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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P05791




SWISS-2DPAGE:  P05791


P05791


General information about the entry
View entry in simple text format
Entry nameILVD_ECOLI
Primary accession numberP05791
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 6)
Name and origin of the protein
DescriptionRecName: Full=Dihydroxy-acid dehydratase; Short=DAD; EC=4.2.1.9;.
Gene nameName=ilvD
OrderedLocusNames=b3771, JW5605
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.54; Mw=62065  [identification data]

EXPRESSION:
increase after benzoic acid treatment [1].

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP05791; ILVD_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameILVD_ECOLI
Primary accession numberP05791
Secondary accession number(s) Q2M880
Sequence was last modified on January 23, 2007 (version 5)
Annotations were last modified on October 19, 2011 (version 122)
Name and origin of the protein
DescriptionRecName: Full=Dihydroxy-acid dehydratase; Short=DAD; EC=4.2.1.9;
Gene nameName=ilvD
OrderedLocusNames=b3771, JW5605
Encoded onName=ilvD; OrderedLocusNames=b3771, JW5605
Keywords4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX04890; CAA28576.1; -; Genomic_DNA
EMBLM10313; AAB59053.1; -; Genomic_DNA
EMBLM87049; AAA67574.1; -; Genomic_DNA
EMBLU00096; AAT48208.1; -; Genomic_DNA
EMBLAP009048; BAE77526.1; -; Genomic_DNA
EMBLM32253; AAA24023.1; -; Genomic_DNA
EMBLX02413; CAA26263.1; -; Genomic_DNA
EMBLK03503; AAA24013.1; -; Genomic_DNA
PIRA27310; DWECDA; .
RefSeqYP_026248.1; NC_000913.2; .
ProteinModelPortalP05791; -; .
SMRP05791; 65-611; .
DIPDIP-10021N; -; .
IntActP05791; 1; .
MINTMINT-1302071; -; .
SWISS-2DPAGEP05791; -; .
EnsemblBacteriaEBESCT00000003396; EBESCP00000003396; EBESCG00000002784; .
EnsemblBacteriaEBESCT00000003397; EBESCP00000003397; EBESCG00000002784; .
EnsemblBacteriaEBESCT00000003398; EBESCP00000003398; EBESCG00000002784; .
EnsemblBacteriaEBESCT00000015012; EBESCP00000014303; EBESCG00000014072; .
GeneID948277; -; .
GenomeReviewsAP009048_GR; JW5605; .
GenomeReviewsU00096_GR; b3771; .
KEGGecj:JW5605; -; .
KEGGeco:b3771; -; .
EchoBASEEB0491; -; .
EcoGeneEG10496; ilvD; .
eggNOGCOG0129; -; .
GeneTreeEBGT00050000011250; -; .
HOGENOMHBG671001; -; .
OMASQDCRWP; -; .
ProtClustDBPRK12448; -; .
BioCycEcoCyc:DIHYDROXYACIDDEHYDRAT-MONOMER; -; .
BioCycMetaCyc:DIHYDROXYACIDDEHYDRAT-MONOMER; -; .
GenevestigatorP05791; -; .
GOGO:0051539; F:4 iron; 4 sulfur cluster binding; IEA:UniProtKB-KW
GOGO:0004160; F:dihydroxy-acid dehydratase activity; IEA:EC; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0009082; P:branched chain family amino acid biosynthetic process; IEA:UniProtKB-KW; .
HAMAPMF_00012; IlvD; 1; -
InterProIPR015928; Aconitase/3IPM_dehydase_swvl; .
InterProIPR004404; DihydroxyA_deHydtase; .
InterProIPR000581; DiOHA_6PGluconate_deHydtase; .
InterProIPR020558; DiOHA_6PGluconate_deHydtase_CS; .
PANTHERPTHR21000; ILVD_EDD_family; 1; .
PfamPF00920; ILVD_EDD; 1; .
SUPFAMSSF52016; Aconitase/3IPM_dehydase_swvl; 1; .
TIGRFAMsTIGR00110; IlvD; 1; .
PROSITEPS00886; ILVD_EDD_1; 1; .
PROSITEPS00887; ILVD_EDD_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server