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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AEE5




SWISS-2DPAGE:  P0AEE5


P0AEE5


General information about the entry
View entry in simple text format
Entry nameDGAL_ECOLI
Primary accession numberP0AEE5
Secondary accession number(s) P02927
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=D-galactose-binding periplasmic protein; Short=GBP; AltName: Full=D-galactose/ D-glucose-binding protein; Short=GGBP; Flags: Precursor;.
Gene nameName=mglB
OrderedLocusNames=b2150, JW2137
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.16; Mw=31379

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.19; Mw=31245  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0AEE5; DGAL_ECOLI.
ECO2DBASEC029.8; 6TH EDITION.
UniProtKB/Swiss-ProtP0AEE5; DGAL_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDGAL_ECOLI
Primary accession numberP0AEE5
Secondary accession number(s) P02927 P17775 Q2MAS9
Sequence was last modified on August 13, 1987 (version 1)
Annotations were last modified on October 19, 2011 (version 53)
Name and origin of the protein
DescriptionRecName: Full=D-galactose-binding periplasmic protein; Short=GBP; AltName: Full=D-galactose/ D-glucose-binding protein; Short=GGBP; Flags: Precursor;
Gene nameName=mglB
OrderedLocusNames=b2150, JW2137
Encoded onName=mglB; OrderedLocusNames=b2150, JW2137
Keywords3D-structure; Calcium; Chemotaxis; Complete proteome; Direct protein sequencing; Periplasm; Reference proteome; Signal; Sugar transport; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM59444; AAA24169.1; -; Genomic_DNA
EMBLU00007; AAA60523.1; -; Genomic_DNA
EMBLX05646; CAA29132.1; -; Genomic_DNA
EMBLU00096; AAC75211.1; -; Genomic_DNA
EMBLAP009048; BAE76627.1; -; Genomic_DNA
EMBLK00419; AAA24172.1; -; Genomic_DNA
PIRA37277; JGECG; .
RefSeqNP_416655.1; NC_000913.2; .
PDB1GLG; X-ray; 2.00 A; A=24-332
PDB2FVY; X-ray; 0.92 A; A=24-332
PDB2FW0; X-ray; 1.55 A; A=24-332
PDB2GBP; X-ray; 1.90 A; A=24-332
PDB2HPH; X-ray; 1.33 A; A=24-332
PDB2IPL; X-ray; 1.20 A; A=24-332
PDB2IPM; X-ray; 1.12 A; A=24-332
PDB2IPN; X-ray; 1.15 A; A=24-332
PDB2QW1; X-ray; 1.70 A; A=24-332
PDBsum1GLG; -; .
PDBsum2FVY; -; .
PDBsum2FW0; -; .
PDBsum2GBP; -; .
PDBsum2HPH; -; .
PDBsum2IPL; -; .
PDBsum2IPM; -; .
PDBsum2IPN; -; .
PDBsum2QW1; -; .
ProteinModelPortalP0AEE5; -; .
SMRP0AEE5; 25-329; .
DIPDIP-35953N; -; .
IntActP0AEE5; 7; .
MINTMINT-1274707; -; .
TCDB3.A.1.2.3; ATP-binding cassette (ABC) superfamily; .
SWISS-2DPAGEP0AEE5; -; .
2DBase-EcoliP0AEE5; -; .
ECO2DBASEC029.8; 6TH EDITION; .
PRIDEP0AEE5; -; .
EnsemblBacteriaEBESCT00000004344; EBESCP00000004344; EBESCG00000003540; .
EnsemblBacteriaEBESCT00000014412; EBESCP00000013703; EBESCG00000013473; .
GeneID949041; -; .
GenomeReviewsAP009048_GR; JW2137; .
GenomeReviewsU00096_GR; b2150; .
KEGGecj:JW2137; -; .
KEGGeco:b2150; -; .
EchoBASEEB0588; -; .
EcoGeneEG10593; mglB; .
eggNOGCOG1879; -; .
GeneTreeEBGT00050000009242; -; .
HOGENOMHBG617058; -; .
OMAKIENKVV; -; .
ProtClustDBPRK15395; -; .
BioCycEcoCyc:MGLB-MONOMER; -; .
GenevestigatorP0AEE5; -; .
GOGO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell; .
GOGO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW; .
GOGO:0006935; P:chemotaxis; IEA:UniProtKB-KW; .
InterProIPR001761; Peripla_BP_Lac1; .
PfamPF00532; Peripla_BP_1; 1; .



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