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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P32324




SWISS-2DPAGE:  P32324


P32324


General information about the entry
View entry in simple text format
Entry nameEF2_YEAST
Primary accession numberP32324
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor 2; Short=EF-2; AltName: Full=Eukaryotic elongation factor 2; Short=eEF2; AltName: Full=Ribosomal translocase; AltName: Full=Translation elongation factor 2;.
Gene nameName=EFT1
OrderedLocusNames=YOR133W
ORFNames=O3317, YOR3317W
   and
Name=EFT2
OrderedLocusNames=YDR385W
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=95203288; PubMed=7895733; [NCBI, ExPASy, EBI, Israel, Japan]
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.
''''''Protein identifications for a Saccharomyces cerevisiae protein database'';'';''
Electrophoresis 15(1):1466-1486(1994)
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=6.20; Mw=98328
pI=5.92; Mw=98052
pI=6.26; Mw=98052

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED BY GARRELS [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP32324; EF2_YEAST.
YEPD5840.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameEF2_YEAST
Primary accession numberP32324
Secondary accession number(s) D6VT19 Q6JEF7
Sequence was last modified on October 1, 1993 (version 1)
Annotations were last modified on October 19, 2011 (version 126)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor 2; Short=EF-2; AltName: Full=Eukaryotic elongation factor 2; Short=eEF2; AltName: Full=Ribosomal translocase; AltName: Full=Translation elongation factor 2;
Gene nameName=EFT1
OrderedLocusNames=YOR133W
ORFNames=O3317, YOR3317W
   and
Name=EFT2
OrderedLocusNames=YDR385W
Encoded onName=EFT1; OrderedLocusNames=YOR133W; ORFNames=O3317, YOR3317W; and Name=EFT2; OrderedLocusNames=YDR385W
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; GTP-binding; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; rRNA-binding.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM59369; AAA21646.1; -; Genomic_DNA
EMBLM59370; AAA51398.1; -; Genomic_DNA
EMBLZ75041; CAA99332.1; -; Genomic_DNA
EMBLU32274; AAB64827.1; -; Genomic_DNA
EMBLU28373; AAB64821.1; -; Genomic_DNA
EMBLX90518; CAA62116.1; -; Genomic_DNA
EMBLX94335; CAA64052.1; -; Genomic_DNA
EMBLAY497635; AAT12549.1; -; Genomic_DNA
EMBLBK006948; DAA10907.1; -; Genomic_DNA
EMBLBK006938; DAA12229.1; -; Genomic_DNA
PIRA41778; A41778; .
RefSeqNP_010673.1; NM_001180693.1; .
RefSeqNP_014776.1; NM_001183552.1; .
PDB1N0U; X-ray; 2.12 A; A=1-842
PDB1N0V; X-ray; 2.85 A; C/D=1-842
PDB1S1H; EM; 11.70 A; T=1-842
PDB1U2R; X-ray; 2.60 A; A=1-842
PDB1ZM2; X-ray; 3.07 A; A/C/E=1-842
PDB1ZM3; X-ray; 3.07 A; A/C/E=1-842
PDB1ZM4; X-ray; 2.90 A; A/C/E=1-842
