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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A786




SWISS-2DPAGE:  P0A786


P0A786


General information about the entry
View entry in simple text format
Entry namePYRB_ECOLI
Primary accession numberP0A786
Secondary accession number(s) P00479
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Aspartate carbamoyltransferase catalytic chain; EC=2.1.3.2; AltName: Full=Aspartate transcarbamylase; Short=ATCase;.
Gene nameName=pyrB
OrderedLocusNames=b4245, JW4204
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
MEDLINE=83270974; PubMed=6308410; [NCBI, ExPASy, EBI, Israel, Japan]
Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.
''''''Gene-protein index of Escherichia coli K-12'';'';''
Microbiol. Rev. 47(1):231-284(1983)
[3]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
Comments
  • SUBUNIT: CONTAINS SIX CATALYTIC AND SIX REGULATORY CHAINS
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.70; Mw=93664
pI=6.13; Mw=35391

MAPPING (identification):
MICROSEQUENCE [1] AND/OR AMINO ACID COMPOSITION ANALYSIS AND IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEH031.3; 6TH EDITION.
UniProtKB/Swiss-ProtP0A786; PYRB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePYRB_ECOLI
Primary accession numberP0A786
Secondary accession number(s) P00479 Q2M662 Q47555 Q47557
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 72)
Name and origin of the protein
DescriptionRecName: Full=Aspartate carbamoyltransferase catalytic chain; EC=2.1.3.2; AltName: Full=Aspartate transcarbamylase; Short=ATCase;
Gene nameName=pyrB
OrderedLocusNames=b4245, JW4204
Encoded onName=pyrB; OrderedLocusNames=b4245, JW4204
Keywords3D-structure; Complete proteome; Direct protein sequencing; Pyrimidine biosynthesis; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01670; AAA24474.1; -; Genomic_DNA
EMBLK01472; AAA24476.1; -; Genomic_DNA
EMBLU14003; AAA97142.1; -; Genomic_DNA
EMBLU00096; AAC77202.1; -; Genomic_DNA
EMBLAP009048; BAE78244.1; -; Genomic_DNA
EMBLM10743; AAA24479.1; -; Genomic_DNA
EMBLM60508; AAA24481.1; -; Genomic_DNA
PIRH65236; DTECC; .
RefSeqNP_418666.1; NC_000913.2; .
PDB1ACM; X-ray; 2.80 A; A/C=2-310
PDB1AT1; X-ray; 2.80 A; A/C=2-310
PDB1D09; X-ray; 2.10 A; A/C=2-311
PDB1EKX; X-ray; 1.95 A; A/B/C=1-311
PDB1EZZ; X-ray; 2.70 A; A/C=2-310
PDB1F1B; X-ray; 2.30 A; A/C=2-310
PDB1GQ3; X-ray; 2.01 A; A/B/C=2-311
PDB1I5O; X-ray; 2.80 A; A/C=2-310
PDB1NBE; X-ray; 2.60 A; A/C=2-310
PDB1Q95; X-ray; 2.46 A; A/B/C/D/E/F=2-310
PDB1R0B; X-ray; 2.90 A; A/B/C/D/E/F=2-310
PDB1R0C; X-ray; 2.37 A; A/G=2-310
PDB1RAA; X-ray; 2.50 A; A/C=2-310
PDB1RAB; X-ray; 2.50 A; A/C=2-310
PDB1RAC; X-ray; 2.50 A; A/C=2-310
PDB1RAD; X-ray; 2.50 A; A/C=2-310
PDB1RAE; X-ray; 2.50 A; A/C=2-310
PDB1RAF; X-ray; 2.50 A; A/C=2-310
PDB1RAG; X-ray; 2.50 A; A/C=2-310
PDB1RAH; X-ray; 2.50 A; A/C=2-310
PDB1RAI; X-ray; 2.50 A; A/C=2-310
PDB1SKU; X-ray; 2.60 A; A/C=2-310
PDB1TTH; X-ray; 2.80 A; A/C=2-310
PDB1TU0; X-ray; 2.55 A; A/C=2-310
PDB1TUG; X-ray; 2.10 A; A/C=2-310
PDB1XJW; X-ray; 2.71 A; A/C=2-310
PDB1ZA1; X-ray; 2.20 A; A/C=1-311
PDB1ZA2; X-ray; 2.50 A; A/C=1-311
PDB2A0F; X-ray; 2.90 A; A/C=2-310
PDB2AIR; X-ray; 2.00 A; A/G=2-310
PDB2AT1; X-ray; 2.80 A; A/C=2-310
PDB2ATC; X-ray; 3.00 A; A=2-310
PDB2FZC; X-ray; 2.10 A; A/C=2-310
