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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P16858




SWISS-2DPAGE:  P16858


P16858


General information about the entry
View entry in simple text format
Entry nameG3P_MOUSE
Primary accession numberP16858
integrated into SWISS-2DPAGE on October 31, 1999 (release 11)
2D Annotations were last modified onOctober 1, 2001 (version 2)
General Annotations were last modified on May 19, 2011 (version 9)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;.
Gene nameName=Gapdh
Synonyms=Gapd
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
[2]   MAPPING ON GEL
PubMed=11503206; [NCBI, ExPASy, EBI, Israel, Japan]
Lanne B., Potthast F., Hoglund A., Brockenhuus Von Lowenhielm H., Nystrom A.-C., Nilsson F., Dahllof B.
''''''Thiourea enhances mapping of the proteome from murine white adipose tissue'';'';''
Proteomics 1(1):819-828(2001)
[3]   MAPPING ON GEL
Tonella L., Hoogland C., Sanchez J.-C.
Submitted (OCT-2001) to SWISS-2DPAGE
2D PAGE maps for identified proteins
How to interpret a protein

MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=8.32; Mw=35785  [identification data]
pI=7.31; Mw=35389  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=8.35; Mw=38472

MAPPING (identification):
GEL MATCHING [3] WITH WAT_MOUSE 2-DE MAP FROM LANNE ET AL. [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEP16858; P16858.
UniProtKB/Swiss-ProtP16858; G3P_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameG3P_MOUSE
Primary accession numberP16858
Secondary accession number(s) A6H6A8 Q0QEU0 Q3THM2 Q3TUI2 Q3UMT2 Q4V783 Q569X2 Q569X5 Q5U410
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on September 21, 2011 (version 122)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;
Gene nameName=Gapdh
Synonyms=Gapd
Encoded onName=Gapdh; Synonyms=Gapd
KeywordsAcetylation; ADP-ribosylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Methylation; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; S-nitrosylation; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM32599; AAA37659.1; -; mRNA
EMBLAK002273; BAB21979.1; -; mRNA
EMBLAK081405; BAC38211.1; -; mRNA
EMBLAK140794; BAE24481.1; -; mRNA
EMBLAK144690; BAE26016.1; -; mRNA
EMBLAK146435; BAE27169.1; -; mRNA
EMBLAK147738; BAE28105.1; -; mRNA
EMBLAK147891; BAE28208.1; -; mRNA
EMBLAK160399; BAE35768.1; -; mRNA
EMBLAK160753; BAE35989.1; -; mRNA
EMBLAK164415; BAE37778.1; -; mRNA
EMBLAK168217; BAE40174.1; -; mRNA
EMBLAL662926; CAI25599.1; -; Genomic_DNA
EMBLBC082592; AAH82592.1; -; mRNA
EMBLBC083065; AAH83065.1; -; mRNA
EMBLBC083079; AAH83079.1; -; mRNA
EMBLBC083080; AAH83080.1; -; mRNA
EMBLBC083149; AAH83149.1; -; mRNA
EMBLBC085274; AAH85274.1; -; mRNA
EMBLBC085275; AAH85275.1; -; mRNA
EMBLBC085315; AAH85315.1; -; mRNA
EMBLBC091768; AAH91768.1; -; mRNA
EMBLBC092252; AAH92252.1; -; mRNA
EMBLBC092264; AAH92264.1; -; mRNA
EMBLBC092267; AAH92267.1; -; mRNA
EMBLBC092294; AAH92294.1; -; mRNA
