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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A7A9




SWISS-2DPAGE:  P0A7A9


P0A7A9


General information about the entry
View entry in simple text format
Entry nameIPYR_ECOLI
Primary accession numberP0A7A9
Secondary accession number(s) P17288
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Inorganic pyrophosphatase; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase; Short=PPase;.
Gene nameName=ppa
OrderedLocusNames=b4226, JW4185
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[2]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[3]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.01; Mw=21579

MAPPING (identification):
AMINO ACID COMPOSITION AND SEQUENCE TAG (SLLN) [1].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.06; Mw=21653  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2], and microsequencing (SLLN) [2].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.00; Mw=22748  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A7A9; IPYR_ECOLI.
UniProtKB/Swiss-ProtP0A7A9; IPYR_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameIPYR_ECOLI
Primary accession numberP0A7A9
Secondary accession number(s) P17288 Q2M679
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 62)
Name and origin of the protein
DescriptionRecName: Full=Inorganic pyrophosphatase; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase; Short=PPase;
Gene nameName=ppa
OrderedLocusNames=b4226, JW4185
Encoded onName=ppa; OrderedLocusNames=b4226, JW4185
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM23550; AAB88583.1; -; Unassigned_DNA
EMBLU14003; AAA97123.1; -; Genomic_DNA
EMBLU00096; AAC77183.1; -; Genomic_DNA
EMBLAP009048; BAE78227.1; -; Genomic_DNA
PIRA27648; PWEC; .
RefSeqNP_418647.1; NC_000913.2; .
PDB1FAJ; X-ray; 2.15 A; A=2-176
PDB1I40; X-ray; 1.10 A; A=2-175
PDB1I6T; X-ray; 1.20 A; A=2-175
PDB1IGP; X-ray; 2.20 A; A=2-175
PDB1INO; X-ray; 2.20 A; A=2-175
PDB1IPW; X-ray; 2.30 A; A/B=2-176
PDB1JFD; X-ray; 2.20 A; A/B=2-176
PDB1MJW; X-ray; 1.95 A; A/B=2-175
PDB1MJX; X-ray; 2.15 A; A/B=2-175
PDB1MJY; X-ray; 2.10 A; A/B=2-175
PDB1MJZ; X-ray; 2.20 A; A=2-175
PDB1OBW; X-ray; 1.90 A; A/B/C=2-176
PDB2AU6; X-ray; 1.20 A; A=2-175
PDB2AU7; X-ray; 1.05 A; A=2-175
PDB2AU8; X-ray; 1.65 A; A=2-175
PDB2AU9; X-ray; 1.30 A; A=2-175
PDB2AUU; X-ray; 1.22 A; A=2-175
PDB2EIP; X-ray; 2.20 A; A/B=2-176
PDBsum1FAJ; -; .
PDBsum1I40; -; .
PDBsum1I6T; -; .
PDBsum1IGP; -; .
PDBsum1INO; -; .
PDBsum1IPW; -; .
PDBsum1JFD; -; .
PDBsum1MJW; -; .
PDBsum1MJX; -; .
PDBsum1MJY; -; .
PDBsum1MJZ; -; .
PDBsum1OBW; -; .
PDBsum2AU6; -; .
PDBsum2AU7; -; .
PDBsum2AU8; -; .
PDBsum2AU9; -; .
PDBsum2AUU; -; .
PDBsum2EIP; -; .
ProteinModelPortalP0A7A9; -; .
SMRP0A7A9; 2-176; .
DIPDIP-36217N; -; .
IntActP0A7A9; 36; .
MINTMINT-1226708; -; .
PhosSiteP0A7A9; -; .
SWISS-2DPAGEP0A7A9; -; .
2DBase-EcoliP0A7A9; -; .
EnsemblBacteriaEBESCT00000003037; EBESCP00000003037; EBESCG00000002490; .
EnsemblBacteriaEBESCT00000015331; EBESCP00000014622; EBESCG00000014391; .
GeneID948748; -; .
GenomeReviewsAP009048_GR; JW4185; .
GenomeReviewsU00096_GR; b4226; .
KEGGecj:JW4185; -; .
KEGGeco:b4226; -; .
EchoBASEEB0748; -; .
EcoGeneEG10755; ppa; .
eggNOGCOG0221; -; .
GeneTreeEBGT00050000009133; -; .
HOGENOMHBG529150; -; .
OMAAMVIMEE; -; .
ProtClustDBPRK01250; -; .
BioCycEcoCyc:INORGPYROPHOSPHAT-MONOMER; -; .
BioCycMetaCyc:INORGPYROPHOSPHAT-MONOMER; -; .
GenevestigatorP0A7A9; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0004427; F:inorganic diphosphatase activity; IEA:EC; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0008270; F:zinc ion binding; IDA:EcoliWiki; .
GOGO:0006796; P:phosphate-containing compound metabolic process; IEA:InterPro; .
HAMAPMF_00209; Inorganic_PPase; 1; -
InterProIPR008162; Pyrophosphatase; .
Gene3DG3DSA:3.90.80.10; Pyrophosphatase; 1; .
PANTHERPTHR10286; Pyrophosphatase; 1; .
PfamPF00719; Pyrophosphatase; 1; .
SUPFAMSSF50324; Pyrophosphatase; 1; .
PROSITEPS00387; PPASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server