PDB2FZG; X-ray; 2.25 A; A/C=2-310
PDB2FZK; X-ray; 2.50 A; A/C=2-310
PDB2H3E; X-ray; 2.30 A; A/C=2-310
PDB2HSE; X-ray; 2.60 A; A/C=2-310
PDB2IPO; X-ray; 2.60 A; A/C=2-310
PDB2QG9; X-ray; 2.70 A; A/C=2-311
PDB2QGF; X-ray; 2.20 A; A/C=2-311
PDB3AT1; X-ray; 2.80 A; A/C=2-310
PDB3CSU; X-ray; 1.88 A; A/B/C=2-311
PDB3D7S; X-ray; 2.80 A; A/C=2-311
PDB3MPU; X-ray; 2.86 A; A/C/E=2-311
PDB3NPM; X-ray; 2.10 A; A=2-311
PDB4AT1; X-ray; 2.60 A; A/C=2-311
PDB5AT1; X-ray; 2.60 A; A/C=2-311
PDB6AT1; X-ray; 2.60 A; A/C=2-311
PDB7AT1; X-ray; 2.80 A; A/C=2-311
PDB8AT1; X-ray; 2.80 A; A/C=2-311
PDB8ATC; X-ray; 2.50 A; A/C=2-310
PDB9ATC; X-ray; 2.40 A; A=2-310
PDBsum1ACM; -; .
PDBsum1AT1; -; .
PDBsum1D09; -; .
PDBsum1EKX; -; .
PDBsum1EZZ; -; .
PDBsum1F1B; -; .
PDBsum1GQ3; -; .
PDBsum1I5O; -; .
PDBsum1NBE; -; .
PDBsum1Q95; -; .
PDBsum1R0B; -; .
PDBsum1R0C; -; .
PDBsum1RAA; -; .
PDBsum1RAB; -; .
PDBsum1RAC; -; .
PDBsum1RAD; -; .
PDBsum1RAE; -; .
PDBsum1RAF; -; .
PDBsum1RAG; -; .
PDBsum1RAH; -; .
PDBsum1RAI; -; .
PDBsum1SKU; -; .
PDBsum1TTH; -; .
PDBsum1TU0; -; .
PDBsum1TUG; -; .
PDBsum1XJW; -; .
PDBsum1ZA1; -; .
PDBsum1ZA2; -; .
PDBsum2A0F; -; .
PDBsum2AIR; -; .
PDBsum2AT1; -; .
PDBsum2ATC; -; .
PDBsum2FZC; -; .
PDBsum2FZG; -; .
PDBsum2FZK; -; .
PDBsum2H3E; -; .
PDBsum2HSE; -; .
PDBsum2IPO; -; .
PDBsum2QG9; -; .
PDBsum2QGF; -; .
PDBsum3AT1; -; .
PDBsum3CSU; -; .
PDBsum3D7S; -; .
PDBsum3MPU; -; .
PDBsum3NPM; -; .
PDBsum4AT1; -; .
PDBsum5AT1; -; .
PDBsum6AT1; -; .
PDBsum7AT1; -; .
PDBsum8AT1; -; .
PDBsum8ATC; -; .
PDBsum9ATC; -; .
ProteinModelPortalP0A786; -; .
SMRP0A786; 2-311; .
IntActP0A786; 3; .
SWISS-2DPAGEP0A786; -; .
ECO2DBASEH031.3; 6TH EDITION; .
EnsemblBacteriaEBESCT00000003057; EBESCP00000003057; EBESCG00000002510; .
EnsemblBacteriaEBESCT00000003058; EBESCP00000003058; EBESCG00000002510; .
EnsemblBacteriaEBESCT00000018213; EBESCP00000017504; EBESCG00000017268; .
GeneID948767; -; .
GenomeReviewsAP009048_GR; JW4204; .
GenomeReviewsU00096_GR; b4245; .
KEGGecj:JW4204; -; .
KEGGeco:b4245; -; .
EchoBASEEB0798; -; .
EcoGeneEG10805; pyrB; .
eggNOGCOG0540; -; .
GeneTreeEBGT00050000009122; -; .
HOGENOMHBG579429; -; .
OMAADRYENT; -; .
ProtClustDBPRK00856; -; .
BioCycEcoCyc:ASPCARBCAT-MONOMER; -; .
BioCycMetaCyc:ASPCARBCAT-MONOMER; -; .
GenevestigatorP0A786; -; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0016597; F:amino acid binding; IEA:InterPro; .
GOGO:0004070; F:aspartate carbamoyltransferase activity; IEA:EC; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0006207; P:'de novo' pyrimidine base biosynthetic process; IEA:InterPro; .
GOGO:0006520; P:cellular amino acid metabolic process; IEA:InterPro; .
GOGO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:UniProtKB-KW; .
HAMAPMF_00001; Asp_carb_tr; 1; -
InterProIPR006132; Asp/Orn_carbamoyltranf_P-bd; .
InterProIPR006130; Asp/Orn_carbamoylTrfase; .
InterProIPR006131; Asp_carbamoyltransf_Asp/Orn-bd; .
InterProIPR002082; Asp_carbamoyltransf_euk; .
PfamPF00185; OTCace; 1; .
PfamPF02729; OTCace_N; 1; .
PRINTSPR00100; AOTCASE; .
PRINTSPR00101; ATCASE; .
SUPFAMSSF53671; Asp/Orn_carbamoyltranf; 1; .
TIGRFAMsTIGR00670; Asp_carb_tr; 1; .
PROSITEPS00097; CARBAMOYLTRANSFERASE; 1; .



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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server