EMBLBC093508; AAH93508.1; -; mRNA
EMBLBC094037; AAH94037.1; -; mRNA
EMBLBC095932; AAH95932.1; -; mRNA
EMBLBC096440; AAH96440.1; -; mRNA
EMBLBC096590; AAH96590.1; -; mRNA
EMBLBC110311; AAI10312.1; -; mRNA
EMBLBC145810; AAI45811.1; -; mRNA
EMBLBC145812; AAI45813.1; -; mRNA
EMBLDQ403054; ABD77187.1; -; mRNA
IPIIPI00273646; -; .
PIRJT0553; DEMSG; .
RefSeqNP_032110.1; NM_008084.2; .
RefSeqXP_001476757.1; XM_001476707.2; .
UniGeneMm.304088; -; .
UniGeneMm.309092; -; .
UniGeneMm.317779; -; .
UniGeneMm.343110; -; .
UniGeneMm.392463; -; .
UniGeneMm.392480; -; .
UniGeneMm.414470; -; .
UniGeneMm.458138; -; .
UniGeneMm.458416; -; .
ProteinModelPortalP16858; -; .
SMRP16858; 2-333; .
IntActP16858; 7; .
MINTMINT-1869564; -; .
STRINGP16858; -; .
PhosphoSiteP16858; -; .
SWISS-2DPAGEP16858; -; .
REPRODUCTION-2DPAGEP16858; -; .
REPRODUCTION-2DPAGEQ5U410; -; .
PRIDEP16858; -; .
EnsemblENSMUST00000098560; ENSMUSP00000100572; ENSMUSG00000078162; .
EnsemblENSMUST00000104945; ENSMUSP00000100550; ENSMUSG00000078142; .
EnsemblENSMUST00000109588; ENSMUSP00000105217; ENSMUSG00000078965; .
EnsemblENSMUST00000109595; ENSMUSP00000105224; ENSMUSG00000078967; .
EnsemblENSMUST00000110458; ENSMUSP00000128664; ENSMUSG00000072451; .
EnsemblENSMUST00000113842; ENSMUSP00000109473; ENSMUSG00000079501; .
EnsemblENSMUST00000117757; ENSMUSP00000113942; ENSMUSG00000057666; .
EnsemblENSMUST00000118875; ENSMUSP00000113213; ENSMUSG00000057666; .
GeneID100042025; -; .
GeneID14433; -; .
KEGGmmu:100042025; -; .
KEGGmmu:14433; -; .
CTD2597; -; .
MGIMGI:95640; Gapdh; .
eggNOGroNOG06117; -; .
HOVERGENHBG000227; -; .
InParanoidP16858; -; .
OrthoDBEOG4Q84XS; -; .
PhylomeDBP16858; -; .
NextBio447908; -; .
BgeeP16858; -; .
CleanExMM_GAPDH; -; .
GenevestigatorP16858; -; .
GermOnlineENSMUSG00000057666; Mus musculus; .
GOGO:0005829; C:cytosol; ISS:UniProtKB; .
GOGO:0005634; C:nucleus; ISS:UniProtKB; .
GOGO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; ISS:UniProtKB; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0035605; F:peptidyl-cysteine S-nitrosylase activity; ISS:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
GOGO:0051402; P:neuron apoptosis; ISS:UniProtKB; .
GOGO:0035606; P:peptidyl-cysteine S-trans-nitrosylation; ISS:UniProtKB; .
GOGO:0050821; P:protein stabilization; ISS:UniProtKB; .
InterProIPR020831; GlycerAld/Erythrose_P_DH; .
InterProIPR020830; GlycerAld_3-P_DH_AS; .
InterProIPR020829; GlycerAld_3-P_DH_cat; .
InterProIPR020828; GlycerAld_3-P_DH_NAD(P)-bd; .
InterProIPR006424; Glyceraldehyde-3-P_DH_1; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PANTHERPTHR10836; GAP_DH; 1; .
PfamPF02800; Gp_dh_C; 1; .
PfamPF00044; Gp_dh_N; 1; .
PIRSFPIRSF000149; GAP_DH; 1; .
PRINTSPR00078; G3PDHDRGNASE; .
SMARTSM00846; Gp_dh_N; 1; .
TIGRFAMsTIGR01534; GAPDH-I; 1; .
PROSITEPS00071; GAPDH; 1; .



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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server