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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: P99085



General information about the entry
View entry in simple text format
Entry nameHPRT_STAAN
Primary accession numberP99085
integrated into SWISS-2DPAGE on March 31, 2004 (release 17)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 5)
Name and origin of the protein
DescriptionRecName: Full=Hypoxanthine-guanine phosphoribosyltransferase; Short=HGPRT; Short=HGPRTase; EC=;.
Gene nameName=hpt
Annotated speciesStaphylococcus aureus (strain N315) [TaxID: 158879]
TaxonomyBacteria; Firmicutes; Bacillales; Staphylococcus.
PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, ExPASy, EBI, Israel, Japan]
Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y., Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.
''''''Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth'';'';''
J. Microbiol. Methods 60(2):247-257(2005)
2D PAGE maps for identified proteins
How to interpret a protein

STAPHYLOCOCCUS {Staphylococcus aureus}
Staphylococcus aureus (strain N315)
  map experimental info
  protein estimated location

pI=4.95; Mw=23298  [identification data]

MAPPING (identification):
MALDI tandem mass spectrometry [1].

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
UniProtKB/Swiss-ProtP99085; HPRT_STAAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameHPRT_STAAN
Primary accession numberP99085
Secondary accession number(s) Q99W93
Sequence was last modified on October 11, 2004 (version 1)
Annotations were last modified on October 19, 2011 (version 48)
Name and origin of the protein
DescriptionRecName: Full=Hypoxanthine-guanine phosphoribosyltransferase; Short=HGPRT; Short=HGPRTase; EC=;
Gene nameName=hpt
Encoded onName=hpt; OrderedLocusNames=SA0468
KeywordsComplete proteome; Cytoplasm; Glycosyltransferase; Magnesium; Metal-binding; Nucleotide-binding; Purine salvage; Transferase.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLBA000018; BAB41698.1; -; Genomic_DNA
PIRG89817; G89817; .
RefSeqNP_373720.1; NC_002745.2; .
ProteinModelPortalP99085; -; .
STRINGP99085; -; .
SWISS-2DPAGEP99085; -; .
EnsemblBacteriaEBSTAT00000002295; EBSTAP00000002295; EBSTAG00000002295; .
GeneID1123259; -; .
GenomeReviewsBA000018_GR; SA0468; .
KEGGsau:SA0468; -; .
eggNOGCOG0634; -; .
GeneTreeEBGT00050000024017; -; .
HOGENOMHBG536385; -; .
ProtClustDBCLSK884701; -; .
BioCycSAUR158879:SA0468-MONOMER; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0052657; F:guanine phosphoribosyltransferase activity; IEA:EC; .
GOGO:0004422; F:hypoxanthine phosphoribosyltransferase activity; IEA:EC; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0000166; F:nucleotide binding; IEA:UniProtKB-KW; .
GOGO:0006166; P:purine ribonucleoside salvage; IEA:UniProtKB-KW; .
InterProIPR005904; Hxn_phspho_trans; .
InterProIPR000836; PRibTrfase; .
PfamPF00156; Pribosyltran; 1; .
TIGRFAMsTIGR01203; HGPRTase; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server