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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P77258 |
SWISS-2DPAGE: P77258
P77258:
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ID NEMA_ECOLI; STANDARD; 2DG.
AC P77258;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 7.
DE RecName: Full=N-ethylmaleimide reductase; EC=1.-.-.-; AltName:
DE Full=N-ethylmaleimide reducing enzyme;.
GN Name=nemA; Synonyms=ydhN; OrderedLocusNames=b1650, JW1642;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WN7=5.78/39021;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WN7: (K)VGAITAANR(I),12-20;
2D (R)ASAGLIISEATQISAQAK(G),50-67; (R)ISHASLQPGGQAPVAPSALSAGTR(T),108-131;
2D (R)ALELEEIPGIVNDFR(Q),151-165; (K)GLIDAVAFGR(D),316-325;
2D (R)DWIANPDLVAR(L),326-336; (R)KAELNPQR(A),340-347.
2D -!- MAPPING: Tandem mass spectrometry [1].
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DR UniProtKB/Swiss-Prot; P77258; NEMA_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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