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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P27616




SWISS-2DPAGE:  P27616


P27616


General information about the entry
View entry in simple text format
Entry namePUR7_YEAST
Primary accession numberP27616
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 9)
Name and origin of the protein
DescriptionRecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase; EC=6.3.2.6; AltName: Full=SAICAR synthetase;.
Gene nameName=ADE1
OrderedLocusNames=YAR015W
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=95203288; PubMed=7895733; [NCBI, ExPASy, EBI, Israel, Japan]
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.
''''''Protein identifications for a Saccharomyces cerevisiae protein database'';'';''
Electrophoresis 15(1):1466-1486(1994)
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=5.64; Mw=32116

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED BY GARRELS [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP27616; PUR7_YEAST.
YEPD7290.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePUR7_YEAST
Primary accession numberP27616
Secondary accession number(s) D6VPM4
Sequence was last modified on October 5, 2010 (version 2)
Annotations were last modified on October 19, 2011 (version 116)
Name and origin of the protein
DescriptionRecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase; EC=6.3.2.6; AltName: Full=SAICAR synthetase;
Gene nameName=ADE1
OrderedLocusNames=YAR015W
Encoded onName=ADE1; OrderedLocusNames=YAR015W
Keywords3D-structure; Acetylation; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Phosphoprotein; Purine biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM61209; AAA34396.1; -; Genomic_DNA
EMBLM67445; AAA34398.1; -; Genomic_DNA
EMBLL22015; AAC04963.1; -; Genomic_DNA
EMBLX60549; CAA43043.1; -; Genomic_DNA
EMBLBK006935; DAA06994.1; -; Genomic_DNA
PIRS20122; JQ1395; .
RefSeqNP_009409.1; NM_001178216.1; .
PDB1A48; X-ray; 1.90 A; A=2-306
PDB1OBD; X-ray; 1.40 A; A=2-306
PDB1OBG; X-ray; 2.05 A; A=2-306
PDB2CNQ; X-ray; 1.00 A; A=2-306
PDB2CNU; X-ray; 1.05 A; A=2-306
PDB2CNV; X-ray; 2.00 A; A=2-306
PDBsum1A48; -; .
PDBsum1OBD; -; .
PDBsum1OBG; -; .
PDBsum2CNQ; -; .
PDBsum2CNU; -; .
PDBsum2CNV; -; .
ProteinModelPortalP27616; -; .
SMRP27616; 2-306; .
IntActP27616; 2; .
MINTMINT-659098; -; .
STRINGP27616; -; .
SWISS-2DPAGEP27616; -; .
PeptideAtlasP27616; -; .
EnsemblFungiYAR015W; YAR015W; YAR015W; .
GeneID851272; -; .
KEGGsce:YAR015W; -; .
SGDS000000070; ADE1; .
eggNOGfuNOG04520; -; .
GeneTreeEFGT00050000005120; -; .
HOGENOMHBG555040; -; .
OMALTLMDEV; -; .
OrthoDBEOG4JMBZN; -; .
PhylomeDBP27616; -; .
BioCycMetaCyc:MONOMER-488; -; .
DrugBankDB00131; Adenosine monophosphate; .
ArrayExpressP27616; -; .
GenevestigatorP27616; -; .
GermOnlineYAR015W; Saccharomyces cerevisiae; .
GOGO:0005737; C:cytoplasm; IDA:SGD; .
GOGO:0005634; C:nucleus; IDA:SGD; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IDA:SGD; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0006189; P:'de novo' IMP biosynthetic process; IDA:SGD; .
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001636; SAICAR_synth; .
InterProIPR018236; SAICAR_synthetase_CS; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
PANTHERPTHR11609; SAICAR_synt; 1; .
PfamPF01259; SAICAR_synt; 1; .
TIGRFAMsTIGR00081; PurC; 1; .
PROSITEPS01057; SAICAR_SYNTHETASE_1; 1; .
PROSITEPS01058; SAICAR_SYNTHETASE_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server