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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A799




SWISS-2DPAGE:  P0A799


P0A799


General information about the entry
View entry in simple text format
Entry namePGK_ECOLI
Primary accession numberP0A799
Secondary accession number(s) P11665
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 5)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Phosphoglycerate kinase; EC=2.7.2.3;.
Gene nameName=pgk
OrderedLocusNames=b2926, JW2893
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[5]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: MONOMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.07; Mw=42043  [identification data]
pI=5.02; Mw=41877  [identification data]
pI=5.00; Mw=41546

MAPPING (identification):
SPOT 2D-000KZ9: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]; SPOTS 2D-000KZD, 2D-000KZS: AMINO ACID COMPOSITION AND SEQUENCE TAG (SVIK) [3].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.15; Mw=40464  [identification data]
pI=5.14; Mw=39816

MAPPING (identification):
MICROSEQUENCING (XVIK AND XVFI) [4];
SPOT 2D-00151K: MASS FINGERPRINTING [4].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.30; Mw=52681  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [5].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A799; PGK_ECOLI.
UniProtKB/Swiss-ProtP0A799; PGK_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePGK_ECOLI
Primary accession numberP0A799
Secondary accession number(s) P11665 Q2M9R6
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 61)
Name and origin of the protein
DescriptionRecName: Full=Phosphoglycerate kinase; EC=2.7.2.3;
Gene nameName=pgk
OrderedLocusNames=b2926, JW2893
Encoded onName=pgk; OrderedLocusNames=b2926, JW2893
Keywords3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX14436; CAA32604.1; -; Genomic_DNA
EMBLU28377; AAA69093.1; -; Genomic_DNA
EMBLU00096; AAC75963.1; -; Genomic_DNA
EMBLAP009048; BAE76990.1; -; Genomic_DNA
PIRS04733; TVECG; .
RefSeqNP_417401.1; NC_000913.2; .
PDB1ZMR; X-ray; 2.40 A; A=1-387
PDBsum1ZMR; -; .
ProteinModelPortalP0A799; -; .
SMRP0A799; 2-387; .
DIPDIP-36163N; -; .
IntActP0A799; 14; .
MINTMINT-1229247; -; .
PhosSiteP0A799; -; .
SWISS-2DPAGEP0A799; -; .
2DBase-EcoliP0A799; -; .
EnsemblBacteriaEBESCT00000004517; EBESCP00000004517; EBESCG00000003689; .
EnsemblBacteriaEBESCT00000004518; EBESCP00000004518; EBESCG00000003689; .
EnsemblBacteriaEBESCT00000004519; EBESCP00000004519; EBESCG00000003689; .
EnsemblBacteriaEBESCT00000004520; EBESCP00000004520; EBESCG00000003689; .
EnsemblBacteriaEBESCT00000014464; EBESCP00000013755; EBESCG00000013525; .
GeneID947414; -; .
GenomeReviewsAP009048_GR; JW2893; .
GenomeReviewsU00096_GR; b2926; .
KEGGecj:JW2893; -; .
KEGGeco:b2926; -; .
EchoBASEEB0697; -; .
EcoGeneEG10703; pgk; .
eggNOGCOG0126; -; .
GeneTreeEBGT00050000011026; -; .
HOGENOMHBG453500; -; .
OMADCIGDEV; -; .
ProtClustDBPRK00073; -; .
BioCycEcoCyc:PGK; -; .
BioCycMetaCyc:PGK; -; .
GenevestigatorP0A799; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004618; F:phosphoglycerate kinase activity; IEA:EC; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
HAMAPMF_00145; Phosphoglyc_kinase; 1; -
InterProIPR001576; Phosphoglycerate_kinase; .
InterProIPR015901; Phosphoglycerate_kinase_C; .
InterProIPR015911; Phosphoglycerate_kinase_CS; .
InterProIPR015824; Phosphoglycerate_kinase_N; .
Gene3DG3DSA:3.40.50.1270; Phosphoglycerate_kinase_C; 1; .
Gene3DG3DSA:3.40.50.1260; Phosphoglycerate_kinase_N; 1; .
PANTHERPTHR11406; PGK; 1; .
PfamPF00162; PGK; 1; .
PIRSFPIRSF000724; Pgk; 1; .
PRINTSPR00477; PHGLYCKINASE; .
SUPFAMSSF53748; PGK; 1; .
PROSITEPS00111; PGLYCERATE_KINASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server