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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AGJ9




SWISS-2DPAGE:  P0AGJ9


P0AGJ9


General information about the entry
View entry in simple text format
Entry nameSYY_ECOLI
Primary accession numberP0AGJ9
Secondary accession number(s) P00951
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onNovember 9, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Tyrosyl-tRNA synthetase; EC=6.1.1.1; AltName: Full=Tyrosine--tRNA ligase; Short=TyrRS;.
Gene nameName=tyrS
OrderedLocusNames=b1637, JW1629
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=81069671; PubMed=7002901; [NCBI, ExPASy, EBI, Israel, Japan]
Phillips T.A., Bloch P.L., Neidhardt F.C.
''''''Protein identifications on O'Farrell two-dimensional gels: locations of 55 additional Escherichia coli proteins'';'';''
J. Bacteriol. 144(1):1024-1033(1980)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.59; Mw=44615

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND PHILLIPS [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEG044.0; 6TH EDITION.
UniProtKB/Swiss-ProtP0AGJ9; SYY_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameSYY_ECOLI
Primary accession numberP0AGJ9
Secondary accession number(s) P00951
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 62)
Name and origin of the protein
DescriptionRecName: Full=Tyrosyl-tRNA synthetase; EC=6.1.1.1; AltName: Full=Tyrosine--tRNA ligase; Short=TyrRS;
Gene nameName=tyrS
OrderedLocusNames=b1637, JW1629
Encoded onName=tyrS; OrderedLocusNames=b1637, JW1629
Keywords3D-structure; Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01719; AAA24707.1; -; Genomic_DNA
EMBLU00096; AAC74709.1; -; Genomic_DNA
EMBLAP009048; BAA15398.1; -; Genomic_DNA
EMBLM92351; AAA24710.1; -; Genomic_DNA
PIRA01178; SYECYT; .
RefSeqNP_416154.1; NC_000913.2; .
PDB1VBM; X-ray; 2.70 A; A/B=5-322
PDB1VBN; X-ray; 2.70 A; A/B=5-322
PDB1WQ3; X-ray; 2.00 A; A=1-322
PDB1WQ4; X-ray; 2.00 A; A=2-322
PDB1X8X; X-ray; 2.00 A; A=1-322
PDB2YXN; X-ray; 1.80 A; A=1-322
PDBsum1VBM; -; .
PDBsum1VBN; -; .
PDBsum1WQ3; -; .
PDBsum1WQ4; -; .
PDBsum1X8X; -; .
PDBsum2YXN; -; .
ProteinModelPortalP0AGJ9; -; .
SMRP0AGJ9; 1-423; .
DIPDIP-36227N; -; .
IntActP0AGJ9; 8; .
MINTMINT-1224558; -; .
SWISS-2DPAGEP0AGJ9; -; .
ECO2DBASEG044.0; 6TH EDITION; .
EnsemblBacteriaEBESCT00000001106; EBESCP00000001106; EBESCG00000000919; .
EnsemblBacteriaEBESCT00000001107; EBESCP00000001107; EBESCG00000000919; .
EnsemblBacteriaEBESCT00000016356; EBESCP00000015647; EBESCG00000015416; .
GeneID948855; -; .
GenomeReviewsAP009048_GR; JW1629; .
GenomeReviewsU00096_GR; b1637; .
KEGGecj:JW1629; -; .
KEGGeco:b1637; -; .
EchoBASEEB1036; -; .
EcoGeneEG11043; tyrS; .
eggNOGCOG0162; -; .
GeneTreeEBGT00050000010726; -; .
HOGENOMHBG288125; -; .
OMATFYIGFD; -; .
ProtClustDBPRK05912; -; .
BioCycEcoCyc:TYRS-MONOMER; -; .
BioCycMetaCyc:TYRS-MONOMER; -; .
GenevestigatorP0AGJ9; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0003723; F:RNA binding; IEA:UniProtKB-KW; .
GOGO:0004831; F:tyrosine-tRNA ligase activity; IEA:EC; .
GOGO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:InterPro; .
HAMAPMF_02006; Tyr_tRNA_synth_type1; 1; -
InterProIPR001412; aa-tRNA-synth_I_CS; .
InterProIPR002305; aa-tRNA-synth_Ic; .
InterProIPR014729; Rossmann-like_a/b/a_fold; .
InterProIPR002942; S4_RNA-bd; .
InterProIPR002307; Tyr-tRNA-synth; .
InterProIPR024088; Tyr-tRNA-synth_bac-type; .
InterProIPR024107; Tyr-tRNA-synth_bac_1; .
Gene3DG3DSA:3.10.290.10; G3DSA:3.10.290.10; 1; .
Gene3DG3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1; .
PANTHERPTHR11766; Tyr_tRNA-synt_1b; 1; .
PfamPF01479; S4; 1; .
PfamPF00579; tRNA-synt_1b; 1; .
PRINTSPR01040; TRNASYNTHTYR; .
SMARTSM00363; S4; 1; .
TIGRFAMsTIGR00234; TyrS; 1; .
PROSITEPS00178; AA_TRNA_LIGASE_I; 1; .
PROSITEPS50889; S4; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server