified on November 1, 1995 (version 3)
Annotations were last modified on October 19, 2011 (version 129)
Name and origin of the protein
DescriptionRecName: Full=Polyribonucleotide nucleotidyltransferase; EC=2.7.7.8; AltName: Full=Polynucleotide phosphorylase; Short=PNPase;
Gene nameName=pnp
OrderedLocusNames=b3164, JW5851
Encoded onName=pnp; OrderedLocusNames=b3164, JW5851
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotidyltransferase; Reference proteome; RNA-binding; Stress response; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ02638; AAA83905.1; -; Genomic_DNA
EMBLU18997; AAA57967.1; ALT_INIT; Genomic_DNA
EMBLU00096; AAC76198.2; -; Genomic_DNA
EMBLAP009048; BAE77210.1; ALT_INIT; Genomic_DNA
EMBLX00761; CAA25332.1; -; Genomic_DNA
EMBLM14425; AAA24596.1; -; Genomic_DNA
PIRH65106; H65106; .
RefSeqNP_417633.4; NC_000913.2; .
PDB1SRO; NMR; -; A=617-692
PDB3CDI; X-ray; 2.60 A; A=2-711
PDB3CDJ; X-ray; 2.80 A; A=2-547
PDB3H1C; X-ray; 3.57 A; A/B/C/G/I/K/M/O/R/T/V/X=1-549
PDBsum1SRO; -; .
PDBsum3CDI; -; .
PDBsum3CDJ; -; .
PDBsum3H1C; -; .
ProteinModelPortalP05055; -; .
SMRP05055; 1-692; .
DIPDIP-10522N; -; .
IntActP05055; 43; .
MINTMINT-244786; -; .
PhosSiteP05055; -; .
SWISS-2DPAGEP05055; -; .
ECO2DBASEC078.0; 6TH EDITION; .
PRIDEP05055; -; .
EnsemblBacteriaEBESCT00000002007; EBESCP00000002007; EBESCG00000001638; .
EnsemblBacteriaEBESCT00000002008; EBESCP00000002008; EBESCG00000001638; .
EnsemblBacteriaEBESCT00000002009; EBESCP00000002009; EBESCG00000001638; .
EnsemblBacteriaEBESCT00000002010; EBESCP00000002010; EBESCG00000001638; .
EnsemblBacteriaEBESCT00000015515; EBESCP00000014806; EBESCG00000014575; .
GeneID947672; -; .
GenomeReviewsAP009048_GR; JW5851; .
GenomeReviewsU00096_GR; b3164; .
KEGGecj:JW5851; -; .
KEGGeco:b3164; -; .
EchoBASEEB0736; -; .
EcoGeneEG10743; pnp; .
eggNOGCOG1185; -; .
GeneTreeEBGT00050000009530; -; .
HOGENOMHBG382411; -; .
OMAYGETVVL; -; .
ProtClustDBPRK11824; -; .
BioCycEcoCyc:EG10743-MONOMER; -; .
BioCycMetaCyc:EG10743-MONOMER; -; .
BRENDA2.7.7.8; 2026; .
GenevestigatorP05055; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0000175; F:3'-5'-exoribonuclease activity; IEA:InterPro; .
GOGO:0004654; F:polyribonucleotide nucleotidyltransferase activity; IEA:EC; .
GOGO:0003723; F:RNA binding; IEA:UniProtKB-KW; .
GOGO:0006402; P:mRNA catabolic process; IEA:InterPro; .
GOGO:0006950; P:response to stress; IEA:UniProtKB-KW; .
GOGO:0006396; P:RNA processing; IEA:InterPro; .
HAMAPMF_01595; PNPase; 1; -
InterProIPR001247; ExoRNase_PH_dom1; .
InterProIPR015847; ExoRNase_PH_dom2; .
InterProIPR004087; KH; .
InterProIPR009019; KH_prok-type; .
InterProIPR004088; KH_type_1; .
InterProIPR018111; KH_type_1_subgr; .
InterProIPR012340; NA-bd_OB-fold; .
InterProIPR016027; NA-bd_OB-fold-like; .
InterProIPR012162; PNPase; .
InterProIPR015848; PNPase_PH_RNA-bd_bac/org-type; .
InterProIPR003029; Rbsml_prot_S1_RNA-bd_dom; .
InterProIPR020568; Ribosomal_S5_D2-typ_fold; .
InterProIPR022967; RNA-binding_domain_S1; .
Gene3DG3DSA:2.40.50.140; OB_NA_bd_sub; 1; .
Gene3DG3DSA:1.10.10.400; PNPase_PH_RNA-bd_bac/org-type; 1; .
PANTHERPTHR11252; PNPase; 1; .
PfamPF00013; KH_1; 1; .
PfamPF03726; PNPase; 1; .
PfamPF01138; RNase_PH; 2; .
PfamPF03725; RNase_PH_C; 2; .
PfamPF00575; S1; 1; .
PIRSFPIRSF005499; PNPase; 1; .
SMARTSM00322; KH; 1; .
SMARTSM00316; S1; 1; .
SUPFAMSSF46915; 3_ExoRNase; 1; .
SUPFAMSSF55666; 3_ExoRNase; 2; .
SUPFAMSSF54814; KH_prok; 1; .
SUPFAMSSF50249; Nucleic_acid_OB; 1; .
SUPFAMSSF54211; Ribosomal_S5_D2-typ_fold; 2; .
TIGRFAMsTIGR03591; Polynuc_phos; 1; .
PROSITEPS50084; KH_TYPE_1; 1; .
PROSITEPS50126; S1; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server