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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P08839




SWISS-2DPAGE:  P08839


P08839


General information about the entry
View entry in simple text format
Entry namePT1_ECOLI
Primary accession numberP08839
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Phosphoenolpyruvate-protein phosphotransferase; EC=2.7.3.9; AltName: Full=Phosphotransferase system, enzyme I;.
Gene nameName=ptsI
OrderedLocusNames=b2416, JW2409
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.78; Mw=59836

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=4.91; Mw=63671  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [3].

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEB058.3; 6TH EDITION.
UniProtKB/Swiss-ProtP08839; PT1_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePT1_ECOLI
Primary accession numberP08839
Sequence was last modified on November 1, 1988 (version 1)
Annotations were last modified on October 19, 2011 (version 131)
Name and origin of the protein
DescriptionRecName: Full=Phosphoenolpyruvate-protein phosphotransferase; EC=2.7.3.9; AltName: Full=Phosphotransferase system, enzyme I;
Gene nameName=ptsI
OrderedLocusNames=b2416, JW2409
Encoded onName=ptsI; OrderedLocusNames=b2416, JW2409
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Magnesium; Metal-binding; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ02796; AAA24441.1; -; Genomic_DNA
EMBLM10425; AAA24439.1; -; Genomic_DNA
EMBLU00096; AAC75469.1; -; Genomic_DNA
EMBLAP009048; BAA16290.1; -; Genomic_DNA
EMBLM21994; AAA24385.1; -; Genomic_DNA
EMBLM21451; AAA23656.1; -; Genomic_DNA
PIRB29785; WQECPI; .
RefSeqNP_416911.1; NC_000913.2; .
PDB1EZA; NMR; -; A=1-258
PDB1EZB; NMR; -; A=1-258
PDB1EZC; NMR; -; A=1-258
PDB1EZD; NMR; -; A=1-258
PDB1ZYM; X-ray; 2.50 A; A/B=1-258
PDB2EZA; NMR; -; A=1-258
PDB2EZB; NMR; -; A=1-258
PDB2EZC; NMR; -; A=1-258
PDB2HWG; X-ray; 2.70 A; A/B=1-575
PDB2KX9; Other; -; A/B=1-573
PDB2L5H; Other; -; A/B=1-573
PDB2XDF; Other; -; A/B=1-573
PDB3EZA; NMR; -; A=1-249
PDB3EZB; NMR; -; A=1-258
PDB3EZE; NMR; -; A=1-258
PDBsum1EZA; -; .
PDBsum1EZB; -; .
PDBsum1EZC; -; .
PDBsum1EZD; -; .
PDBsum1ZYM; -; .
PDBsum2EZA; -; .
PDBsum2EZB; -; .
PDBsum2EZC; -; .
PDBsum2HWG; -; .
PDBsum2KX9; -; .
PDBsum2L5H; -; .
PDBsum2XDF; -; .
PDBsum3EZA; -; .
PDBsum3EZB; -; .
PDBsum3EZE; -; .
ProteinModelPortalP08839; -; .
SMRP08839; 1-573; .
DIPDIP-10603N; -; .
IntActP08839; 11; .
MINTMINT-346878; -; .
TCDB8.A.7.1.1; phosphotransferase system enzyme I (EI) family; .
PhosSiteP08839; -; .
SWISS-2DPAGEP08839; -; .
ECO2DBASEB058.3; 6TH EDITION; .
EnsemblBacteriaEBESCT00000003532; EBESCP00000003532; EBESCG00000002889; .
EnsemblBacteriaEBESCT00000003533; EBESCP00000003533; EBESCG00000002889; .
EnsemblBacteriaEBESCT00000003534; EBESCP00000003534; EBESCG00000002889; .
EnsemblBacteriaEBESCT00000003535; EBESCP00000003535; EBESCG00000002889; .
EnsemblBacteriaEBESCT00000003536; EBESCP00000003536; EBESCG00000002889; .
EnsemblBacteriaEBESCT00000015552; EBESCP00000014843; EBESCG00000014612; .
GeneID946879; -; .
GenomeReviewsAP009048_GR; JW2409; .
GenomeReviewsU00096_GR; b2416; .
KEGGecj:JW2409; -; .
KEGGeco:b2416; -; .
EchoBASEEB0782; -; .
EcoGeneEG10789; ptsI; .
eggNOGCOG1080; -; .
GeneTreeEBGT00050000009028; -; .
HOGENOMHBG456539; -; .
OMAAAIFARS; -; .
ProtClustDBPRK11177; -; .
BioCycEcoCyc:PTSI-MONOMER; -; .
BioCycMetaCyc:PTSI-MONOMER; -; .
BRENDA2.7.3.9; 2026; .
GenevestigatorP08839; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016301; F:kinase activity; IEA:UniProtKB-KW; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:EC; .
GOGO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro; .
GOGO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW; .
InterProIPR008279; PEP-util_enz_mobile_dom; .
InterProIPR018274; PEP_util_AS; .
InterProIPR000121; PEP_util_C; .
InterProIPR023151; PEP_util_CS; .
InterProIPR006318; PEP_util_enz; .
InterProIPR024692; PTS_enz_I; .
InterProIPR008731; PTS_PEP_utilis_N; .
InterProIPR015813; Pyrv/PenolPyrv_Kinase; .
Gene3DG3DSA:3.50.30.10; PEP_mobile; 1; .
Gene3DG3DSA:1.10.274.10; PTS_PEP_utilis_N; 1; .
Gene3DG3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1; .
PANTHERPTHR22931:SF10; PTHR22931:SF10; 1; .
PfamPF05524; PEP-utilisers_N; 1; .
PfamPF00391; PEP-utilizers; 1; .
PfamPF02896; PEP-utilizers_C; 1; .
PIRSFPIRSF000732; PTS_enzyme_I; 1; .
PRINTSPR01736; PHPHTRNFRASE; .
SUPFAMSSF47831; PEP-utilisers_N; 1; .
SUPFAMSSF52009; PEP_mobile; 1; .
SUPFAMSSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMsTIGR01417; PTS_I_fam; 1; .
PROSITEPS00742; PEP_ENZYMES_2; 1; .
PROSITEPS00370; PEP_ENZYMES_PHOS_SITE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server