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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P14174




SWISS-2DPAGE:  P14174


P14174


General information about the entry
View entry in simple text format
Entry nameMIF_HUMAN
Primary accession numberP14174
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Macrophage migration inhibitory factor; Short=MIF; EC=5.3.2.1; AltName: Full=Glycosylation-inhibiting factor; Short=GIF; AltName: Full=L-dopachrome isomerase; AltName: Full=L-dopachrome tautomerase; EC=5.3.3.12; AltName: Full=Phenylpyruvate tautomerase;.
Gene nameName=MIF
Synonyms=GLIF, MMIF
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
Comments
  • SUBUNIT: POSSIBLE DIMER
2D PAGE maps for identified proteins
How to interpret a protein

LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=6.35; Mw=11731

PATHOLOGICAL LEVEL:
INCREASED DURING CHRONIC INFLAMMATORY LOCI [1].

MAPPING (identification):
MICROSEQUENCING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
Siena-2DPAGEP14174; MIF_HUMAN.
UniProtKB/Swiss-ProtP14174; MIF_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameMIF_HUMAN
Primary accession numberP14174
Secondary accession number(s) A5Z1R8 B2R4S3 Q2V4Y5 Q6FHV0
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on October 19, 2011 (version 143)
Name and origin of the protein
DescriptionRecName: Full=Macrophage migration inhibitory factor; Short=MIF; EC=5.3.2.1; AltName: Full=Glycosylation-inhibiting factor; Short=GIF; AltName: Full=L-dopachrome isomerase; AltName: Full=L-dopachrome tautomerase; EC=5.3.3.12; AltName: Full=Phenylpyruvate tautomerase;
Gene nameName=MIF
Synonyms=GLIF, MMIF
Encoded onName=MIF; Synonyms=GLIF, MMIF
Keywords3D-structure; Acetylation; Complete proteome; Cytokine; Cytoplasm; Direct protein sequencing; Immunity; Inflammatory response; Innate immunity; Isomerase; Phosphoprotein; Reference proteome; Secreted.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM25639; AAA36315.1; -; mRNA
EMBLL10612; AAA35892.1; -; mRNA
EMBLZ23063; CAA80598.1; -; mRNA
EMBLL19686; AAA21814.1; -; Genomic_DNA
EMBLAF469046; AAL78635.1; -; mRNA
EMBLEF611126; ABQ95571.1; -; mRNA
EMBLCR456520; CAG30406.1; -; mRNA
EMBLAK311929; BAG34870.1; -; mRNA
EMBLCR407644; CAG28572.1; -; mRNA
EMBLCR541651; CAG46452.1; -; mRNA
EMBLBT007148; AAP35812.1; -; mRNA
EMBLDQ307455; ABB96245.1; -; Genomic_DNA
EMBLCH471095; EAW59620.1; -; Genomic_DNA
EMBLBC000447; AAH00447.1; -; mRNA
EMBLBC007676; AAH07676.1; -; mRNA
EMBLBC008914; AAH08914.1; -; mRNA
EMBLBC013976; AAH13976.1; -; mRNA
EMBLBC022414; AAH22414.1; -; mRNA
EMBLBC053376; AAH53376.1; -; mRNA
EMBLM95775; AAA36179.1; -; mRNA
IPIIPI00293276; -; .
PIRA48793; A48793; .
RefSeqNP_002406.1; NM_002415.1; .
UniGeneHs.407995; -; .
PDB1CA7; X-ray; 2.50 A; A/B/C=2-115
PDB1CGQ; X-ray; 2.00 A; A/B/C=3-115
PDB1GCZ; X-ray; 1.90 A; A/B/C=2-115
PDB1GD0; X-ray; 1.50 A; A/B/C=2-115
PDB1GIF; X-ray; 1.90 A; A/B/C=1-115
PDB1LJT; X-ray; 2.00 A; A/B/C=2-114
PDB1MIF; X-ray; 2.60 A; A/B/C=1-115
PDB1P1G; X-ray; 2.50 A; A/B/C=3-115
PDB2OOH; X-ray; 1.85 A; A/B/C=2-115
PDB2OOW; X-ray; 1.75 A; A/B/C=2-115
PDB2OOZ; X-ray; 1.80 A; A/B/C=2-115
PDB3B9S; X-ray; 1.80 A; A/B/C=2-115
PDB3CE4; X-ray; 1.55 A; A/B/C=2-115
PDB3DJH; X-ray; 1.25 A; A/B/C=2-115
PDB3DJI; X-ray; 1.95 A; A/B/C/D/E/F=2-115
PDB3HOF; X-ray; 1.90 A; A/B/C=1-115
PDB3IJG; X-ray; 1.70 A; A/B/C=2-115
PDB3IJJ; X-ray; 1.25 A; A/B/C=2-115
PDB3JSF; X-ray; 1.93 A; A/B/C=2-115
PDB3JSG; X-ray; 1.58 A; A/B/C=2-115
PDB3JTU; X-ray; 1.86 A; A/B/C=2-115
PDB3L5P; X-ray; 1.80 A; A/B/C=2-115
PDB3L5R; X-ray; 1.94 A; A/B/C=2-115
PDB3L5S; X-ray; 1.86 A; A/B/C=2-115
PDB3L5T; X-ray; 1.86 A; A/B/C=2-115
