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Sample Preparation and Post-separation Analysis

P99063:

ID   ODPB_STAAN; STANDARD; 2DG.
AC   P99063;
DT   31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT   31-MAR-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 4.
DE   RecName: Full=Pyruvate dehydrogenase E1 component subunit beta; EC=1.2.4.1;.
GN   Name=pdhB; OrderedLocusNames=SA0944;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
MT   STAPHYLOCOCCUS.
IM   STAPHYLOCOCCUS.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA   Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA   J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT   ''''''Correlation of proteomic and transcriptomic profiles of
RT   Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL   J. Microbiol. Methods 60(2):247-257(2005).
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain 
2D   -!- MASTER: STAPHYLOCOCCUS;
2D   -!-   PI/MW: SPOT 2D-001XZP=5.01/33544;
2D   -!-   PI/MW: SPOT 2D-001XZS=4.95/33439;
2D   -!-   PI/MW: SPOT 2D-001XZT=4.90/33596;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XZP: (M)AQMTMVQAINDALK(T),2-15;
2D         (K)VVIPSGPYDAK(G),145-155; (R)SNDPVVYLEHMK(L),164-175;
2D         (R)SNDPVVYLEHMoxK(L),164-175; (R)EEVPEEEYTIDIGK(A),182-195;
2D         (K)AAEELEKDGYSVEVIDLR(T),220-237; (R)TVQPIDVDTIVASVEK(T),238-253;
2D         (R)AVVVQEAQR(Q),257-265; (R)QAGVGAAVVAELSER(A),266-280; (R)AILSLEAPIGR(V),281-291.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XZS: (R)SFREEVPEEEYTIDIGK(A),179-195;
2D         (R)EEVPEEEYTIDIGK(A),182-195; (R)TVQPIDVDTIVASVEK(T),238-253;
2D         (R)QAGVGAAVVAELSER(A),266-280; (R)AILSLEAPIGR(V),281-291.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XZT: (K)VVIPSGPYDAK(G),145-155;
2D         (R)SFREEVPEEEYTIDIGK(A),179-195; (R)AVVVQEAQR(Q),257-265;
2D         (R)QAGVGAAVVAELSER(A),266-280; (R)AILSLEAPIGR(V),281-291.
2D   -!-   MAPPING: MALDI tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; P99063; ODPB_STAAN.
//

The following 2-D map is available for this protein:

	Staphylococcus aureus

SWISS-2DPAGE (search AC)