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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6Y8




SWISS-2DPAGE:  P0A6Y8


P0A6Y8


General information about the entry
View entry in simple text format
Entry nameDNAK_ECOLI
Primary accession numberP0A6Y8
Secondary accession number(s) P04475
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 6)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Chaperone protein DnaK; AltName: Full=HSP70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=Heat shock protein 70;.
Gene nameName=dnaK
Synonyms=groP, grpF, seg
OrderedLocusNames=b0014, JW0013
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
MEDLINE=83270974; PubMed=6308410; [NCBI, ExPASy, EBI, Israel, Japan]
Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.
''''''Gene-protein index of Escherichia coli K-12'';'';''
Microbiol. Rev. 47(1):231-284(1983)
[3]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[4]   PROTEIN EXPRESSION
MEDLINE=94042879; PubMed=8226654; [NCBI, ExPASy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C.
''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';''
J. Bacteriol. 175(1):7105-7108(1993)
[5]   PROTEIN EXPRESSION
MEDLINE=87083376; PubMed=3539918; [NCBI, ExPASy, EBI, Israel, Japan]
Vanbogelen R.A., Kelley P.M., Neidhardt F.C.
''''''Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli'';'';''
J. Bacteriol. 169(1):26-32(1987)
[6]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[7]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.80; Mw=69647

EXPRESSION:
INCREASED FOLLOWING EXPOSURE TO DNP [4]; WAS INDUCED BY TEMPERATURE SHIFTS, ETHANOL, NALIDIXIC ACID AND PUROMYCIN [5].

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].



ECOLI4-5 {Escherichia coli(4-5)}
Escherichia coli
ECOLI4-5
  map experimental info
  protein estimated location
 
ECOLI4-5

MAP LOCATIONS:
pI=4.98; Mw=70671  [identification data]
pI=4.98; Mw=67488  [identification data]
pI=4.96; Mw=61267  [identification data]
pI=4.97; Mw=61125  [identification data]
pI=4.95; Mw=60700  [identification data]
pI=4.95; Mw=60559  [identification data]
pI=4.96; Mw=60841
pI=4.95; Mw=60700  [identification data]
pI=4.48; Mw=59582  [identification data]
pI=4.93; Mw=52435  [identification data]
pI=5.00; Mw=44877  [identification data]

MAPPING (identification):
SPOTS 1HFT, 1HFV, 1HFW, 1HF6, 1HFC, 1HFS, 1HFY, 1HFZ, 1HH6, 1HIJ: Peptide mass fingerprinting [6];
SPOT 2D-001HFW: MICROSEQUENCING (XFQDE) [6]; SPOTS 1HFW, 1HFX, 1HH6: GEL MATCHING WITH MASTER ECOLI4.5-5.5 [6].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=4.96; Mw=60487  [identification data]
pI=4.97; Mw=60010  [identification data]
pI=4.95; Mw=53154  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6];
SPOT 2D-0014TQ: MICROSEQUENCING (XFQD) [6].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=4.92; Mw=74532  [identification data]
pI=4.91; Mw=74850  [identification data]
pI=4.94; Mw=73587  [identification data]

EXPRESSION:
increase after benzoic acid treatment [7].

