Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P09103:

ID   PDIA1_MOUSE; STANDARD; 2DG.
AC   P09103;
DT   01-SEP-1998, integrated into SWISS-2DPAGE (release 7).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 10.
DE   RecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1;
DE   AltName: Full=Cellular thyroid hormone-binding protein; AltName:
DE   Full=Endoplasmic reticulum resident protein 59; Short=ER protein 59;
DE   Short=ERp59; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName:
DE   Full=p55; Flags: Precursor;.
GN   Name=P4hb; Synonyms=Pdia1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
MT   ISLETS_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, WAT_MOUSE.
IM   ISLETS_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, WAT_MOUSE.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96154592; PubMed=8586073; [NCBI, Expasy, EBI, Israel, Japan]
RA   Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers
RA   J., Steiner S., Witzmann F., Anderson N.G.;
RT   ''''''An updated two-dimensional gel database of rat liver proteins
RT   useful in gene regulation and drug effect studies'';'';'';
RL   Electrophoresis 16(1):1977-1981(1995).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano
RA   S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A.,
RA   Hochstrasser D.F.;
RT   ''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of
RT   diabetes and obesity'';'';'';
RL   Proteomics 1(1):136-163(2001).
2D   -!- MASTER: ISLETS_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0018EK=4.96/62422;
2D   -!-   PI/MW: SPOT 2D-0018IO=4.91/57522;
2D   -!-   PI/MW: SPOT 2D-0018IP=4.92/57048;
2D   -!-   PI/MW: SPOT 2D-0018IS=4.94/57048;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0018EK: 910.518; 966.626; 1002.64;
2D         1066.577; 1666.88; 1780.863; 1833.93; 1965.043; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-0018IS: 928.476; 962.419; 966.533; 970.495;
2D         1002.524; 1051.489; 1066.513; 1081.628; 1202.592; 1206.598; 1216.595;
2D         1222.602; 1309.624; 1344.678; 1409.67; 1424.758; 1486.756; 1666.833;
2D         1743.921; 1780.852; 1794.886; 1833.93; 1965.051; TRYPSIN.
2D   -!-   MAPPING: SPOTS 2D-0018EK, 2D-0018IS: Peptide mass fingerprinting
2D         [2]; SPOTS 2D-001*: MATCHING WITH THE MOUSE LIVER MASTER GEL [2].
2D   -!- MASTER: LIVER_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0013BT=4.86/55474;
2D   -!-   PI/MW: SPOT 2D-0013BV=4.82/55784;
2D   -!-   PI/MW: SPOT 2D-0013BW=4.88/55474;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0013BT: 905.318; 910.361; 966.512;
2D         1051.491; 1066.5; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-0013BV: 850.47; 905.518; 910.461; 966.582;
2D         1066.542; 1216.615; 1666.87; 1833.911; 1965.099; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-0013BW: 910.428; 928.495; 966.543; 970.503;
2D         1066.506; 1202.568; 1216.586; 1666.843; 1780.83; 1833.923; 1965.082; TRYPSIN.
2D   -!-   MAPPING: MATCHING WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1],
2D         MICROSEQUENCING AND MASS FINGERPRINTING [2].
2D   -!- MASTER: MUSCLE_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0016R9=4.77/57681;
2D   -!-   PI/MW: SPOT 2D-0016RM=4.83/57205;
2D   -!-   MAPPING: MATCHING WITH THE MOUSE LIVER MASTER GEL [2].
2D   -!- MASTER: WAT_MOUSE;
2D   -!-   PI/MW: SPOT 2D-001AYV=4.91/55685;
2D   -!-   PI/MW: SPOT 2D-001AZ8=4.93/55510;
2D   -!-   MAPPING: MATCHING WITH THE MOUSE LIVER MASTER GEL [2].
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DR   COMPLUYEAST-2DPAGE; P09103; PDIA1_MOUSE.
DR   REPRODUCTION-2DPAGE; P09103; P09103.
DR   UniProtKB/Swiss-Prot; P09103; PDIA1_MOUSE.
//

The following 2-D maps are available for this protein:

	Pancreatic islet cells	Liver	Gastrocnemius muscle
	White adipose tissue

SWISS-2DPAGE (search AC)