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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P0AFG8 |
SWISS-2DPAGE: P0AFG8
P0AFG8:
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ID ODP1_ECOLI; STANDARD; 2DG.
AC P0AFG8; P06958;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=Pyruvate dehydrogenase E1 component; EC=1.2.4.1;.
GN Name=aceE; OrderedLocusNames=b0114, JW0110;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP PROTEIN EXPRESSION.
RX MEDLINE=83160794; PubMed=6339477; [NCBI, Expasy, EBI, Israel, Japan]
RA Smith M.W., Neidhardt F.C.;
RT ''''''Proteins induced by aerobiosis in Escherichia coli'';'';'';
RL J. Bacteriol. 154(1):344-350(1983).
RN [4]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMODIMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KBO=5.40/99256;
2D -!- EXPRESSION: INCREASED DURING AEROBIC GROWTH [3].
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WBM=5.39/94195;
2D -!- PEPTIDE MASSES: SPOT 2D-001WBM: 921.47; 964.49; 1073.47; 1096.59;
2D 1099.49; 1136.58; 1142.69; 1263.61; 1288.61; 1302.61; 1333.71; 1503.74;
2D 1964.96; TRYPSIN.
2D -!- EXPRESSION: increase after benzoic acid treatment [4].
2D -!- MAPPING: Peptide mass fingerprinting [4].
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DR 2DBase-Ecoli; P0AFG8; ODP1_ECOLI.
DR ECO2DBASE; F099.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0AFG8; ODP1_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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