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Sample Preparation and Post-separation Analysis

P99078:

ID   G6PI_STAAN; STANDARD; 2DG.
AC   P99078;
DT   31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT   31-MAR-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 5.
DE   RecName: Full=Glucose-6-phosphate isomerase; Short=GPI; EC=5.3.1.9;
DE   AltName: Full=Phosphoglucose isomerase; Short=PGI; AltName:
DE   Full=Phosphohexose isomerase; Short=PHI;.
GN   Name=pgi; OrderedLocusNames=SA0823;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
MT   STAPHYLOCOCCUS.
IM   STAPHYLOCOCCUS.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA   Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA   J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT   ''''''Correlation of proteomic and transcriptomic profiles of
RT   Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL   J. Microbiol. Methods 60(2):247-257(2005).
2D   -!- MASTER: STAPHYLOCOCCUS;
2D   -!-   PI/MW: SPOT 2D-001XWE=4.83/41915;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XWE: (K)TLEFFGEHELK(Q),11-21;
2D         (K)ELVDYLADKDFSVNVISK(S),122-139; (K)SGTTTEPAVAFR(L),140-151;
2D         (K)QLATNEGYETFIVPDDVGGR(Y),183-202; (K)QLFGESEGKDFK(G),281-292; (R)FLFETVVK(V),318-325.
2D   -!-   MAPPING: MALDI tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; P99078; G6PI_STAAN.
//

The following 2-D map is available for this protein:

	Staphylococcus aureus

SWISS-2DPAGE (search AC)