MAPPING (identification):
SPOTS 1WDV, 1WE2: Peptide mass fingerprinting [7];
SPOT 2D-001WDY: Tandem mass spectrometry [7].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
2DBase-EcoliP0A6Y8; DNAK_ECOLI.
ECO2DBASEB066.0; 6TH EDITION.
UniProtKB/Swiss-ProtP0A6Y8; DNAK_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDNAK_ECOLI
Primary accession numberP0A6Y8
Secondary accession number(s) P04475
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 79)
Name and origin of the protein
DescriptionRecName: Full=Chaperone protein DnaK; AltName: Full=HSP70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=Heat shock protein 70;
Gene nameName=dnaK
Synonyms=groP, grpF, seg
OrderedLocusNames=b0014, JW0013
Encoded onName=dnaK; Synonyms=groP, grpF, seg; OrderedLocusNames=b0014, JW0013
Keywords3D-structure; Acetylation; ATP-binding; Cell inner membrane; Cell membrane; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; DNA replication; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK01298; AAA23694.1; -; Genomic_DNA
EMBLU00096; AAC73125.1; -; Genomic_DNA
EMBLAP009048; BAB96589.1; -; Genomic_DNA
EMBLD10765; BAA01595.1; -; Genomic_DNA
EMBLM12565; AAA23692.1; -; Genomic_DNA
PIRA03311; IQECDK; .
RefSeqNP_414555.1; NC_000913.2; .
PDB1BPR; NMR; -; A=386-561
PDB1DG4; NMR; -; A=393-507
PDB1DKG; X-ray; 2.80 A; D=1-383
PDB1DKX; X-ray; 2.00 A; A=389-607
PDB1DKY; X-ray; 2.80 A; A/B=389-607
PDB1DKZ; X-ray; 2.00 A; A=389-607
PDB1Q5L; NMR; -; A=393-506
PDB2BPR; NMR; -; A=386-561
PDB2KHO; NMR; -; A=1-605
PDB3DPO; X-ray; 2.10 A; A/B=389-607
PDB3DPP; X-ray; 2.50 A; A/B=389-607
PDB3DPQ; X-ray; 2.60 A; A/B/E/F=389-607
PDB3QNJ; X-ray; 2.28 A; A/B=389-607
PDBsum1BPR; -; .
PDBsum1DG4; -; .
PDBsum1DKG; -; .
PDBsum1DKX; -; .
PDBsum1DKY; -; .
PDBsum1DKZ; -; .
PDBsum1Q5L; -; .
PDBsum2BPR; -; .
PDBsum2KHO; -; .
PDBsum3DPO; -; .
PDBsum3DPP; -; .
PDBsum3DPQ; -; .
PDBsum3QNJ; -; .
ProteinModelPortalP0A6Y8; -; .
SMRP0A6Y8; 4-603; .
DIPDIP-35751N; -; .
IntActP0A6Y8; 365; .
MINTMINT-7259066; -; .
TCDB1.A.33.1.2; cation channel-forming heat shock protein-70 (Hsp70) family; .
PhosSiteP0A6Y8; -; .
SWISS-2DPAGEP0A6Y8; -; .
2DBase-EcoliP0A6Y8; -; .
ECO2DBASEB066.0; 6TH EDITION; .
PRIDEP0A6Y8; -; .
EnsemblBacteriaEBESCT00000004249; EBESCP00000004249; EBESCG00000003466; .
EnsemblBacteriaEBESCT00000004250; EBESCP00000004250; EBESCG00000003466; .
EnsemblBacteriaEBESCT00000004251; EBESCP00000004251; EBESCG00000003466; .
EnsemblBacteriaEBESCT00000014659; EBESCP00000013950; EBESCG00000013720; .
GeneID944750; -; .
GenomeReviewsAP009048_GR; JW0013; .
GenomeReviewsU00096_GR; b0014; .
KEGGecj:JW0013; -; .
KEGGeco:b0014; -; .
EchoBASEEB0237; -; .
EcoGeneEG10241; dnaK; .
eggNOGCOG0443; -; .
GeneTreeEBGT00050000009550; -; .
HOGENOMHBG334976; -; .
OMAYITMDAT; -; .
ProtClustDBPRK00290; -; .
BioCycEcoCyc:EG10241-MONOMER; -; .
GenevestigatorP0A6Y8; -; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; IEA:InterPro; .
GOGO:0006260; P:DNA replication; IEA:UniProtKB-KW; .
GOGO:0006457; P:protein folding; IEA:InterPro; .
GOGO:0006950; P:response to stress; IEA:UniProtKB-KW; .
HAMAPMF_00332; DnaK; 1; -
InterProIPR012725; Chaperone_DnaK; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
TIGRFAMsTIGR02350; Prok_dnaK; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server