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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A7Z4




SWISS-2DPAGE:  P0A7Z4


P0A7Z4


General information about the entry
View entry in simple text format
Entry nameRPOA_ECOLI
Primary accession numberP0A7Z4
Secondary accession number(s) P00574
integrated into SWISS-2DPAGE on October 1, 1998 (release 8)
2D Annotations were last modified onMarch 31, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=DNA-directed RNA polymerase subunit alpha; Short=RNAP subunit alpha; EC=2.7.7.6; AltName: Full=RNA polymerase subunit alpha; AltName: Full=Transcriptase subunit alpha;.
Gene nameName=rpoA
Synonyms=pez, phs, sez
OrderedLocusNames=b3295, JW3257
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.03; Mw=39816  [identification data]
pI=5.06; Mw=39655  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.03; Mw=39691  [identification data]
pI=5.07; Mw=39523  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2];
SPOT 2D-001WMU: TANDEM MASS SPECTROMETRY [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEB040.7; 6TH EDITION.
UniProtKB/Swiss-ProtP0A7Z4; RPOA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRPOA_ECOLI
Primary accession numberP0A7Z4
Secondary accession number(s) P00574 Q2M6W0
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 71)
Name and origin of the protein
DescriptionRecName: Full=DNA-directed RNA polymerase subunit alpha; Short=RNAP subunit alpha; EC=2.7.7.6; AltName: Full=RNA polymerase subunit alpha; AltName: Full=Transcriptase subunit alpha;
Gene nameName=rpoA
Synonyms=pez, phs, sez
OrderedLocusNames=b3295, JW3257
Encoded onName=rpoA; Synonyms=pez, phs, sez; OrderedLocusNames=b3295, JW3257
Keywords3D-structure; Complete proteome; Direct protein sequencing; DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome; Transcription; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01685; AAA24577.1; -; Genomic_DNA
EMBLX00766; CAA25337.1; -; Genomic_DNA
EMBLX02543; CAA26395.1; -; Genomic_DNA
EMBLU18997; AAA58092.1; -; Genomic_DNA
EMBLX53843; CAA37838.1; -; Genomic_DNA
EMBLX53844; CAA37839.1; -; Genomic_DNA
EMBLU00096; AAC76320.1; -; Genomic_DNA
EMBLAP009048; BAE77996.1; -; Genomic_DNA
EMBLV00353; CAA23646.1; -; Genomic_DNA
EMBLM29822; AAA24590.1; -; Genomic_DNA
EMBLM29823; AAA24592.1; -; Genomic_DNA
EMBLM29824; AAA24594.1; -; Genomic_DNA
PIRA22884; RNECA; .
RefSeqNP_417754.1; NC_000913.2; .
PDB1BDF; X-ray; 2.50 A; A/B/C/D=1-235
PDB1COO; NMR; -; A=233-329
PDB1LB2; X-ray; 3.10 A; B/E=246-329
PDB1XS9; NMR; -; D=249-329
PDB3IYD; EM; -; A/B=1-329
PDB3K4G; X-ray; 2.05 A; A/B/C/D/E/F/G/H=245-329
PDB3LU0; EM; -; A/B=1-329
PDB3N4M; X-ray; 2.99 A; B/C=246-329
PDB3N97; X-ray; 3.25 A; B/C=246-329
PDBsum1BDF; -; .
PDBsum1COO; -; .
PDBsum1LB2; -; .
PDBsum1XS9; -; .
PDBsum3IYD; -; .
PDBsum3K4G; -; .
PDBsum3LU0; -; .
PDBsum3N4M; -; .
PDBsum3N97; -; .
ProteinModelPortalP0A7Z4; -; .
SMRP0A7Z4; 1-329; .
DIPDIP-35879N; -; .
IntActP0A7Z4; 83; .
SWISS-2DPAGEP0A7Z4; -; .
ECO2DBASEB040.7; 6TH EDITION; .
PRIDEP0A7Z4; -; .
EnsemblBacteriaEBESCT00000004672; EBESCP00000004672; EBESCG00000003814; .
EnsemblBacteriaEBESCT00000014914; EBESCP00000014205; EBESCG00000013974; .
GeneID947794; -; .
GenomeReviewsAP009048_GR; JW3257; .
GenomeReviewsU00096_GR; b3295; .
KEGGecj:JW3257; -; .
KEGGeco:b3295; -; .
EchoBASEEB0886; -; .
EcoGeneEG10893; rpoA; .
eggNOGCOG0202; -; .
GeneTreeEBGT00050000010928; -; .
HOGENOMHBG430844; -; .
OMAVRSHNCL; -; .
ProtClustDBPRK05182; -; .
BioCycEcoCyc:EG10893-MONOMER; -; .
BioCycMetaCyc:EG10893-MONOMER; -; .
DrugBankDB00615; Rifabutin; .
GenevestigatorP0A7Z4; -; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0003677; F:DNA binding; IEA:InterPro; .
GOGO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW; .
GOGO:0046983; F:protein dimerization activity; IEA:InterPro; .
GOGO:0008270; F:zinc ion binding; IDA:EcoliWiki; .
HAMAPMF_00059; RNApol_bact_RpoA; 1; -
InterProIPR011261; DNA-dir_RNA_pol_dimersation; .
InterProIPR011262; DNA-dir_RNA_pol_insert; .
InterProIPR009025; DNA-dir_RNA_pol_RBP11-like; .
InterProIPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3; .
InterProIPR011773; DNA-dir_RpoA; .
InterProIPR011260; RNAP_asu_C; .
Gene3DG3DSA:2.170.120.12; RNAP_insert; 1; .
PfamPF01000; RNA_pol_A_bac; 1; .
PfamPF03118; RNA_pol_A_CTD; 1; .
PfamPF01193; RNA_pol_L; 1; .
ProDomPD001179; RNAP_asu_C; 1; .
SMARTSM00662; RPOLD; 1; .
SUPFAMSSF47789; RNAP_alpha_C; 1; .
SUPFAMSSF56553; RNAP_insert; 1; .
SUPFAMSSF55257; RNAP_RBP11-like; 1; .
TIGRFAMsTIGR02027; RpoA; 1; .



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