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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: APT_ECOLI



General information about the entry
View entry in simple text format
Entry nameAPT_ECOLI
Primary accession numberP69503
Secondary accession number(s) P07672
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=Adenine phosphoribosyltransferase; Short=APRT; EC=;.
Gene nameName=apt
OrderedLocusNames=b0469, JW0458
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
  map experimental info
  protein estimated location

pI=5.30; Mw=23792  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [1].

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
UniProtKB/Swiss-ProtP69503; APT_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameAPT_ECOLI
Primary accession numberP69503
Secondary accession number(s) P07672 P09993 P77121 Q2MBV8
Sequence was last modified on April 1, 1988 (version 1)
Annotations were last modified on October 19, 2011 (version 62)
Name and origin of the protein
DescriptionRecName: Full=Adenine phosphoribosyltransferase; Short=APRT; EC=;
Gene nameName=apt
OrderedLocusNames=b0469, JW0458
Encoded onName=apt; OrderedLocusNames=b0469, JW0458
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Glycosyltransferase; Purine salvage; Reference proteome; Transferase.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLM14040; AAA23455.1; -; Genomic_DNA
EMBLX04487; CAA28173.1; -; Genomic_DNA
EMBLU82664; AAB40223.1; ALT_INIT; Genomic_DNA
EMBLU00096; AAC73571.1; -; Genomic_DNA
EMBLAP009048; BAE76248.1; -; Genomic_DNA
EMBLM38777; AAA23456.1; -; Genomic_DNA
PIRA25635; RTECA; .
RefSeqNP_415002.1; NC_000913.2; .
PDB2DY0; X-ray; 1.25 A; A/B=1-183
PDBsum2DY0; -; .
ProteinModelPortalP69503; -; .
SMRP69503; 2-183; .
DIPDIP-36165N; -; .
IntActP69503; 5; .
SWISS-2DPAGEP69503; -; .
PRIDEP69503; -; .
EnsemblBacteriaEBESCT00000001461; EBESCP00000001461; EBESCG00000001216; .
EnsemblBacteriaEBESCT00000015958; EBESCP00000015249; EBESCG00000015018; .
GeneID945113; -; .
GenomeReviewsAP009048_GR; JW0458; .
GenomeReviewsU00096_GR; b0469; .
KEGGecj:JW0458; -; .
KEGGeco:b0469; -; .
EchoBASEEB0049; -; .
EcoGeneEG10051; apt; .
eggNOGCOG0503; -; .
GeneTreeEBGT00050000010453; -; .
HOGENOMHBG703830; -; .
ProtClustDBPRK02304; -; .
GenevestigatorP69503; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0003999; F:adenine phosphoribosyltransferase activity; IDA:EcoCyc; .
GOGO:0042802; F:identical protein binding; IDA:EcoCyc; .
GOGO:0000287; F:magnesium ion binding; IDA:EcoCyc; .
GOGO:0006168; P:adenine salvage; IDA:EcoCyc; .
GOGO:0006166; P:purine ribonucleoside salvage; IEA:UniProtKB-KW; .
HAMAPMF_00004; Aden_phosphoribosyltr; 1; -
InterProIPR005764; Ade_phspho_trans; .
InterProIPR000836; PRibTrfase; .
PfamPF00156; Pribosyltran; 1; .
TIGRFAMsTIGR01090; Apt; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server