World-2DPAGE Home
Home  |  Contact


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P38647




SWISS-2DPAGE:  P38647


P38647


General information about the entry
View entry in simple text format
Entry nameGRP75_MOUSE
Primary accession numberP38647
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onOctober 1, 2001 (version 4)
General Annotations were last modified on May 19, 2011 (version 9)
Name and origin of the protein
DescriptionRecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=p66 MOT; Flags: Precursor;.
Gene nameName=Hspa9
Synonyms=Grp75, Hsp74, Hspa9a
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=5.31; Mw=64512
pI=5.33; Mw=64390  [identification data]
pI=5.36; Mw=64267
pI=5.46; Mw=64267
pI=5.42; Mw=64145

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [2];
SPOT 2D-001PGN: MASS MASS FINGERPRINTING [2].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=5.30; Mw=69426
pI=5.27; Mw=69426
pI=5.32; Mw=69057  [identification data]
pI=5.34; Mw=68690  [identification data]

MAPPING (identification):
SPOTS 2D-00132W, 2D-00133*: MATCHING [2] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1], MASS FINGERPRINTING AND MICROSEQUENCING [2].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=5.34; Mw=69385

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=5.31; Mw=67332
pI=5.34; Mw=67063

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP38647; GRP75_MOUSE.
REPRODUCTION-2DPAGEIPI00133903; IPI00133903.
REPRODUCTION-2DPAGEP38647; P38647.
REPRODUCTION-2DPAGEIPI00880839; IPI00880839.
UniProtKB/Swiss-ProtP38647; GRP75_MOUSE.
World-2DPAGE RepositoryP38647; GRP75_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGRP75_MOUSE
Primary accession numberP38647
Secondary accession number(s) Q9CQ05
Sequence was last modified on July 11, 2001 (version 2)
Annotations were last modified on October 19, 2011 (version 114)
Name and origin of the protein
DescriptionRecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=p66 MOT; Flags: Precursor;
Gene nameName=Hspa9
Synonyms=Grp75, Hsp74, Hspa9a
Encoded onName=Hspa9; Synonyms=Grp75, Hsp74, Hspa9a
KeywordsAcetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD11089; BAA01862.2; -; mRNA
EMBLL06896; -; NOT_ANNOTATED_CDS; mRNA
EMBLD17666; BAA04548.1; -; Genomic_DNA
EMBLD17556; BAA04493.1; -; mRNA
EMBLAK004946; BAB23690.1; -; mRNA
EMBLAK002634; BAB22248.1; -; mRNA
IPIIPI00133903; -; .
PIRS39839; A48127; .
RefSeqNP_034611.2; NM_010481.2; .
UniGeneMm.209419; -; .
ProteinModelPortalP38647; -; .
SMRP38647; 55-651; .
IntActP38647; 5; .
STRINGP38647; -; .
PhosphoSiteP38647; -; .
SWISS-2DPAGEP38647; -; .
COMPLUYEAST-2DPAGEP38647; -; .
REPRODUCTION-2DPAGEIPI00133903; -; .
REPRODUCTION-2DPAGEP38647; -; .
PRIDEP38647; -; .
GeneID15526; -; .
KEGGmmu:15526; -; .
CTD3313; -; .
MGIMGI:96245; Hspa9; .
eggNOGroNOG13746; -; .
HOGENOMHBG334976; -; .
HOVERGENHBG051845; -; .
InParanoidP38647; -; .
OrthoDBEOG49KFQ6; -; .
PhylomeDBP38647; -; .
ArrayExpressP38647; -; .
BgeeP38647; -; .
CleanExMM_HSPA9; -; .
GenevestigatorP38647; -; .
GermOnlineENSMUSG00000024359; Mus musculus; .
GOGO:0005739; C:mitochondrion; IDA:MGI; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0019899; F:enzyme binding; IPI:UniProtKB; .
GOGO:0051082; F:unfolded protein binding; IEA:InterPro; .
GOGO:0006611; P:protein export from nucleus; IDA:MGI; .
GOGO:0006457; P:protein folding; IEA:InterPro; .
InterProIPR012725; Chaperone_DnaK; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
TIGRFAMsTIGR02350; Prok_dnaK; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server