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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AAI9




SWISS-2DPAGE:  P0AAI9


P0AAI9


General information about the entry
View entry in simple text format
Entry nameFABD_ECOLI
Primary accession numberP0AAI9
Secondary accession number(s) P25715
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Malonyl CoA-acyl carrier protein transacylase; Short=MCT; EC=2.3.1.39;.
Gene nameName=fabD
Synonyms=tfpA
OrderedLocusNames=b1092, JW1078
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[4]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.37; Mw=44970
pI=4.95; Mw=33469

MAPPING (identification):
GEL MATCHING OR MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.03; Mw=32271  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.03; Mw=33449  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP0AAI9; FABD_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameFABD_ECOLI
Primary accession numberP0AAI9
Secondary accession number(s) P25715
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 56)
Name and origin of the protein
DescriptionRecName: Full=Malonyl CoA-acyl carrier protein transacylase; Short=MCT; EC=2.3.1.39;
Gene nameName=fabD
Synonyms=tfpA
OrderedLocusNames=b1092, JW1078
Encoded onName=fabD; Synonyms=tfpA; OrderedLocusNames=b1092, JW1078
Keywords3D-structure; Acyltransferase; Complete proteome; Direct protein sequencing; Fatty acid biosynthesis; Lipid synthesis; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM87040; AAA23742.1; -; Genomic_DNA
EMBLZ11565; CAA77658.1; -; Genomic_DNA
EMBLU00096; AAC74176.1; -; Genomic_DNA
EMBLAP009048; BAA35900.1; -; Genomic_DNA
EMBLM84991; AAA23738.1; -; Genomic_DNA
PIRB41856; B41856; .
RefSeqNP_415610.1; NC_000913.2; .
PDB1MLA; X-ray; 1.50 A; A=1-309
PDB2G1H; X-ray; 1.86 A; A=2-308
PDB2G2O; X-ray; 1.76 A; A=2-308
PDB2G2Y; X-ray; 2.26 A; A=2-308
PDB2G2Z; X-ray; 2.80 A; A=2-308
PDBsum1MLA; -; .
PDBsum2G1H; -; .
PDBsum2G2O; -; .
PDBsum2G2Y; -; .
PDBsum2G2Z; -; .
ProteinModelPortalP0AAI9; -; .
SMRP0AAI9; 3-307; .
DIPDIP-47923N; -; .
IntActP0AAI9; 3; .
SWISS-2DPAGEP0AAI9; -; .
EnsemblBacteriaEBESCT00000005075; EBESCP00000005075; EBESCG00000004140; .
EnsemblBacteriaEBESCT00000016146; EBESCP00000015437; EBESCG00000015206; .
GeneID945766; -; .
GenomeReviewsAP009048_GR; JW1078; .
GenomeReviewsU00096_GR; b1092; .
KEGGecj:JW1078; -; .
KEGGeco:b1092; -; .
EchoBASEEB1293; -; .
EcoGeneEG11317; fabD; .
eggNOGCOG0331; -; .
GeneTreeEBGT00050000008801; -; .
HOGENOMHBG687898; -; .
OMANAMQAAV; -; .
ProtClustDBCLSK879951; -; .
BioCycEcoCyc:MALONYL-COA-ACP-TRANSACYL-MONOMER; -; .
BioCycMetaCyc:MALONYL-COA-ACP-TRANSACYL-MONOMER; -; .
GenevestigatorP0AAI9; -; .
GOGO:0004314; F:[acyl-carrier-protein] S-malonyltransferase activity; IEA:EC; .
GOGO:0005488; F:binding; IEA:InterPro; .
GOGO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW; .
InterProIPR001227; Ac_transferase_dom; .
InterProIPR014043; Acyl_transferase; .
InterProIPR016035; Acyl_Trfase/lysoPLipase; .
InterProIPR004410; FabD; .
InterProIPR016036; Malonyl_transacylase_ACP-bd; .
Gene3DG3DSA:3.40.366.10; Ac_transferase_reg; 2; .
PfamPF00698; Acyl_transf_1; 1; .
SUPFAMSSF52151; Acyl_Trfase/lysoPlipase; 1; .
SUPFAMSSF55048; Malonyl_transacylase_ACP-bd; 1; .
TIGRFAMsTIGR00128; FabD; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server