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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P10649




SWISS-2DPAGE:  P10649


P10649


General information about the entry
View entry in simple text format
Entry nameGSTM1_MOUSE
Primary accession numberP10649
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onMay 15, 2003 (version 4)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Glutathione S-transferase Mu 1; EC=2.5.1.18; AltName: Full=GST 1-1; AltName: Full=GST class-mu 1; AltName: Full=Glutathione S-transferase GT8.7; AltName: Full=pmGT10;.
Gene nameName=Gstm1
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
[2]   MAPPING ON GEL
PubMed=11503206; [NCBI, ExPASy, EBI, Israel, Japan]
Lanne B., Potthast F., Hoglund A., Brockenhuus Von Lowenhielm H., Nystrom A.-C., Nilsson F., Dahllof B.
''''''Thiourea enhances mapping of the proteome from murine white adipose tissue'';'';''
Proteomics 1(1):819-828(2001)
[3]   MAPPING ON GEL
Tonella L., Hoogland C., Sanchez J.-C.
Submitted (OCT-2001) to SWISS-2DPAGE
2D PAGE maps for identified proteins
How to interpret a protein

ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=7.55; Mw=26441  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=8.07; Mw=27086  [identification data]
pI=8.38; Mw=27086
pI=8.22; Mw=27086
pI=7.37; Mw=26861  [identification data]
pI=7.26; Mw=26307  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=8.05; Mw=26655  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=7.95; Mw=29473  [identification data]
pI=8.13; Mw=29605
pI=6.70; Mw=26125
pI=6.20; Mw=13221  [identification data]

MAPPING (identification):
SPOTS 1BZY, 1C0A: MATCHING WITH THE MOUSE LIVER MASTER GEL [1]; SPOTS 1BZY, 1CEW: Peptide mass fingerprinting [1];
SPOT 2D-001C2Z: GEL MATCHING [3] WITH WAT_MOUSE 2-DE MAP FROM LANNE ET AL. [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEP10649; P10649.
UniProtKB/Swiss-ProtP10649; GSTM1_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGSTM1_MOUSE
Primary accession numberP10649
Secondary accession number(s) A2AE90 Q58ET5
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on September 21, 2011 (version 122)
Name and origin of the protein
DescriptionRecName: Full=Glutathione S-transferase Mu 1; EC=2.5.1.18; AltName: Full=GST 1-1; AltName: Full=GST class-mu 1; AltName: Full=Glutathione S-transferase GT8.7; AltName: Full=pmGT10;
Gene nameName=Gstm1
Encoded onName=Gstm1
KeywordsComplete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ03952; AAA37747.1; -; mRNA
EMBLJ04632; AAA37705.1; -; mRNA
EMBLL13448; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLAL671877; CAM17697.1; -; Genomic_DNA
EMBLAC079042; CAM17697.1; JOINED; Genomic_DNA
EMBLBC003822; AAH03822.1; -; mRNA
EMBLBC046758; AAH46758.1; -; mRNA
EMBLBC091763; AAH91763.1; -; mRNA
IPIIPI00230212; -; .
PIRH24735; H24735; .
PIRS30373; S30373; .
PIRS33860; S33860; .
RefSeqNP_034488.1; NM_010358.5; .
UniGeneMm.37199; -; .
ProteinModelPortalP10649; -; .
SMRP10649; 2-218; .
IntActP10649; 1; .
STRINGP10649; -; .
PhosphoSiteP10649; -; .
SWISS-2DPAGEP10649; -; .
REPRODUCTION-2DPAGEP10649; -; .
PRIDEP10649; -; .
EnsemblENSMUST00000004140; ENSMUSP00000004140; ENSMUSG00000058135; .
GeneID14862; -; .
KEGGmmu:14862; -; .
NMPDRfig|10090.3.peg.8801; -; .
UCSCuc008qxx.2; mouse; .
CTD2944; -; .
MGIMGI:95860; Gstm1; .
GeneTreeENSGT00550000074559; -; .
HOVERGENHBG106842; -; .
OrthoDBEOG4RXZ24; -; .
PhylomeDBP10649; -; .
NextBio287105; -; .
ArrayExpressP10649; -; .
BgeeP10649; -; .
CleanExMM_GSTM1; -; .
GenevestigatorP10649; -; .
GermOnlineENSMUSG00000058135; Mus musculus; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0004364; F:glutathione transferase activity; IEA:EC; .
InterProIPR010987; Glutathione-S-Trfase_C-like; .
InterProIPR004045; Glutathione_S-Trfase_N; .
InterProIPR017933; Glutathione_S_Trfase/Cl_chnl_C; .
InterProIPR004046; GST_C; .
InterProIPR003081; GST_mu; .
InterProIPR012336; Thioredoxin-like_fold; .
Gene3DG3DSA:1.20.1050.10; GST_C_like; 1; .
Gene3DG3DSA:3.40.30.10; Thioredoxin_fold; 1; .
PfamPF00043; GST_C; 1; .
PfamPF02798; GST_N; 1; .
PRINTSPR01267; GSTRNSFRASEM; .
SUPFAMSSF47616; GST_C_like; 1; .
SUPFAMSSF52833; Thiordxn-like_fd; 1; .
PROSITEPS50405; GST_CTER; 1; .
PROSITEPS50404; GST_NTER; 1; .



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