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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P06151




SWISS-2DPAGE:  P06151


P06151


General information about the entry
View entry in simple text format
Entry nameLDHA_MOUSE
Primary accession numberP06151
integrated into SWISS-2DPAGE on June 30, 1999 (release 10)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=L-lactate dehydrogenase A chain; Short=LDH-A; EC=1.1.1.27; AltName: Full=LDH muscle subunit; Short=LDH-M;.
Gene nameName=Ldha
Synonyms=Ldh-1, Ldh1
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=6.98; Mw=34000  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=6.93; Mw=33645  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
REPRODUCTION-2DPAGEP06151; P06151.
UCD-2DPAGEP06151; LDHA_MOUSE.
UniProtKB/Swiss-ProtP06151; LDHA_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameLDHA_MOUSE
Primary accession numberP06151
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on September 21, 2011 (version 125)
Name and origin of the protein
DescriptionRecName: Full=L-lactate dehydrogenase A chain; Short=LDH-A; EC=1.1.1.27; AltName: Full=LDH muscle subunit; Short=LDH-M;
Gene nameName=Ldha
Synonyms=Ldh-1, Ldh1
Encoded onName=Ldha; Synonyms=Ldh-1, Ldh1
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLY00309; CAA68410.1; -; Genomic_DNA
EMBLX02520; CAA26360.1; -; Genomic_DNA
EMBLX02521; CAA26360.1; JOINED; Genomic_DNA
EMBLX02522; CAA26360.1; JOINED; Genomic_DNA
EMBLX02523; CAA26360.1; JOINED; Genomic_DNA
EMBLX02524; CAA26360.1; JOINED; Genomic_DNA
EMBLX02525; CAA26360.1; JOINED; Genomic_DNA
EMBLX02526; CAA26360.1; JOINED; Genomic_DNA
EMBLU13687; AAA21466.1; -; mRNA
EMBLX03753; CAA27387.1; -; Genomic_DNA
EMBLM17516; AAA39424.1; -; mRNA
IPIIPI00319994; -; .
PIRA25205; DEMSLM; .
PIRI48240; I48240; .
RefSeqNP_034829.1; NM_010699.2; .
UniGeneMm.29324; -; .
ProteinModelPortalP06151; -; .
SMRP06151; 2-332; .
IntActP06151; 8; .
STRINGP06151; -; .
PhosphoSiteP06151; -; .
SWISS-2DPAGEP06151; -; .
REPRODUCTION-2DPAGEP06151; -; .
UCD-2DPAGEP06151; -; .
PRIDEP06151; -; .
EnsemblENSMUST00000048209; ENSMUSP00000036386; ENSMUSG00000063229; .
GeneID16828; -; .
KEGGmmu:16828; -; .
CTD3939; -; .
MGIMGI:96759; Ldha; .
eggNOGroNOG06331; -; .
HOGENOMHBG566126; -; .
HOVERGENHBG000462; -; .
InParanoidP06151; -; .
OrthoDBEOG4RR6HR; -; .
NextBio290734; -; .
ArrayExpressP06151; -; .
BgeeP06151; -; .
CleanExMM_LDHA; -; .
GenevestigatorP06151; -; .
GermOnlineENSMUSG00000063229; Mus musculus; .
GOGO:0005829; C:cytosol; IDA:MGI; .
GOGO:0019861; C:flagellum; IDA:MGI; .
GOGO:0005739; C:mitochondrion; IDA:MGI; .
GOGO:0004459; F:L-lactate dehydrogenase activity; IDA:MGI; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0031668; P:cellular response to extracellular stimulus; IDA:MGI; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR011304; L-lactate_DH; .
InterProIPR018177; L-lactate_DH_AS; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
PRINTSPR00086; LLDHDRGNASE; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01771; L-LDH-NAD; 1; .
PROSITEPS00064; L_LDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server