PDB1ZM9; X-ray; 2.80 A; A/C/E=1-842
PDB2E1R; X-ray; 3.15 A; A=1-842
PDB2NPF; X-ray; 2.90 A; A/B=1-842
PDB2P8W; EM; 11.30 A; T=1-842
PDB2P8X; EM; 9.70 A; T=1-842
PDB2P8Y; EM; 11.70 A; T=1-842
PDB2P8Z; EM; 8.90 A; T=1-842
PDB2ZIT; X-ray; 3.00 A; A/C/E=1-842
PDB3B78; X-ray; 2.50 A; A/C/E=1-842
PDB3B82; X-ray; 2.35 A; A/C/E=1-842
PDB3B8H; X-ray; 2.50 A; A/C/E=1-842
PDB3DNY; EM; 12.60 A; T=1-842
PDBsum1N0U; -; .
PDBsum1N0V; -; .
PDBsum1S1H; -; .
PDBsum1U2R; -; .
PDBsum1ZM2; -; .
PDBsum1ZM3; -; .
PDBsum1ZM4; -; .
PDBsum1ZM9; -; .
PDBsum2E1R; -; .
PDBsum2NPF; -; .
PDBsum2P8W; -; .
PDBsum2P8X; -; .
PDBsum2P8Y; -; .
PDBsum2P8Z; -; .
PDBsum2ZIT; -; .
PDBsum3B78; -; .
PDBsum3B82; -; .
PDBsum3B8H; -; .
PDBsum3DNY; -; .
ProteinModelPortalP32324; -; .
SMRP32324; 2-842; .
DIPDIP-4911N; -; .
IntActP32324; 133; .
MINTMINT-516631; -; .
STRINGP32324; -; .
SWISS-2DPAGEP32324; -; .
EnsemblFungiYDR385W; YDR385W; YDR385W; .
EnsemblFungiYOR133W; YOR133W; YOR133W; .
GeneID851993; -; .
GeneID854301; -; .
KEGGsce:YDR385W; -; .
KEGGsce:YOR133W; -; .
CYGDYDR385w; -; .
CYGDYOR133w; -; .
SGDS000005659; EFT1; .
SGDS000002793; EFT2; .
eggNOGfuNOG07681; -; .
GeneTreeEFGT00050000001544; -; .
HOGENOMHBG737692; -; .
OMAMVNFTVE; -; .
OrthoDBEOG4PZNFQ; -; .
PhylomeDBP32324; -; .
NextBio970163; -; .
GenevestigatorP32324; -; .
GermOnlineYDR385W; Saccharomyces cerevisiae; .
GermOnlineYOR133W; Saccharomyces cerevisiae; .
GOGO:0005840; C:ribosome; TAS:SGD; .
GOGO:0005525; F:GTP binding; IEA:UniProtKB-KW; .
GOGO:0003924; F:GTPase activity; IEA:InterPro; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0019843; F:rRNA binding; IEA:UniProtKB-KW; .
GOGO:0003746; F:translation elongation factor activity; TAS:SGD; .
GOGO:0045901; P:positive regulation of translational elongation; IMP:SGD; .
InterProIPR009022; Elongation_fac_G/III/V; .
InterProIPR000795; ProtSyn_GTP-bd; .
InterProIPR020568; Ribosomal_S5_D2-typ_fold; .
InterProIPR014721; Ribosomal_S5_D2-typ_fold_subgr; .
InterProIPR005225; Small_GTP-bd_dom; .
InterProIPR000640; Transl_elong_EFG/EF2_C; .
InterProIPR005517; Transl_elong_EFG/EF2_IV; .
InterProIPR004161; Transl_elong_EFTu/EF1A_2; .
InterProIPR009000; Transl_elong_init/rib_B-barrel; .
Gene3DG3DSA:3.30.230.10; Ribosomal_S5_D2-type_fold; 1; .
Gene3DG3DSA:3.30.70.240; Transl_elong_EFG/EF2_C; 1; .
PfamPF00679; EFG_C; 1; .
PfamPF03764; EFG_IV; 1; .
PfamPF00009; GTP_EFTU; 1; .
PfamPF03144; GTP_EFTU_D2; 1; .
PRINTSPR00315; ELONGATNFCT; .
SMARTSM00838; EFG_C; 1; .
SMARTSM00889; EFG_IV; 1; .
SUPFAMSSF54980; EFG_III_V; 2; .
SUPFAMSSF54211; Ribosomal_S5_D2-typ_fold; 1; .
SUPFAMSSF50447; Translat_factor; 1; .
TIGRFAMsTIGR00231; Small_GTP; 1; .
PROSITEPS00301; EFACTOR_GTP; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server