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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0ACF8




SWISS-2DPAGE:  P0ACF8


P0ACF8


General information about the entry
View entry in simple text format
Entry nameHNS_ECOLI
Primary accession numberP0ACF8
Secondary accession number(s) P08936
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=DNA-binding protein H-NS; AltName: Full=Histone-like protein HLP-II; AltName: Full=Protein B1; AltName: Full=Protein H1;.
Gene nameName=hns
Synonyms=bglY, cur, drdX, hnsA, msyA, osmZ, pilG, topS
OrderedLocusNames=b1237, JW1225
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.38; Mw=15660  [identification data]
pI=5.45; Mw=15616
pI=5.00; Mw=12496
pI=5.02; Mw=10782  [identification data]

MAPPING (identification):
SPOT 2D-000LQK: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]; SPOTS 2D-000LQS, 2D-000LTS: GEL MATCHING, AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2];
SPOT 2D-000LV7: AMINO ACID COMPOSITION AND SEQUENCE TAG (SEAL) [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
ECO2DBASEE014.0; 6TH EDITION.
ECO2DBASEF014.7; 6TH EDITION.
UniProtKB/Swiss-ProtP0ACF8; HNS_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameHNS_ECOLI
Primary accession numberP0ACF8
Secondary accession number(s) P08936
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 59)
Name and origin of the protein
DescriptionRecName: Full=DNA-binding protein H-NS; AltName: Full=Histone-like protein HLP-II; AltName: Full=Protein B1; AltName: Full=Protein H1;
Gene nameName=hns
Synonyms=bglY, cur, drdX, hnsA, msyA, osmZ, pilG, topS
OrderedLocusNames=b1237, JW1225
Encoded onName=hns; Synonyms=bglY, cur, drdX, hnsA, msyA, osmZ, pilG, topS; OrderedLocusNames=b1237, JW1225
Keywords3D-structure; Complete proteome; Direct protein sequencing; DNA-binding; Reference proteome; Repressor; Transcription; Transcription regulation.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX07688; CAA30530.1; -; Genomic_DNA
EMBLX59940; CAA42565.1; -; Genomic_DNA
EMBLX57231; CAA40507.1; -; Genomic_DNA
EMBLX67326; CAA47740.1; -; Genomic_DNA
EMBLU00096; AAC74319.1; -; Genomic_DNA
EMBLAP009048; BAA36117.1; -; Genomic_DNA
PIRS00903; S00903; .
RefSeqNP_415753.1; NC_000913.2; .
PDB1HNR; NMR; -; A=91-137
PDB1HNS; NMR; -; A=91-137
PDB1LR1; NMR; -; A/B=2-57
PDB1NI8; NMR; -; A/B=2-46
PDBsum1HNR; -; .
PDBsum1HNS; -; .
PDBsum1LR1; -; .
PDBsum1NI8; -; .
ProteinModelPortalP0ACF8; -; .
SMRP0ACF8; 2-81; 91-137; .
DIPDIP-35853N; -; .
IntActP0ACF8; 47; .
MINTMINT-1223907; -; .
SWISS-2DPAGEP0ACF8; -; .
ECO2DBASEE014.0; 6TH EDITION; .
ECO2DBASEF014.7; 6TH EDITION; .
EnsemblBacteriaEBESCT00000001738; EBESCP00000001738; EBESCG00000001431; .
EnsemblBacteriaEBESCT00000016273; EBESCP00000015564; EBESCG00000015333; .
GeneID945829; -; .
GenomeReviewsAP009048_GR; JW1225; .
GenomeReviewsU00096_GR; b1237; .
KEGGecj:JW1225; -; .
KEGGeco:b1237; -; .
EchoBASEEB0452; -; .
EcoGeneEG10457; hns; .
eggNOGCOG2916; -; .
GeneTreeEBGT00050000008684; -; .
HOGENOMHBG740436; -; .
OMAQYRDMLI; -; .
ProtClustDBPRK10947; -; .
BioCycEcoCyc:PD00288; -; .
GenevestigatorP0ACF8; -; .
GOGO:0005622; C:intracellular; IEA:InterPro; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0003677; F:DNA binding; IEA:UniProtKB-KW; .
GOGO:0006355; P:regulation of transcription; DNA-dependent; IEA:UniProtKB-KW
GOGO:0006351; P:transcription; DNA-dependent; IEA:UniProtKB-KW
InterProIPR001801; Histone_HNS; .
PfamPF00816; Histone_HNS; 1; .
SMARTSM00528; HNS; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server