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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AEK4




SWISS-2DPAGE:  P0AEK4


P0AEK4


General information about the entry
View entry in simple text format
Entry nameFABI_ECOLI
Primary accession numberP0AEK4
Secondary accession number(s) P29132
integrated into SWISS-2DPAGE on April 1, 2000 (release 12)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] FabI; Short=ENR; EC=1.3.1.9; AltName: Full=NADH-dependent enoyl-ACP reductase;.
Gene nameName=fabI
Synonyms=envM
OrderedLocusNames=b1288, JW1281
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.37; Mw=38366  [identification data]
pI=5.33; Mw=37266  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.40; Mw=32959  [identification data]
pI=5.60; Mw=32613  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [2].

MAPPING (identification):
Tandem mass spectrometry [2];
SPOT 2D-001WQS: PEPTIDE MASS FINGERPRINTING [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP0AEK4; FABI_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameFABI_ECOLI
Primary accession numberP0AEK4
Secondary accession number(s) P29132
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 64)
Name and origin of the protein
DescriptionRecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] FabI; Short=ENR; EC=1.3.1.9; AltName: Full=NADH-dependent enoyl-ACP reductase;
Gene nameName=fabI
Synonyms=envM
OrderedLocusNames=b1288, JW1281
Encoded onName=fabI; Synonyms=envM; OrderedLocusNames=b1288, JW1281
Keywords3D-structure; Antibiotic resistance; Complete proteome; Direct protein sequencing; Fatty acid biosynthesis; Lipid synthesis; NAD; Oxidoreductase; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM97219; AAA17755.1; -; Unassigned_DNA
EMBLX78733; CAA55381.1; -; Genomic_DNA
EMBLU00096; AAC74370.1; -; Genomic_DNA
EMBLAP009048; BAA14841.1; -; Genomic_DNA
PIRS48029; S48029; .
RefSeqNP_415804.1; NC_000913.2; .
PDB1C14; X-ray; 2.00 A; A/B=1-262
PDB1D8A; X-ray; 2.20 A; A/B=2-262
PDB1DFG; X-ray; 2.50 A; A/B=2-262
PDB1DFH; X-ray; 2.20 A; A/B=2-262
PDB1DFI; X-ray; 2.09 A; A/B/C/D=2-262
PDB1I2Z; X-ray; 2.80 A; A/B=1-262
PDB1I30; X-ray; 2.40 A; A/B=1-262
PDB1LX6; X-ray; 2.40 A; A/B=1-262
PDB1LXC; X-ray; 2.40 A; A/B=1-262
PDB1MFP; X-ray; 2.33 A; A/B=1-262
PDB1QG6; X-ray; 1.90 A; A/B/C/D=2-262
PDB1QSG; X-ray; 1.75 A; A/B/C/D/E/F/G/H=1-262
PDB2FHS; X-ray; 2.70 A; A/B=1-262
PDB3PJD; X-ray; 2.50 A; A/B=1-262
PDB3PJE; X-ray; 2.50 A; A/B=1-262
PDB3PJF; X-ray; 1.90 A; A/B=1-262
PDBsum1C14; -; .
PDBsum1D8A; -; .
PDBsum1DFG; -; .
PDBsum1DFH; -; .
PDBsum1DFI; -; .
PDBsum1I2Z; -; .
PDBsum1I30; -; .
PDBsum1LX6; -; .
PDBsum1LXC; -; .
PDBsum1MFP; -; .
PDBsum1QG6; -; .
PDBsum1QSG; -; .
PDBsum2FHS; -; .
PDBsum3PJD; -; .
PDBsum3PJE; -; .
PDBsum3PJF; -; .
ProteinModelPortalP0AEK4; -; .
SMRP0AEK4; 2-260; .
DIPDIP-31867N; -; .
IntActP0AEK4; 12; .
MINTMINT-1251203; -; .
SWISS-2DPAGEP0AEK4; -; .
EnsemblBacteriaEBESCT00000000533; EBESCP00000000533; EBESCG00000000449; .
EnsemblBacteriaEBESCT00000014396; EBESCP00000013687; EBESCG00000013457; .
GeneID945870; -; .
GenomeReviewsAP009048_GR; JW1281; .
GenomeReviewsU00096_GR; b1288; .
KEGGecj:JW1281; -; .
KEGGeco:b1288; -; .
EchoBASEEB1490; -; .
EcoGeneEG11528; fabI; .
eggNOGCOG0623; -; .
GeneTreeEBGT00050000009187; -; .
HOGENOMHBG750976; -; .
OMADCDVGSD; -; .
ProtClustDBPRK07984; -; .
BioCycEcoCyc:ENOYL-ACP-REDUCT-NADH-MONOMER; -; .
BioCycMetaCyc:ENOYL-ACP-REDUCT-NADH-MONOMER; -; .
BindingDBP0AEK4; -; .
GenevestigatorP0AEK4; -; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0008667; F:2; 3-dihydro-2; 3-dihydroxybenzoate dehydrogenase activity
IDA:EcoCyc.
GOGO:0005488; F:binding; IEA:InterPro; .
GOGO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IDA:UniProtKB; .
GOGO:0009239; P:enterobactin biosynthetic process; IMP:EcoCyc; .
GOGO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0051289; P:protein homotetramerization; IDA:UniProtKB; .
GOGO:0046677; P:response to antibiotic; IEA:UniProtKB-KW; .
InterProIPR002198; DH_sc/Rdtase_SDR; .
InterProIPR014358; Enoyl-ACP_Rdtase_NADH; .
InterProIPR002347; Glc/ribitol_DH; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PfamPF00106; adh_short; 1; .
PIRSFPIRSF000094; Enoyl-ACP_rdct; 1; .
PRINTSPR00081; GDHRDH; .



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