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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P16659




SWISS-2DPAGE:  P16659


P16659


General information about the entry
View entry in simple text format
Entry nameSYP_ECOLI
Primary accession numberP16659
integrated into SWISS-2DPAGE on October 1, 1998 (release 8)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Prolyl-tRNA synthetase; EC=6.1.1.15; AltName: Full=Global RNA synthesis factor; AltName: Full=Proline--tRNA ligase; Short=ProRS;.
Gene nameName=proS
Synonyms=drpA
OrderedLocusNames=b0194, JW0190
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.06; Mw=55738  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP16659; SYP_ECOLI.
UniProtKB/Swiss-ProtP16659; SYP_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameSYP_ECOLI
Primary accession numberP16659
Secondary accession number(s) P78272 Q59430
Sequence was last modified on August 29, 2003 (version 4)
Annotations were last modified on October 19, 2011 (version 119)
Name and origin of the protein
DescriptionRecName: Full=Prolyl-tRNA synthetase; EC=6.1.1.15; AltName: Full=Global RNA synthesis factor; AltName: Full=Proline--tRNA ligase; Short=ProRS;
Gene nameName=proS
Synonyms=drpA
OrderedLocusNames=b0194, JW0190
Encoded onName=proS; Synonyms=drpA; OrderedLocusNames=b0194, JW0190
KeywordsAminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX55518; CAA39134.1; -; Genomic_DNA
EMBLM97858; AAA24420.1; -; Genomic_DNA
EMBLM32357; AAA23710.1; -; Genomic_DNA
EMBLU70214; AAB08622.1; -; Genomic_DNA
EMBLU00096; AAC73305.1; -; Genomic_DNA
EMBLAP009048; BAA77870.2; -; Genomic_DNA
EMBLD15061; BAA03654.1; -; Genomic_DNA
PIRB64744; YPEC; .
RefSeqNP_414736.1; NC_000913.2; .
ProteinModelPortalP16659; -; .
SMRP16659; 1-571; .
DIPDIP-10573N; -; .
IntActP16659; 20; .
MINTMINT-1261583; -; .
SWISS-2DPAGEP16659; -; .
2DBase-EcoliP16659; -; .
PRIDEP16659; -; .
EnsemblBacteriaEBESCT00000004981; EBESCP00000004981; EBESCG00000004066; .
EnsemblBacteriaEBESCT00000015915; EBESCP00000015206; EBESCG00000014975; .
GeneID949116; -; .
GenomeReviewsAP009048_GR; JW0190; .
GenomeReviewsU00096_GR; b0194; .
KEGGecj:JW0190; -; .
KEGGeco:b0194; -; .
EchoBASEEB0763; -; .
EcoGeneEG10770; proS; .
eggNOGCOG0442; -; .
GeneTreeEBGT00050000008980; -; .
HOGENOMHBG403504; -; .
OMAIQPAELW; -; .
ProtClustDBPRK09194; -; .
BioCycEcoCyc:PROS-MONOMER; -; .
BioCycMetaCyc:PROS-MONOMER; -; .
GenevestigatorP16659; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0043906; F:Ala-tRNA(Pro) hydrolase activity; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004827; F:proline-tRNA ligase activity; IDA:EcoCyc; .
GOGO:0006433; P:prolyl-tRNA aminoacylation; IMP:EcoCyc; .
HAMAPMF_01569; Pro_tRNA_synth_type1; 1; -
InterProIPR002314; aa-tRNA-synt_IIb_cons-dom; .
InterProIPR006195; aa-tRNA-synth_II; .
InterProIPR004154; Anticodon-bd; .
InterProIPR002316; Pro-tRNA-synth_IIa; .
InterProIPR004500; Pro-tRNA-synth_IIa_bac-type; .
InterProIPR023717; Pro-tRNA-Synthase_IIa_type1; .
InterProIPR007214; YbaK/aa-tRNA-synth-assoc-dom; .
Gene3DG3DSA:3.40.50.800; Anticodon_bd; 1; .
Gene3DG3DSA:3.90.960.10; YbaK/aa-tRNA-synth-assoc-reg; 1; .
PANTHERPTHR11451:SF3; PTHR11451:SF3; 1; .
PfamPF03129; HGTP_anticodon; 1; .
PfamPF00587; tRNA-synt_2b; 1; .
PfamPF04073; YbaK; 1; .
PRINTSPR01046; TRNASYNTHPRO; .
SUPFAMSSF52954; Anticodon_bd; 1; .
SUPFAMSSF55826; YbaK/aa-tRNA-synth-assoc-reg; 1; .
TIGRFAMsTIGR00409; ProS_fam_II; 1; .
PROSITEPS50862; AA_TRNA_LIGASE_II; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server