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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AES4




SWISS-2DPAGE:  P0AES4


P0AES4


General information about the entry
View entry in simple text format
Entry nameGYRA_ECOLI
Primary accession numberP0AES4
Secondary accession number(s) P09097
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=DNA gyrase subunit A; EC=5.99.1.3;.
Gene nameName=gyrA
Synonyms=hisW, nalA, parD
OrderedLocusNames=b2231, JW2225
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
Comments
  • SUBUNIT: HETERODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.27; Mw=100170

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
ECO2DBASED101.5; 6TH EDITION.
UniProtKB/Swiss-ProtP0AES4; GYRA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGYRA_ECOLI
Primary accession numberP0AES4
Secondary accession number(s) P09097
Sequence was last modified on December 20, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 61)
Name and origin of the protein
DescriptionRecName: Full=DNA gyrase subunit A; EC=5.99.1.3;
Gene nameName=gyrA
Synonyms=hisW, nalA, parD
OrderedLocusNames=b2231, JW2225
Encoded onName=gyrA; Synonyms=hisW, nalA, parD; OrderedLocusNames=b2231, JW2225
Keywords3D-structure; Antibiotic resistance; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Isomerase; Nucleotide-binding; Reference proteome; Topoisomerase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX06373; CAA29676.1; -; Genomic_DNA
EMBLX06744; CAA29919.1; -; Genomic_DNA
EMBLM15631; AAA23948.1; -; Genomic_DNA
EMBLU00096; AAC75291.1; -; Genomic_DNA
EMBLAP009048; BAA16048.1; -; Genomic_DNA
EMBLY00544; CAA68611.1; -; Genomic_DNA
PIRS02340; ITECAP; .
RefSeqNP_416734.1; NC_000913.2; .
PDB1AB4; X-ray; 2.80 A; A=30-522
PDB1X75; X-ray; 2.80 A; A/B=363-494
PDB1ZI0; X-ray; 2.60 A; A/B=535-841
PDB2Y3P; X-ray; 2.62 A; A/B=2-523
PDB3KU8; X-ray; 1.93 A; A/B=363-497
PDB3NUH; X-ray; 3.10 A; A=1-525
PDBsum1AB4; -; .
PDBsum1X75; -; .
PDBsum1ZI0; -; .
PDBsum2Y3P; -; .
PDBsum3KU8; -; .
PDBsum3NUH; -; .
ProteinModelPortalP0AES4; -; .
SMRP0AES4; 30-522; 535-841; .
DIPDIP-36179N; -; .
IntActP0AES4; 52; .
MINTMINT-201682; -; .
SWISS-2DPAGEP0AES4; -; .
ECO2DBASED101.5; 6TH EDITION; .
PRIDEP0AES4; -; .
EnsemblBacteriaEBESCT00000004656; EBESCP00000004656; EBESCG00000003798; .
EnsemblBacteriaEBESCT00000017137; EBESCP00000016428; EBESCG00000016196; .
GeneID946614; -; .
GenomeReviewsAP009048_GR; JW2225; .
GenomeReviewsU00096_GR; b2231; .
KEGGecj:JW2225; -; .
KEGGeco:b2231; -; .
EchoBASEEB0418; -; .
EcoGeneEG10423; gyrA; .
eggNOGCOG0188; -; .
GeneTreeEBGT00050000010885; -; .
HOGENOMHBG635183; -; .
OMATGRGRIY; -; .
ProtClustDBPRK05560; -; .
BioCycEcoCyc:EG10423-MONOMER; -; .
BioCycMetaCyc:EG10423-MONOMER; -; .
GenevestigatorP0AES4; -; .
GOGO:0005694; C:chromosome; IEA:InterPro; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0003918; F:DNA topoisomerase (ATP-hydrolyzing) activity; IEA:EC; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0006265; P:DNA topological change; IEA:InterPro; .
GOGO:0006355; P:regulation of transcription; DNA-dependent; IEA:InterPro
GOGO:0046677; P:response to antibiotic; IEA:UniProtKB-KW; .
HAMAPMF_01897; GyrA; 1; -
InterProIPR020899; Arg_repress_C; .
InterProIPR005743; GyrA; .
InterProIPR006691; GyrA/parC_pinwhl; .
InterProIPR002205; Topo_IIA_A/C; .
InterProIPR013758; Topo_IIA_A/C_ab; .
InterProIPR013757; Topo_IIA_A_a; .
InterProIPR013760; Topo_IIA_cen; .
Gene3DG3DSA:3.30.1360.40; Arg_repress; 1; .
Gene3DG3DSA:3.90.199.10; Topo_IIA_A/C_ab; 1; .
Gene3DG3DSA:1.10.268.10; Topo_IIA_A_a; 1; .
PfamPF03989; DNA_gyraseA_C; 6; .
PfamPF00521; DNA_topoisoIV; 1; .
SMARTSM00434; TOP4c; 1; .
SUPFAMSSF56719; Topo_IIA_cen; 1; .
TIGRFAMsTIGR01063; GyrA; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server