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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P30859




SWISS-2DPAGE:  P30859


P30859


General information about the entry
View entry in simple text format
Entry nameARTI_ECOLI
Primary accession numberP30859
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Putative ABC transporter arginine-binding protein 2; Flags: Precursor;.
Gene nameName=artI
OrderedLocusNames=b0863, JW0847
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[2]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[3]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.23; Mw=26630

MAPPING (identification):
AMINO ACID COMPOSITION AND SEQUENCE TAG (AETI) [1].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.07; Mw=31478  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.27; Mw=24964  [identification data]

EXPRESSION:
increase after benzoic acid treatment [3].

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP30859; ARTI_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameARTI_ECOLI
Primary accession numberP30859
Secondary accession number(s) P77640
Sequence was last modified on November 1, 1997 (version 3)
Annotations were last modified on October 19, 2011 (version 91)
Name and origin of the protein
DescriptionRecName: Full=Putative ABC transporter arginine-binding protein 2; Flags: Precursor;
Gene nameName=artI
OrderedLocusNames=b0863, JW0847
Encoded onName=artI; OrderedLocusNames=b0863, JW0847
KeywordsAmino-acid transport; Complete proteome; Direct protein sequencing; Periplasm; Reference proteome; Signal; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX86160; CAA60102.1; -; Genomic_DNA
EMBLU00096; AAC73950.1; -; Genomic_DNA
EMBLAP009048; BAA35577.1; -; Genomic_DNA
PIRG64824; G64824; .
RefSeqNP_415384.1; NC_000913.2; .
ProteinModelPortalP30859; -; .
SMRP30859; 19-243; .
IntActP30859; 3; .
TCDB3.A.1.3.3; ATP-binding cassette (ABC) superfamily; .
SWISS-2DPAGEP30859; -; .
EnsemblBacteriaEBESCT00000001514; EBESCP00000001514; EBESCG00000001260; .
EnsemblBacteriaEBESCT00000017016; EBESCP00000016307; EBESCG00000016075; .
GeneID948988; -; .
GenomeReviewsAP009048_GR; JW0847; .
GenomeReviewsU00096_GR; b0863; .
KEGGecj:JW0847; -; .
KEGGeco:b0863; -; .
EchoBASEEB1582; -; .
EcoGeneEG11625; artI; .
eggNOGCOG0834; -; .
GeneTreeEBGT00050000009492; -; .
HOGENOMHBG698334; -; .
OMAVIAGMDI; -; .
ProtClustDBPRK15007; -; .
BioCycEcoCyc:ARTI-MONOMER; -; .
GenevestigatorP30859; -; .
GOGO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro; .
GOGO:0005215; F:transporter activity; IEA:InterPro; .
GOGO:0006865; P:amino acid transport; IEA:UniProtKB-KW; .
InterProIPR005768; Lys_Arg_Orn-bd; .
InterProIPR001638; SBP_bac_3; .
InterProIPR018313; SBP_bac_3_CS; .
PfamPF00497; SBP_bac_3; 1; .
SMARTSM00062; PBPb; 1; .
TIGRFAMsTIGR01096; 3A0103s03R; 1; .
PROSITEPS01039; SBP_BACTERIAL_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server