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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: P46527



General information about the entry
View entry in simple text format
Entry nameCDN1B_HUMAN
Primary accession numberP46527
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onDecember 1, 2000 (version 2)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Cyclin-dependent kinase inhibitor 1B; AltName: Full=Cyclin-dependent kinase inhibitor p27; AltName: Full=p27Kip1;.
Gene nameName=CDKN1B
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
MEDLINE=97295311; PubMed=9150953; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Wirth P., Jaccoud S., Appel R.D., Sarto C., Wilkins M.R., Hochstrasser D.F.
''''''Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots'';'';''
Electrophoresis 18(1):638-641(1997)
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
2D PAGE maps for identified proteins
How to interpret a protein

DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
  map experimental info
  protein estimated location

pI=6.31; Mw=23409
pI=6.46; Mw=23087
pI=6.86; Mw=22876

MAPPING (identification):

Homo sapiens (Human)
Tissue: Hepatoblastoma
  map experimental info
  protein estimated location

pI=6.56; Mw=25143
pI=6.96; Mw=25143
pI=6.72; Mw=24908

MAPPING (identification):

Homo sapiens (Human)
Tissue: Liver
  map experimental info
  protein estimated location

pI=7.31; Mw=24518

MAPPING (identification):

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
UniProtKB/Swiss-ProtP46527; CDN1B_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCDN1B_HUMAN
Primary accession numberP46527
Secondary accession number(s) Q16307 Q5U0H2 Q9BUS6
Sequence was last modified on November 1, 1995 (version 1)
Annotations were last modified on October 19, 2011 (version 132)
Name and origin of the protein
DescriptionRecName: Full=Cyclin-dependent kinase inhibitor 1B; AltName: Full=Cyclin-dependent kinase inhibitor p27; AltName: Full=p27Kip1;
Gene nameName=CDKN1B
Encoded onName=CDKN1B; Synonyms=KIP1
Keywords3D-structure; Cell cycle; Complete proteome; Cytoplasm; Direct protein sequencing; Endosome; Nucleus; Phosphoprotein; Polymorphism; Protein kinase inhibitor; Proto-oncogene; Reference proteome; Ubl conjugation.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLU10906; AAA20240.1; -; mRNA
EMBLS76988; AAD14244.1; -; Genomic_DNA
EMBLS76986; AAD14244.1; JOINED; Genomic_DNA
EMBLBT019553; AAV38360.1; -; mRNA
EMBLBT019554; AAV38361.1; -; mRNA
EMBLAF480891; AAL78041.1; -; Genomic_DNA
EMBLBC001971; AAH01971.1; -; mRNA
IPIIPI00006991; -; .
RefSeqNP_004055.1; NM_004064.3; .
UniGeneHs.238990; -; .
PDB1H27; X-ray; 2.20 A; E=25-35
PDB1JSU; X-ray; 2.30 A; C=23-106
PDB2AST; X-ray; 2.30 A; D=181-190
PDBsum1H27; -; .
PDBsum1JSU; -; .
PDBsum2AST; -; .
ProteinModelPortalP46527; -; .
SMRP46527; 25-93; .
DisProtDP00018; -; .
DIPDIP-33341N; -; .
IntActP46527; 36; .
MINTMINT-239177; -; .
STRINGP46527; -; .
PhosphoSiteP46527; -; .
SWISS-2DPAGEP46527; -; .
PRIDEP46527; -; .
EnsemblENST00000228872; ENSP00000228872; ENSG00000111276; .
GeneID1027; -; .
KEGGhsa:1027; -; .
UCSCuc001rat.1; human; .
CTD1027; -; .
GeneCardsGC12P012639; -; .
H-InvDBHIX0010441; -; .
HPACAB003691; -; .
HPACAB021888; -; .
MIM600778; gene; .
MIM610755; phenotype; .
neXtProtNX_P46527; -; .
Orphanet652; Multiple endocrine neoplasia type 1; .
PharmGKBPA105; -; .
HOVERGENHBG073988; -; .
OrthoDBEOG4ZGPDC; -; .
PhylomeDBP46527; -; .
Pathway_Interaction_DBpi3kciaktpathway; Class I PI3K signaling events mediated by Akt; .
Pathway_Interaction_DBhnf3apathway; FOXA1 transcription factor network; .
Pathway_Interaction_DBfoxopathway; FoxO family signaling; .
Pathway_Interaction_DBavb3_integrin_pathway; Integrins in angiogenesis; .
Pathway_Interaction_DBtelomerasepathway; Regulation of Telomerase; .
ReactomeREACT_11061; Signalling by NGF; .
ReactomeREACT_152; Cell Cycle; Mitotic; .
ReactomeREACT_1538; Cell Cycle Checkpoints; .
ReactomeREACT_383; DNA Replication; .
ReactomeREACT_9470; Signaling by FGFR; .
NextBio4315; -; .
PMAP-CutDBP46527; -; .
ArrayExpressP46527; -; .
BgeeP46527; -; .
CleanExHS_CDKN1B; -; .
GenevestigatorP46527; -; .
GermOnlineENSG00000111276; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0005768; C:endosome; IEA:UniProtKB-SubCell; .
GOGO:0005654; C:nucleoplasm; TAS:Reactome; .
GOGO:0004693; F:cyclin-dependent protein kinase activity; EXP:Reactome; .
GOGO:0004861; F:cyclin-dependent protein kinase inhibitor activity; TAS:ProtInc; .
GOGO:0019903; F:protein phosphatase binding; IPI:BHF-UCL; .
GOGO:0005072; F:transforming growth factor beta receptor; cytoplasmic mediator activity; TAS:ProtInc
GOGO:0048102; P:autophagic cell death; IDA:BHF-UCL; .
GOGO:0007050; P:cell cycle arrest; IMP:HGNC; .
GOGO:0071285; P:cellular response to lithium ion; IDA:MGI; .
GOGO:0006977; P:DNA damage response; signal transduction by p53 class mediator resulting in cell cycle arrest; TAS:Reactome
GOGO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome; .
GOGO:0000080; P:G1 phase of mitotic cell cycle; TAS:Reactome; .
GOGO:0000082; P:G1/S transition of mitotic cell cycle; IDA:BHF-UCL; .
GOGO:0006917; P:induction of apoptosis; IDA:BHF-UCL; .
GOGO:0030308; P:negative regulation of cell growth; IDA:BHF-UCL; .
GOGO:0045892; P:negative regulation of transcription; DNA-dependent; IDA:UniProtKB
GOGO:0048011; P:nerve growth factor receptor signaling pathway; TAS:Reactome; .
GOGO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome; .
GOGO:0045732; P:positive regulation of protein catabolic process; IDA:MGI; .
GOGO:0000084; P:S phase of mitotic cell cycle; TAS:Reactome; .
InterProIPR003175; CDI; .
PfamPF02234; CDI; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server