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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0ABA0




SWISS-2DPAGE:  P0ABA0


P0ABA0


General information about the entry
View entry in simple text format
Entry nameATPF_ECOLI
Primary accession numberP0ABA0
Secondary accession number(s) P00859
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=ATP synthase subunit b; AltName: Full=ATP synthase F(0) sector subunit b; AltName: Full=ATPase subunit I; AltName: Full=F-type ATPase subunit b; Short=F-ATPase subunit b;.
Gene nameName=atpF
Synonyms=papF, uncF
OrderedLocusNames=b3736, JW3714
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: F-TYPE ATPASES HAVE 2 COMPONENTS, CF(1) - THE CATALYTIC CORE - AND CF(0) - THE MEMBRANE PROTON CHANNEL. CF(1) HAS FIVE SUBUNITS: ALPHA(3), BETA(3), GAMMA(1), DELTA(1), EPSILON(1). CF(0) HAS THREE MAIN SUBUNITS: A, B AND C
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.53; Mw=19289  [identification data]
pI=5.53; Mw=18722  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [1].

MAPPING (identification):
Peptide mass fingerprinting [1];
SPOT 2D-001WWO: TANDEM MASS SPECTROMETRY [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP0ABA0; ATPF_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameATPF_ECOLI
Primary accession numberP0ABA0
Secondary accession number(s) P00859 Q2M854
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 60)
Name and origin of the protein
DescriptionRecName: Full=ATP synthase subunit b; AltName: Full=ATP synthase F(0) sector subunit b; AltName: Full=ATPase subunit I; AltName: Full=F-type ATPase subunit b; Short=F-ATPase subunit b;
Gene nameName=atpF
Synonyms=papF, uncF
OrderedLocusNames=b3736, JW3714
Encoded onName=atpF; Synonyms=papF, uncF; OrderedLocusNames=b3736, JW3714
Keywords3D-structure; ATP synthesis; Cell inner membrane; Cell membrane; CF(0); Complete proteome; Hydrogen ion transport; Ion transport; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01594; AAA24733.1; -; Genomic_DNA
EMBLX01631; CAA25778.1; -; Genomic_DNA
EMBLV00264; CAA23516.1; -; Genomic_DNA
EMBLV00310; CAA23592.1; -; Genomic_DNA
EMBLV00266; CAA23523.1; -; Genomic_DNA
EMBLM25464; AAA83871.1; -; Genomic_DNA
EMBLM10422; AAA24741.1; -; Genomic_DNA
EMBLL10328; AAA62088.1; -; Genomic_DNA
EMBLU00096; AAC76759.1; -; Genomic_DNA
EMBLAP009048; BAE77552.1; -; Genomic_DNA
EMBLM12212; AAA20043.1; -; Unassigned_DNA
PIRD93732; LWECB; .
RefSeqNP_418192.1; NC_000913.2; .
PDB1B9U; NMR; -; A=1-34
PDB1L2P; X-ray; 1.55 A; A=62-122
PDB2KHK; NMR; -; A=30-82
PDBsum1B9U; -; .
PDBsum1L2P; -; .
PDBsum2KHK; -; .
ProteinModelPortalP0ABA0; -; .
SMRP0ABA0; 1-122; .
DIPDIP-35994N; -; .
IntActP0ABA0; 2; .
TCDB3.A.2.1.1; H+- or Na+-translocating F-type; V-type and A-type ATPase (F-ATPase) superfamily; .
SWISS-2DPAGEP0ABA0; -; .
EnsemblBacteriaEBESCT00000005030; EBESCP00000005030; EBESCG00000004106; .
EnsemblBacteriaEBESCT00000005031; EBESCP00000005031; EBESCG00000004106; .
EnsemblBacteriaEBESCT00000005032; EBESCP00000005032; EBESCG00000004106; .
EnsemblBacteriaEBESCT00000017978; EBESCP00000017269; EBESCG00000017034; .
GeneID948247; -; .
GenomeReviewsAP009048_GR; JW3714; .
GenomeReviewsU00096_GR; b3736; .
KEGGecj:JW3714; -; .
KEGGeco:b3736; -; .
EchoBASEEB0101; -; .
EcoGeneEG10103; atpF; .
eggNOGCOG0711; -; .
GeneTreeEBGT00050000011545; -; .
HOGENOMHBG617328; -; .
OMALEKYNAQ; -; .
ProtClustDBPRK05759; -; .
BioCycEcoCyc:ATPF-MONOMER; -; .
BioCycMetaCyc:ATPF-MONOMER; -; .
GenevestigatorP0ABA0; -; .
GOGO:0031225; C:anchored to membrane; IDA:EcoliWiki; .
GOGO:0016021; C:integral to membrane; IEA:UniProtKB-KW; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0045263; C:proton-transporting ATP synthase complex; coupling factor F(o); IMP:EcoliWiki
GOGO:0015078; F:hydrogen ion transmembrane transporter activity; IEA:InterPro; .
GOGO:0015986; P:ATP synthesis coupled proton transport; IMP:EcoliWiki; .
HAMAPMF_01398; ATP_synth_b_bact; 1; -
InterProIPR002146; ATPase_F0-cplx_b/b'su_bac; .
InterProIPR005864; ATPase_F0-cplx_bsu_bac; .
PfamPF00430; ATP-synt_B; 1; .
TIGRFAMsTIGR01144; ATP_synt_b; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server