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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P0ABB4 |
SWISS-2DPAGE: P0ABB4
P0ABB4:
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ID ATPB_ECOLI; STANDARD; 2DG.
AC P0ABB4; P00824;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 6.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=ATP synthase subunit beta; EC=3.6.3.14; AltName: Full=ATP
DE synthase F1 sector subunit beta; AltName: Full=F-ATPase subunit beta;.
GN Name=atpD; Synonyms=papB, uncD; OrderedLocusNames=b3732, JW3710;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [4]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: F-TYPE ATPASES HAVE 2 COMPONENTS, CF(1) - THE CATALYTIC CORE
CC - AND CF(0) - THE MEMBRANE PROTON CHANNEL; CF(1) HAS FIVE SUBUNITS:
CC ALPHA(3), BETA(3), GAMMA(1), DELTA(1), EPSILON(1); CF(0) HAS THREE MAIN
CC SUBUNITS: A, B AND C
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KUF=4.90/47816;
2D -!- MAPPING: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND
2D IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HHU=4.95/47782;
2D -!- PI/MW: SPOT 2D-001HJ0=4.95/43643;
2D -!- PI/MW: SPOT 2D-001HJ1=4.55/43745;
2D -!- PEPTIDE MASSES: SPOT 2D-001HHU: 1366.714; 1450.812; 1506.716;
2D 1608.946; 1676.778; 1790.852; 1905.953; 2151.245; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HJ0: 900.518; 1366.767; 1450.804;
2D 1506.752; 1608.969; 1676.814; 1840.897; 1905.971; 2003.052; 2151.196;
2D 2179.13; 2338.165; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HJ1: 900.551; 918.458; 1107.594;
2D 1122.619; 1263.73; 1332.747; 1366.782; 1450.851; 1506.781; 1609.017;
2D 1660.888; 1676.887; 1824.043; 1840.995; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [3]; SPOT 2D-001HJ0: GEL
2D MATCHING WITH MASTER ECOLI4.5-5.5 [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014XV=5.00/45494;
2D -!- PI/MW: SPOT 2D-0014ZV=4.96/42905;
2D -!- PEPTIDE MASSES: SPOT 2D-0014XV: 1366.61; 1450.65; 1506.57; 1608.78;
2D 1676.65; 1840.72; 1905.72; 2002.83; 2150.93; 2178.87; 2337.89; 2345.01; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting and microsequencing (ATGKI) [3].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WJK=4.99/48103;
2D -!- PI/MW: SPOT 2D-001WJO=4.96/48103;
2D -!- PEPTIDE MASSES: SPOT 2D-001WJK: 900.47; 961.45; 1094.53; 1366.61;
2D 1450.66; 1506.59; 1608.8; 1823.74; 1840.74; 1905.75; 1957.81; 2002.82;
2D 2150.96; 2178.84; 3816.37; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WJO: 900.55; 961.51; 1138.57; 1831.03;
2D 1841.01; 2003.13; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WJK: (K)IVQVIGAVVDVEFPQDAVPR(V),5-24;
2D (R)VYDALEVQNGNER(L),25-37; (R)LVLEVQQQLGGGIVR(T),38-52;
2D (R)GLDVKDLEHPIEVPVGK(A),65-81; (K)VGLFGGAGVGK(T),145-155;
2D (R)NIAIEHSGYSVFAGVGER(T),165-182; (R)YTLAGTEVSALLGR(M),247-260;
2D (R)QIASLGIYPAVDPLDSTSR(Q),324-342; (R)GVQSILQR(Y),359-366; (R)FLSQPFFVAEVFTGSPGK(Y),399-416.
2D -!- EXPRESSION: decrease after benzoic acid treatment [4].
2D -!- MAPPING: Peptide mass fingerprinting [4]; SPOT 2D-001WJK: TANDEM
2D MASS SPECTROMETRY [4].
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DR 2DBase-Ecoli; P0ABB4; ATPB_ECOLI.
DR ECO2DBASE; B046.7; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0ABB4; ATPB_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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