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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P30613




SWISS-2DPAGE:  P30613


P30613


General information about the entry
View entry in simple text format
Entry nameKPYR_HUMAN
Primary accession numberP30613
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate kinase isozymes R/L; EC=2.7.1.40; AltName: Full=Pyruvate kinase 1; AltName: Full=R-type/L-type pyruvate kinase; AltName: Full=Red cell/liver pyruvate kinase;.
Gene nameName=PKLR
Synonyms=PK1, PKL
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=79188222; PubMed=109131; [NCBI, ExPASy, EBI, Israel, Japan]
Edwards J., Anderson N.G., Nance S.L., Anderson N.L.
''''''Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins'';'';''
Blood 53(1):1121-1132(1979)
[2]   MAPPING ON GEL
MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.
''''''Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis'';'';''
Electrophoresis 16(1):1152-1159(1995)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

PLATELET_HUMAN {Platelet}
Homo sapiens (Human)
Tissue: Platelet
PLATELET_HUMAN
  map experimental info
  protein estimated location
 
PLATELET_HUMAN

MAP LOCATIONS:
pI=5.27; Mw=66362
pI=5.30; Mw=66006
pI=5.24; Mw=66362

MAPPING (identification):
MATCHING WITH THE RBC MASTER GEL [2].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=5.24; Mw=74285
pI=5.29; Mw=73694

PATHOLOGICAL LEVEL:
DECREASED DURING HEREDITARY HEMOLYTIC ANEMIA.

POSITIONAL VARIANTS:
TOKYO AND FUKUSHIMA/MAEBASHI VARIANTS.

MAPPING (identification):
IMMUNOBLOTTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEP30613; P30613.
UniProtKB/Swiss-ProtP30613; KPYR_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameKPYR_HUMAN
Primary accession numberP30613
Secondary accession number(s) P11973
Sequence was last modified on May 30, 2000 (version 2)
Annotations were last modified on October 19, 2011 (version 143)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate kinase isozymes R/L; EC=2.7.1.40; AltName: Full=Pyruvate kinase 1; AltName: Full=R-type/L-type pyruvate kinase; AltName: Full=Red cell/liver pyruvate kinase;
Gene nameName=PKLR
Synonyms=PK1, PKL
Encoded onName=PKLR; Synonyms=PK1, PKL
Keywords3D-structure; Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Disease mutation; Glycolysis; Hereditary hemolytic anemia; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Potassium; Pyruvate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB015983; BAA31706.1; -; mRNA
EMBLM15465; AAA60104.1; -; mRNA
EMBLAY316591; AAP69527.1; -; Genomic_DNA
EMBLBC025737; AAH25737.1; -; mRNA
EMBLS60712; AAB26262.1; -; mRNA
IPIIPI00027165; -; .
IPIIPI00941093; -; .
PIRI52269; KIHUPR; .
RefSeqNP_000289.1; NM_000298.5; .
RefSeqNP_870986.1; NM_181871.3; .
UniGeneHs.95990; -; .
PDB2VGB; X-ray; 2.73 A; A/B/C/D=47-574
PDB2VGF; X-ray; 2.75 A; A/B/C/D=47-574
PDB2VGG; X-ray; 2.74 A; A/B/C/D=47-574
PDB2VGI; X-ray; 2.87 A; A/B/C/D=47-574
PDBsum2VGB; -; .
PDBsum2VGF; -; .
PDBsum2VGG; -; .
PDBsum2VGI; -; .
ProteinModelPortalP30613; -; .
SMRP30613; 57-573; .
IntActP30613; 4; .
STRINGP30613; -; .
PhosphoSiteP30613; -; .
SWISS-2DPAGEP30613; -; .
REPRODUCTION-2DPAGEP30613; -; .
PRIDEP30613; -; .
EnsemblENST00000271946; ENSP00000271946; ENSG00000143627; .
EnsemblENST00000342741; ENSP00000339933; ENSG00000143627; .
GeneID5313; -; .
KEGGhsa:5313; -; .
UCSCuc001fkb.2; human; .
CTD5313; -; .
GeneCardsGC01M126621; -; .
H-InvDBHIX0001126; -; .
HGNCHGNC:9020; PKLR; .
HPAHPA011991; -; .
MIM102900; phenotype; .
MIM266200; phenotype; .
MIM609712; gene; .
neXtProtNX_P30613; -; .
Orphanet766; Hemolytic anemia due to red cell pyruvate kinase deficiency; .
PharmGKBPA33352; -; .
GeneTreeENSGT00390000008859; -; .
HOVERGENHBG000941; -; .
PhylomeDBP30613; -; .
Pathway_Interaction_DBhnf3bpathway; FOXA2 and FOXA3 transcription factor networks; .
ReactomeREACT_13698; Regulation of beta-cell development; .
ReactomeREACT_1505; Integration of energy metabolism; .
ReactomeREACT_474; Metabolism of carbohydrates; .
DrugBankDB00119; Pyruvic acid; .
NextBio20542; -; .
PMAP-CutDBP30613; -; .
ArrayExpressP30613; -; .
BgeeP30613; -; .
CleanExHS_PKLR; -; .
GenevestigatorP30613; -; .
GermOnlineENSG00000143627; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0030955; F:potassium ion binding; IEA:InterPro; .
GOGO:0004743; F:pyruvate kinase activity; TAS:ProtInc; .
GOGO:0031018; P:endocrine pancreas development; TAS:Reactome; .
GOGO:0006112; P:energy reserve metabolic process; TAS:Reactome; .
GOGO:0006096; P:glycolysis; TAS:Reactome; .
GOGO:0031325; P:positive regulation of cellular metabolic process; TAS:Reactome; .
InterProIPR001697; Pyr_Knase; .
InterProIPR015813; Pyrv/PenolPyrv_Kinase; .
InterProIPR011037; Pyrv_Knase-like_insert_dom; .
InterProIPR015794; Pyrv_Knase_a/b; .
InterProIPR018209; Pyrv_Knase_AS; .
InterProIPR015793; Pyrv_Knase_brl; .
InterProIPR015795; Pyrv_Knase_C; .
InterProIPR015806; Pyrv_Knase_insert_dom; .
Gene3DG3DSA:2.40.33.10; PK_B_barrel_like; 1; .
Gene3DG3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; .
Gene3DG3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; .
PANTHERPTHR11817; Pyruvate_kinase; 1; .
PfamPF00224; PK; 1; .
PfamPF02887; PK_C; 1; .
PRINTSPR01050; PYRUVTKNASE; .
SUPFAMSSF50800; PK_B_barrel_like; 1; .
SUPFAMSSF52935; Pyruvate_kinase; 1; .
SUPFAMSSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMsTIGR01064; Pyruv_kin; 1; .
PROSITEPS00110; PYRUVATE_KINASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server