PDB3L5U; X-ray; 1.90 A; A/B/C=2-115
PDB3L5V; X-ray; 1.70 A; A/B/C=2-115
PDBsum1CA7; -; .
PDBsum1CGQ; -; .
PDBsum1GCZ; -; .
PDBsum1GD0; -; .
PDBsum1GIF; -; .
PDBsum1LJT; -; .
PDBsum1MIF; -; .
PDBsum1P1G; -; .
PDBsum2OOH; -; .
PDBsum2OOW; -; .
PDBsum2OOZ; -; .
PDBsum3B9S; -; .
PDBsum3CE4; -; .
PDBsum3DJH; -; .
PDBsum3DJI; -; .
PDBsum3HOF; -; .
PDBsum3IJG; -; .
PDBsum3IJJ; -; .
PDBsum3JSF; -; .
PDBsum3JSG; -; .
PDBsum3JTU; -; .
PDBsum3L5P; -; .
PDBsum3L5R; -; .
PDBsum3L5S; -; .
PDBsum3L5T; -; .
PDBsum3L5U; -; .
PDBsum3L5V; -; .
ProteinModelPortalP14174; -; .
SMRP14174; 2-115; .
IntActP14174; 15; .
MINTMINT-5000040; -; .
STRINGP14174; -; .
PhosphoSiteP14174; -; .
SWISS-2DPAGEP14174; -; .
Siena-2DPAGEP14174; -; .
PeptideAtlasP14174; -; .
PRIDEP14174; -; .
EnsemblENST00000215754; ENSP00000215754; ENSG00000240972; .
GeneID4282; -; .
KEGGhsa:4282; -; .
UCSCuc002zyr.1; human; .
CTD4282; -; .
GeneCardsGC22P007221; -; .
H-InvDBHIX0041297; -; .
HGNCHGNC:7097; MIF; .
HPACAB005284; -; .
HPAHPA003868; -; .
MIM153620; gene; .
MIM604302; phenotype; .
neXtProtNX_P14174; -; .
Orphanet92; Juvenile idiopathic arthritis; .
PharmGKBPA30819; -; .
eggNOGprNOG20415; -; .
GeneTreeENSGT00530000063544; -; .
HOVERGENHBG003240; -; .
InParanoidP14174; -; .
OrthoDBEOG49GKJ5; -; .
PhylomeDBP14174; -; .
NextBio16845; -; .
ArrayExpressP14174; -; .
CleanExHS_MIF; -; .
GenevestigatorP14174; -; .
GOGO:0009986; C:cell surface; IDA:BHF-UCL; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0005615; C:extracellular space; IEA:UniProtKB-KW; .
GOGO:0043498; F:cell surface binding; IDA:BHF-UCL; .
GOGO:0042056; F:chemoattractant activity; IDA:BHF-UCL; .
GOGO:0005125; F:cytokine activity; IDA:UniProtKB; .
GOGO:0005126; F:cytokine receptor binding; IPI:BHF-UCL; .
GOGO:0004167; F:dopachrome isomerase activity; IDA:UniProtKB; .
GOGO:0050178; F:phenylpyruvate tautomerase activity; IDA:MGI; .
GOGO:0008283; P:cell proliferation; IDA:UniProtKB; .
GOGO:0007166; P:cell surface receptor linked signaling pathway; IDA:UniProtKB; .
GOGO:0006954; P:inflammatory response; IEA:UniProtKB-KW; .
GOGO:0045087; P:innate immune response; IEA:UniProtKB-KW; .
GOGO:0043066; P:negative regulation of apoptosis; IDA:UniProtKB; .
GOGO:0090344; P:negative regulation of cell aging; IDA:BHF-UCL; .
GOGO:0071157; P:negative regulation of cell cycle arrest; IDA:BHF-UCL; .
GOGO:0043518; P:negative regulation of DNA damage response; signal transduction by p53 class mediator; IDA:BHF-UCL
GOGO:0010629; P:negative regulation of gene expression; IDA:BHF-UCL; .
GOGO:0030890; P:positive regulation of B cell proliferation; IDA:BHF-UCL; .
GOGO:0050715; P:positive regulation of cytokine secretion; IDA:BHF-UCL; .
GOGO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:BHF-UCL; .
GOGO:0048146; P:positive regulation of fibroblast proliferation; IDA:BHF-UCL; .
GOGO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IDA:BHF-UCL; .
GOGO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:BHF-UCL; .
GOGO:0010739; P:positive regulation of protein kinase A signaling cascade; IDA:BHF-UCL; .
GOGO:0001516; P:prostaglandin biosynthetic process; IDA:UniProtKB; .
GOGO:0070207; P:protein homotrimerization; IPI:UniProtKB; .
GOGO:0043030; P:regulation of macrophage activation; NAS:UniProtKB; .
InterProIPR001398; Macrophage_inhib_fac; .
InterProIPR019829; Macrophage_inhib_fac_CS; .
InterProIPR014347; Tautomerase; .
Gene3DG3DSA:3.30.429.10; Tautomerase; 1; .
PANTHERPTHR11954; MIF; 1; .
PfamPF01187; MIF; 1; .
ProDomPD004816; Macrophage_inhib_fac; 1; .
SUPFAMSSF55331; SSF55331; 1; .
PROSITEPS01158; MIF; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server