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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis


Searching in 'SWISS-2DPAGE' for entry matching: P0A7D7




SWISS-2DPAGE:  P0A7D7

P0A7D7


General information about the entry
View entry in simple text format
Entry namePUR7_ECOLI
Primary accession numberP0A7D7
Secondary accession number(s) P21155
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 6)
Name and origin of the protein
DescriptionRecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase; EC=6.3.2.6; AltName: Full=SAICAR synthetase;.
Gene nameName=purC
OrderedLocusNames=b2476, JW2461
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMOTRIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location 		 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.09; Mw=26148  [identification data]

EXPRESSION:
increase after benzoic acid treatment [1].

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEB026.3; 6TH EDITION.
UniProtKB/Swiss-ProtP0A7D7; PUR7_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePUR7_ECOLI
Primary accession numberP0A7D7
Secondary accession number(s) P21155
Sequence was last modified on June 7, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 63)
Name and origin of the protein
DescriptionRecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase; EC=6.3.2.6; AltName: Full=SAICAR synthetase;
Gene nameName=purC
OrderedLocusNames=b2476, JW2461
Encoded onName=purC; OrderedLocusNames=b2476, JW2461
Keywords3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; Nucleotide-binding; Purine biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM33928; AAA24448.1; -; Genomic_DNA
EMBLU00096; AAC75529.1; -; Genomic_DNA
EMBLAP009048; BAA16353.1; -; Genomic_DNA
EMBLX57402; CAA40662.1; -; Genomic_DNA
PIRC36146; C36146; .
RefSeqNP_416971.1; NC_000913.2; .
PDB2GQR; X-ray; 2.00 A; A/B=1-237
PDB2GQS; X-ray; 2.05 A; A/B=1-237
PDBsum2GQR; -; .
PDBsum2GQS; -; .
ProteinModelPortalP0A7D7; -; .
SMRP0A7D7; 1-237; .
DIPDIP-35900N; -; .
IntActP0A7D7; 17; .
MINTMINT-1257522; -; .
SWISS-2DPAGEP0A7D7; -; .
ECO2DBASEB026.3; 6TH EDITION; .
EnsemblBacteriaEBESCT00000003853; EBESCP00000003853; EBESCG00000003148; .
EnsemblBacteriaEBESCT00000014498; EBESCP00000013789; EBESCG00000013559; .
GeneID946957; -; .
GenomeReviewsAP009048_GR; JW2461; .
GenomeReviewsU00096_GR; b2476; .
KEGGecj:JW2461; -; .
KEGGeco:b2476; -; .
EchoBASEEB0784; -; .
EcoGeneEG10791; purC; .
eggNOGCOG0152; -; .
GeneTreeEBGT00050000009817; -; .
HOGENOMHBG306070; -; .
OMAYKDDALG; -; .
ProtClustDBPRK09362; -; .
BioCycEcoCyc:SAICARSYN-MONOMER; -; .
BioCycMetaCyc:SAICARSYN-MONOMER; -; .
GenevestigatorP0A7D7; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IEA:EC; .
GOGO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW; .
HAMAPMF_00137; SAICAR_synth; 1; -
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001636; SAICAR_synth; .
InterProIPR018236; SAICAR_synthetase_CS; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
PANTHERPTHR11609; SAICAR_synt; 1; .
PfamPF01259; SAICAR_synt; 1; .
TIGRFAMsTIGR00081; PurC; 1; .
PROSITEPS01057; SAICAR_SYNTHETASE_1; 1; .
PROSITEPS01058; SAICAR_SYNTHETASE_2; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server