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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P16474




SWISS-2DPAGE:  P16474


P16474


General information about the entry
View entry in simple text format
Entry nameGRP78_YEAST
Primary accession numberP16474
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=78 kDa glucose-regulated protein homolog; Short=GRP-78; AltName: Full=Immunoglobulin heavy chain-binding protein homolog; Short=BiP; Flags: Precursor;.
Gene nameName=KAR2
Synonyms=GRP78, SSD1
OrderedLocusNames=YJL034W
ORFNames=J1248
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=91171929; PubMed=2005818; [NCBI, ExPASy, EBI, Israel, Japan]
Nicolet C.M., Craig E.A.
''''''Inducing and assaying heat-shock response in Saccharomyces cerevisiae'';'';''
Meth. Enzymol. 194(1):710-717(1991)
Comments
  • SUBUNIT: MONOMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=4.76; Mw=78975

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2D GELS BY NICOLET AND CRAIG [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP16474; GRP78_YEAST.
YEPD9836.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGRP78_YEAST
Primary accession numberP16474
Secondary accession number(s) D6VWE9
Sequence was last modified on August 1, 1990 (version 1)
Annotations were last modified on October 19, 2011 (version 125)
Name and origin of the protein
DescriptionRecName: Full=78 kDa glucose-regulated protein homolog; Short=GRP-78; AltName: Full=Immunoglobulin heavy chain-binding protein homolog; Short=BiP; Flags: Precursor;
Gene nameName=KAR2
Synonyms=GRP78, SSD1
OrderedLocusNames=YJL034W
ORFNames=J1248
Encoded onName=KAR2; Synonyms=GRP78, SSD1; OrderedLocusNames=YJL034W; ORFNames=J1248
Keywords3D-structure; ATP-binding; Complete proteome; Endoplasmic reticulum; Nucleotide-binding; Phosphoprotein; Reference proteome; Signal; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM25064; AAA34714.1; -; Genomic_DNA
EMBLM25394; AAA34713.1; -; Genomic_DNA
EMBLM31006; AAA34454.1; -; Genomic_DNA
EMBLZ49309; CAA89325.1; -; Genomic_DNA
EMBLBK006943; DAA08765.1; -; Genomic_DNA
PIRA32366; HHBYK2; .
RefSeqNP_012500.1; NM_001181468.1; .
PDB3H0X; X-ray; 1.92 A; A=438-586
PDB3QFP; X-ray; 2.26 A; A=43-426
PDB3QFU; X-ray; 1.80 A; A=43-426
PDB3QML; X-ray; 2.31 A; A/B=43-426
PDBsum3H0X; -; .
PDBsum3QFP; -; .
PDBsum3QFU; -; .
PDBsum3QML; -; .
ProteinModelPortalP16474; -; .
SMRP16474; 48-656; .
DIPDIP-2392N; -; .
IntActP16474; 76; .
MINTMINT-506436; -; .
STRINGP16474; -; .
SWISS-2DPAGEP16474; -; .
PeptideAtlasP16474; -; .
EnsemblFungiYJL034W; YJL034W; YJL034W; .
GeneID853418; -; .
KEGGsce:YJL034W; -; .
NMPDRfig|4932.3.peg.3474; -; .
CYGDYJL034w; -; .
SGDS000003571; KAR2; .
eggNOGfuNOG07698; -; .
GeneTreeEFGT00050000000911; -; .
HOGENOMHBG334976; -; .
OMADIVANDQ; -; .
OrthoDBEOG4X0R1M; -; .
PhylomeDBP16474; -; .
NextBio973939; -; .
ArrayExpressP16474; -; .
GenevestigatorP16474; -; .
GermOnlineYJL034W; Saccharomyces cerevisiae; .
GOGO:0034099; C:luminal surveillance complex; IDA:SGD; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0016887; F:ATPase activity; IDA:SGD; .
GOGO:0051082; F:unfolded protein binding; IMP:SGD; .
GOGO:0030433; P:ER-associated protein catabolic process; IMP:SGD; .
GOGO:0000742; P:karyogamy involved in conjugation with cellular fusion; IGI:SGD; .
GOGO:0031204; P:posttranslational protein targeting to membrane; translocation; IDA:SGD
GOGO:0006986; P:response to unfolded protein; IMP:SGD; .
GOGO:0006616; P:SRP-dependent cotranslational protein targeting to membrane; translocation; IMP:SGD
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
PROSITEPS00014; ER_TARGET; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server