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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P0A7D7 SWISS-2DPAGE:  P0A7D7 P0A7D7 General information about the entry View entry in simple text format Entry name PUR7_ECOLI Primary accession number P0A7D7 Secondary accession number(s) P21155 integrated into SWISS-2DPAGE on  May 15, 2003 (release 16) 2D Annotations were last modified on May 15, 2003 (version 1) General Annotations were last modified on  May 19, 2011 (version 6) Name and origin of the protein Description RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase; EC=6.3.2.6; AltName: Full=SAICAR synthetase;. Gene name Name=purC OrderedLocusNames=b2476, JW2461 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan] Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'' Proteomics 2(1):1682-1698(2002) Comments SUBUNIT: HOMOTRIMER 2D PAGE maps for identified proteins How to interpret a protein ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI-DIGE4.5-6.5 MAP LOCATIONS: SPOT 2D-001WT3: pI=5.09; Mw=26148   [identification data] EXPRESSION: increase after benzoic acid treatment [1]. MAPPING (identification): Peptide mass fingerprinting [1]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references ECO2DBASE B026.3; 6TH EDITION. UniProtKB/Swiss-Prot P0A7D7; PUR7_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name PUR7_ECOLI Primary accession number P0A7D7 Secondary accession number(s) P21155 Sequence was last modified on  June 7, 2005 (version 1) Annotations were last modified on  October 19, 2011 (version 63) Name and origin of the protein Description RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase; EC=6.3.2.6; AltName: Full=SAICAR synthetase; Gene name Name=purC OrderedLocusNames=b2476, JW2461 Encoded on Name=purC; OrderedLocusNames=b2476, JW2461 Keywords 3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; Nucleotide-binding; Purine biosynthesis; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M33928; AAA24448.1; -; Genomic_DNA EMBL U00096; AAC75529.1; -; Genomic_DNA EMBL AP009048; BAA16353.1; -; Genomic_DNA EMBL X57402; CAA40662.1; -; Genomic_DNA PIR C36146; C36146; . RefSeq NP_416971.1; NC_000913.2; . PDB 2GQR; X-ray; 2.00 A; A/B=1-237 PDB 2GQS; X-ray; 2.05 A; A/B=1-237 PDBsum 2GQR; -; . PDBsum 2GQS; -; . ProteinModelPortal P0A7D7; -; . SMR P0A7D7; 1-237; . DIP DIP-35900N; -; . IntAct P0A7D7; 17; . MINT MINT-1257522; -; . SWISS-2DPAGE P0A7D7; -; . ECO2DBASE B026.3; 6TH EDITION; . EnsemblBacteria EBESCT00000003853; EBESCP00000003853; EBESCG00000003148; . EnsemblBacteria EBESCT00000014498; EBESCP00000013789; EBESCG00000013559; . GeneID 946957; -; . GenomeReviews AP009048_GR; JW2461; . GenomeReviews U00096_GR; b2476; . KEGG ecj:JW2461; -; . KEGG eco:b2476; -; . EchoBASE EB0784; -; . EcoGene EG10791; purC; . eggNOG COG0152; -; . GeneTree EBGT00050000009817; -; . HOGENOM HBG306070; -; . OMA YKDDALG; -; . ProtClustDB PRK09362; -; . BioCyc EcoCyc:SAICARSYN-MONOMER; -; . BioCyc MetaCyc:SAICARSYN-MONOMER; -; . Genevestigator P0A7D7; -; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0016020; C:membrane; IDA:UniProtKB; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IEA:EC; . GO GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW; . HAMAP MF_00137; SAICAR_synth; 1; - InterPro IPR013816; ATP_grasp_subdomain_2; . InterPro IPR001636; SAICAR_synth; . InterPro IPR018236; SAICAR_synthetase_CS; . Gene3D G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; . PANTHER PTHR11609; SAICAR_synt; 1; . Pfam PF01259; SAICAR_synt; 1; . TIGRFAMs TIGR00081; PurC; 1; . PROSITE PS01057; SAICAR_SYNTHETASE_1; 1; . PROSITE PS01058; SAICAR_SYNTHETASE_2; 1; . SWISS-2DPAGE (search AC)