Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P75691:

ID   YAHK_ECOLI; STANDARD; 2DG.
AC   P75691;
DT   15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT   31-MAR-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 7.
DE   RecName: Full=Uncharacterized zinc-type alcohol dehydrogenase-like
DE   protein YahK; EC=1.-.-.-;.
GN   Name=yahK; OrderedLocusNames=b0325, JW0317;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI-DIGE4.5-6.5.
IM   ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WM3=5.82/42128;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WM3: 855.38; 880.38; 1011.46; 1043.47;
2D         1082.47; 1130.48; 1208.45; 2056.79; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WM3: (K)YAPGDLVGVGCIVDSCK(H),78-94; (K)ALGADEVVNSR(N),216-226.
2D   -!-   MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; P75691; YAHK_ECOLI.
//

The following 2-D map is available for this protein:

	Escherichia coli DIGE (4.5-6.5)

SWISS-2DPAGE (search AC)