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SWISS-2DPAGE

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It will be discontinued on 31-May-2024.


SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P52480




SWISS-2DPAGE:  P52480


P52480


General information about the entry
View entry in simple text format
Entry nameKPYM_MOUSE
Primary accession numberP52480
integrated into SWISS-2DPAGE on June 30, 1999 (release 10)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;.
Gene nameName=Pkm2
Synonyms=Pk3, Pykm
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=7.08; Mw=62739  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=6.80; Mw=56422  [identification data]
pI=6.71; Mw=56578  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
PMMA-2DPAGEP52480.
REPRODUCTION-2DPAGEIPI00225961; IPI00225961.
REPRODUCTION-2DPAGEIPI00845840; IPI00845840.
REPRODUCTION-2DPAGEIPI00407130; IPI00407130.
REPRODUCTION-2DPAGEIPI00116279; IPI00116279.
REPRODUCTION-2DPAGEP52480; P52480.
UniProtKB/Swiss-ProtP52480; KPYM_MOUSE.
World-2DPAGE RepositoryP52480; KPYM_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameKPYM_MOUSE
Primary accession numberP52480
Secondary accession number(s) Q3TBV8 Q3TBW5 Q3TC59 Q3U1X3 Q3U5P6 Q4VC20 Q64484 Q91YI8 Q9CWB1
Sequence was last modified on May 1, 2007 (version 4)
Annotations were last modified on October 19, 2011 (version 125)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;
Gene nameName=Pkm2
Synonyms=Pk3, Pykm
Encoded onName=Pkm2; Synonyms=Pk3, Pykm
KeywordsAcetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD38379; BAA07457.1; -; mRNA
EMBLX97047; CAA65761.1; -; mRNA
EMBLAK002341; BAB22025.1; -; mRNA
EMBLAK135397; BAE22519.1; -; mRNA
EMBLAK151724; BAE30642.1; -; mRNA
EMBLAK153483; BAE32031.1; -; mRNA
EMBLAK155110; BAE33055.1; -; mRNA
EMBLAK155655; BAE33370.1; -; mRNA
EMBLAK170892; BAE42098.1; -; mRNA
EMBLAK168943; BAE40751.1; -; mRNA
EMBLAK171023; BAE42192.1; -; mRNA
EMBLAK171033; BAE42199.1; -; mRNA
EMBLAC160637; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLBC016619; AAH16619.1; -; mRNA
EMBLBC094663; AAH94663.1; -; mRNA
IPIIPI00407130; -; .
IPIIPI00845840; -; .
PIRS55921; S55921; .
RefSeqNP_035229.2; NM_011099.2; .
UniGeneMm.326167; -; .
UniGeneMm.405069; -; .
ProteinModelPortalP52480; -; .
SMRP52480; 13-531; .
IntActP52480; 4; .
MINTMINT-1850796; -; .
STRINGP52480; -; .
PhosphoSiteP52480; -; .
SWISS-2DPAGEP52480; -; .
PMMA-2DPAGEP52480; -; .
REPRODUCTION-2DPAGEIPI00407130; -; .
REPRODUCTION-2DPAGEP52480; -; .
PRIDEP52480; -; .
EnsemblENSMUST00000034834; ENSMUSP00000034834; ENSMUSG00000032294; .
EnsemblENSMUST00000163694; ENSMUSP00000128770; ENSMUSG00000032294; .
GeneID18746; -; .
KEGGmmu:18746; -; .
UCSCuc009pyf.1; mouse; .
UCSCuc009pyi.1; mouse; .
CTD5315; -; .
MGIMGI:97591; Pkm2; .
eggNOGroNOG11660; -; .
GeneTreeENSGT00390000008859; -; .
HOVERGENHBG000941; -; .
InParanoidP52480; -; .
OMAPANEGNT; -; .
OrthoDBEOG40GCQJ; -; .
PhylomeDBP52480; -; .
NextBio294905; -; .
ArrayExpressP52480; -; .
BgeeP52480; -; .
CleanExMM_PKM2; -; .
GenevestigatorP52480; -; .
GermOnlineENSMUSG00000032294; Mus musculus; .
GOGO:0019861; C:flagellum; IDA:MGI; .
GOGO:0005739; C:mitochondrion; IDA:MGI; .
GOGO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0030955; F:potassium ion binding; IEA:InterPro; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0004743; F:pyruvate kinase activity; IDA:MGI; .
GOGO:0006096; P:glycolysis; IDA:MGI; .
InterProIPR001697; Pyr_Knase; .
InterProIPR015813; Pyrv/PenolPyrv_Kinase; .
InterProIPR011037; Pyrv_Knase-like_insert_dom; .
InterProIPR015794; Pyrv_Knase_a/b; .
InterProIPR018209; Pyrv_Knase_AS; .
InterProIPR015793; Pyrv_Knase_brl; .
InterProIPR015795; Pyrv_Knase_C; .
InterProIPR015806; Pyrv_Knase_insert_dom; .
Gene3DG3DSA:2.40.33.10; PK_B_barrel_like; 1; .
Gene3DG3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; .
Gene3DG3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; .
PANTHERPTHR11817; Pyruvate_kinase; 1; .
PfamPF00224; PK; 1; .
PfamPF02887; PK_C; 1; .
PRINTSPR01050; PYRUVTKNASE; .
SUPFAMSSF50800; PK_B_barrel_like; 1; .
SUPFAMSSF52935; Pyruvate_kinase; 1; .
SUPFAMSSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMsTIGR01064; Pyruv_kin; 1; .
PROSITEPS00110; PYRUVATE_KINASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server