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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P05317




SWISS-2DPAGE:  P05317


P05317


General information about the entry
View entry in simple text format
Entry nameRLA0_YEAST
Primary accession numberP05317
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=60S acidic ribosomal protein P0; Short=A0; AltName: Full=L10E;.
Gene nameName=RPP0
Synonyms=L10E, RPA0, RPL10E, RPLA0
OrderedLocusNames=YLR340W
ORFNames=L8300.8
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=95203288; PubMed=7895733; [NCBI, ExPASy, EBI, Israel, Japan]
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.
''''''Protein identifications for a Saccharomyces cerevisiae protein database'';'';''
Electrophoresis 15(1):1466-1486(1994)
Comments
  • SUBUNIT: P0 FORMS A PENTAMRTRIC COMPLEX BY INTERACTION WITH DIMERS OF P1 AND P2
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=4.78; Mw=32525

MAPPING (identification):
GEL MATCHING [1] AND FOUND IN PURIFIED RIBOSOMES BY GARRELS [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP05317; RLA0_YEAST.
YEPD9452.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRLA0_YEAST
Primary accession numberP05317
Secondary accession number(s) D6VYX8
Sequence was last modified on October 5, 2010 (version 2)
Annotations were last modified on October 19, 2011 (version 111)
Name and origin of the protein
DescriptionRecName: Full=60S acidic ribosomal protein P0; Short=A0; AltName: Full=L10E;
Gene nameName=RPP0
Synonyms=L10E, RPA0, RPL10E, RPLA0
OrderedLocusNames=YLR340W
ORFNames=L8300.8
Encoded onName=RPP0; Synonyms=L10E, RPA0, RPL10E, RPLA0; OrderedLocusNames=YLR340W; ORFNames=L8300.8
Keywords3D-structure; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; Ribonucleoprotein; Ribosomal protein.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX06959; CAA30029.1; -; mRNA
EMBLD00529; BAA00415.1; -; Genomic_DNA
EMBLX13328; CAA31703.1; -; Genomic_DNA
EMBLM26506; AAA34730.1; -; Genomic_DNA
EMBLM37326; AAA34729.1; -; Genomic_DNA
EMBLU19028; AAB67258.1; -; Genomic_DNA
EMBLBK006945; DAA09644.1; -; Genomic_DNA
PIRS51343; R5BY0E; .
RefSeqNP_013444.1; NM_001182229.1; .
PDB3IZS; EM; -; s=1-312
PDB3O5H; X-ray; 4.00 A; M=1-312
PDBsum3IZS; -; .
PDBsum3O5H; -; .
ProteinModelPortalP05317; -; .
SMRP05317; 3-120; 178-205; .
DIPDIP-1582N; -; .
IntActP05317; 258; .
MINTMINT-384116; -; .
STRINGP05317; -; .
SWISS-2DPAGEP05317; -; .
PeptideAtlasP05317; -; .
EnsemblFungiYLR340W; YLR340W; YLR340W; .
GeneID851052; -; .
KEGGsce:YLR340W; -; .
SGDS000004332; RPP0; .
eggNOGfuNOG04021; -; .
GeneTreeEFGT00050000004407; -; .
HOGENOMHBG601294; -; .
OMAYVFLFDL; -; .
OrthoDBEOG4Z39PT; -; .
PhylomeDBP05317; -; .
ArrayExpressP05317; -; .
GenevestigatorP05317; -; .
GermOnlineYLR340W; Saccharomyces cerevisiae; .
GOGO:0030686; C:90S preribosome; IDA:SGD; .
GOGO:0022625; C:cytosolic large ribosomal subunit; TAS:SGD; .
GOGO:0070180; F:LSU rRNA binding; IDA:SGD; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0003735; F:structural constituent of ribosome; TAS:SGD; .
GOGO:0002181; P:cytoplasmic translation; TAS:SGD; .
GOGO:0000027; P:ribosomal large subunit assembly; IMP:SGD; .
GOGO:0006414; P:translational elongation; IEA:InterPro; .
InterProIPR001813; Ribosomal_60S; .
InterProIPR001790; Ribosomal_L10/acidic_P0; .
PfamPF00428; Ribosomal_60s; 1; .
PfamPF00466; Ribosomal_L10; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server