World-2DPAGE Home
Home  |  Contact

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P16703




SWISS-2DPAGE:  P16703


P16703


General information about the entry
View entry in simple text format
Entry nameCYSM_ECOLI
Primary accession numberP16703
integrated into SWISS-2DPAGE on June 30, 1999 (release 10)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Cysteine synthase B; Short=CSase B; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase B; Short=OAS-TL B; AltName: Full=O-acetylserine sulfhydrylase B;.
Gene nameName=cysM
OrderedLocusNames=b2421, JW2414
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.47; Mw=31435  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP16703; CYSM_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCYSM_ECOLI
Primary accession numberP16703
Sequence was last modified on August 1, 1990 (version 1)
Annotations were last modified on October 19, 2011 (version 116)
Name and origin of the protein
DescriptionRecName: Full=Cysteine synthase B; Short=CSase B; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase B; Short=OAS-TL B; AltName: Full=O-acetylserine sulfhydrylase B;
Gene nameName=cysM
OrderedLocusNames=b2421, JW2414
Encoded onName=cysM; OrderedLocusNames=b2421, JW2414
Keywords3D-structure; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM32101; AAA23640.1; -; Genomic_DNA
EMBLU00096; AAC75474.1; -; Genomic_DNA
EMBLAP009048; BAA16295.1; -; Genomic_DNA
PIRD35402; SYECBC; .
RefSeqNP_416916.1; NC_000913.2; .
PDB2BHS; X-ray; 2.67 A; A/B/C/D=1-303
PDB2BHT; X-ray; 2.10 A; A/B/C/D=1-303
PDB2V03; X-ray; 1.33 A; A=1-303
PDBsum2BHS; -; .
PDBsum2BHT; -; .
PDBsum2V03; -; .
ProteinModelPortalP16703; -; .
SMRP16703; 2-293; .
DIPDIP-9383N; -; .
IntActP16703; 9; .
MINTMINT-1300981; -; .
SWISS-2DPAGEP16703; -; .
EnsemblBacteriaEBESCT00000004848; EBESCP00000004848; EBESCG00000003956; .
EnsemblBacteriaEBESCT00000014906; EBESCP00000014197; EBESCG00000013966; .
GeneID946888; -; .
GenomeReviewsAP009048_GR; JW2414; .
GenomeReviewsU00096_GR; b2421; .
KEGGecj:JW2414; -; .
KEGGeco:b2421; -; .
EchoBASEEB0190; -; .
EcoGeneEG10193; cysM; .
eggNOGCOG0031; -; .
GeneTreeEBGT00050000009701; -; .
HOGENOMHBG748215; -; .
OMATIEACIG; -; .
ProtClustDBPRK11761; -; .
BioCycEcoCyc:ACSERLYB-MONOMER; -; .
BioCycMetaCyc:ACSERLYB-MONOMER; -; .
GenevestigatorP16703; -; .
GOGO:0004124; F:cysteine synthase activity; IDA:EcoCyc; .
GOGO:0080146; F:L-cysteine desulfhydrase activity; IMP:EcoCyc; .
GOGO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GOGO:0016740; F:transferase activity; IEA:UniProtKB-KW; .
GOGO:0006535; P:cysteine biosynthetic process from serine; IDA:EcoCyc; .
InterProIPR001216; Cys_synth_BS; .
InterProIPR005856; Cys_synthKM; .
InterProIPR005858; CysM; .
InterProIPR001926; PyrdxlP-dep_enz_bsu; .
PfamPF00291; PALP; 1; .
SUPFAMSSF53686; PyrdxlP-dep_enz_bsu; 1; .
TIGRFAMsTIGR01136; CysKM; 1; .
TIGRFAMsTIGR01138; CysM; 1; .
PROSITEPS00901; CYS_SYNTHASE; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server